β-Cyclodextrin Nanosponges Inclusion Compounds Associated with Silver Nanoparticles to Increase the Antimicrobial Activity of Quercetin
Abstract
:1. Introduction
- The development of an NSs–QRC formulation to improve the stability of free QRC.
- The association of AgNPs to the NSs–QRC complex to generate the AgNPs–NSs–QRC ternary system and the further evaluation of its antimicrobial properties.
- Evaluating the biocompatibility and biotoxicity of the NS–QRC and AgNPs–NSs–QRC complexes.
2. Materials and Methods
2.1. Materials
2.2. Synthesis of AgNPs
2.3. Synthesis and Purification of the NSs Matrix
2.4. Formation of the NSs-QRC Inclusion Compound
2.5. Formation of the ICs–AgNPs Ternary Complexes
2.6. Characterization Methods
2.7. Complexation Efficiencies of the NS–QRC Formulation
2.8. Antibacterial Activity
2.9. MTS Cell Metabolic Activity Assay
2.10. Statistical Analysis
3. Results and Discussion
3.1. Characterization of the ICs
3.1.1. FE–SEM of the NS–QRC Complex
3.1.2. TGA of the NS–QRC Complexes
3.1.3. XRPD of the NS–QRC Complexes
3.1.4. Loading Capacity (LC%) and Encapsulation Efficiencies (EE%) of the NS–QRC Complex
3.1.5. 1H–NMR of the NS–QRC Complexes
3.2. Characterization of NS–QRC Associated with AgNPs
3.2.1. TEM, UV–Vis, XRPD, SAED, DLS, and ζ-Potential of AgNPs
3.2.2. TEM of the NS–QRC Complex Associated to the AgNPs
3.2.3. FE–SEM and EDS Analyses of NS–QRC Associated with AgNPs
3.2.4. UV–Visible Spectrum of NS–QRC Associated with AgNPs
3.2.5. DLS and ζ-Potential of NS–QRC Associated to the AgNPs
3.3. Antibacterial Assays of NS–QRC Associated with the AgNPs
3.4. Cell Metabolic Activity Assays
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
FE–SEM Images of Free NSs
Appendix B
Determination of QRC Content Using Derivative Thermogravimetry (DTG)
Appendix C
UV–Visible Spectra, DLS, and ζ-Potentials of AgNP–NS–QRC after 20 Days of Storage
Sample | Dh (nm) | ζ-Potentials (mV) | PDI |
---|---|---|---|
AgNP–NS–QRC (Day 0) | 261 ± 11 | −28 ± 5 | 0.48 |
AgNP–NS–QRC (Day 20) | 273 ± 15 | −23 ± 8 | 0.56 |
Appendix D
Cumulative Release of QRC from the NS–QRC and AgNP–QRC–NS Complexes
Appendix E
Selected Images for the Antimicrobial Activity Experiments
Appendix F
Selected Images from MTS Assay
References
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Sample | Decomposition Temperature (°C) |
---|---|
QRC | 157.9–177.3 |
QRC | 305.3–313.8 |
NSs | 352.7–353.1 |
NSs-QRC | 355.9–369.6 |
Sample | RDC (%) |
---|---|
QRC | 83.3 ± 4.7 |
NSs | 30.1 ± 2.3 |
NS–QRC | 27.7 ± 5.9 |
Sample | Encapsulation Efficiency (%) | Loading Capacity (%) |
---|---|---|
β–CD–QRC | 60.5 ± 2.2 | 5.77 ± 1.8 |
NS–QRC | 88.1 ± 3.9 | 17.7 ± 3.5 |
Sample | H1 | H2 | H3 | H4 | H5 | H6 | OH2 | OH3 | OH6 | |
---|---|---|---|---|---|---|---|---|---|---|
NSs | 4.823 | 3.298 | 3.627 | 3.360 | 3.577 | 3.653 | 5.703 | 5.677 | 4.447 | |
NSs–QRC | 4.820 | 3.295 | 3.609 | 3.355 | 3.560 | 3.640 | 5.710 | 5.690 | 4.440 | |
Δδ | 0.003 | 0.003 | 0.018 | 0.005 | 0.017 | 0.013 | −0.010 | −0.013 | −0.007 | |
Sample | H′1 | H′2 | H′3 | H′4 | H′5 | H′6 | H’7 | H’8 | H’9 | H’10 |
QRC | 9.637 | 9.323 | 7.455 | 6.837 | 7.635 | 9.111 | 6.355 | 12.57 | 6.055 | 10.88 |
NSs–QRC | 9.622 | 9.310 | 7.445 | 6.822 | 7.622 | 9.101 | 6.340 | 12.44 | 6.044 | 10.75 |
Δδ | 0.015 | 0.013 | 0.010 | 0.015 | 0.013 | 0.010 | 0.015 | 0.013 | 0.011 | 0.013 |
System | Dh (nm) | ζ-Potential (mV) | PDI |
---|---|---|---|
AgNPs | 31 ± 3 | −33 ± 5 | 0.21 |
Sample | Dh (nm) | ζ-Potentials (mV) | PDI |
---|---|---|---|
NSs | 175 ± 18 | −35 ± 2 | 0.22 |
NS–QRC | 233 ± 5 | −22 ± 3 | 0.33 |
AgNP–NS–QRC | 261 ± 11 | −28 ± 5 | 0.48 |
Concentration (mg/mL) | % Inhibition ± Standard Deviation | ||||
---|---|---|---|---|---|
Sample | |||||
QRC | AgNPs | NS–AgNPs | NS–QRC | AgNP–NS–QRC | |
0.4 | 9.5 ± 2.7 | 10.5 ± 3.2 | 4.7 ± 3.1 | 3.9 ± 0.6 | 9.7 ± 1.7 |
1.2 | 16.2 ± 1.3 | 14.8 ± 1.8 | 8.5 ± 0.9 | 6.7 ± 2.9 | 15.7 ± 0.9 |
2.0 | 20.3 ± 1.2 | 17.4 ± 1.5 | 9.8 ± 0.6 | 39.1 ± 2.2 | 19.0 ± 2.7 |
2.8 | 27.5 ± 0.7 | 21.7 ± 3.2 | 15.2 ± 0.5 | 74.2 ± 0.8 | 26.0 ± 1.8 |
3.6 | 30.8 ± 0.7 | 26.3 ± 1.6 | 19.1 ± 0.8 | 85.0 ± 0.4 | 36.3 ± 1.9 |
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Salazar Sandoval, S.; Bruna, T.; Maldonado-Bravo, F.; Bolaños, K.; Adasme-Reyes, S.; Riveros, A.; Caro, N.; Yutronic, N.; Silva, N.; Kogan, M.J.; et al. β-Cyclodextrin Nanosponges Inclusion Compounds Associated with Silver Nanoparticles to Increase the Antimicrobial Activity of Quercetin. Materials 2023, 16, 3538. https://doi.org/10.3390/ma16093538
Salazar Sandoval S, Bruna T, Maldonado-Bravo F, Bolaños K, Adasme-Reyes S, Riveros A, Caro N, Yutronic N, Silva N, Kogan MJ, et al. β-Cyclodextrin Nanosponges Inclusion Compounds Associated with Silver Nanoparticles to Increase the Antimicrobial Activity of Quercetin. Materials. 2023; 16(9):3538. https://doi.org/10.3390/ma16093538
Chicago/Turabian StyleSalazar Sandoval, Sebastián, Tamara Bruna, Francisca Maldonado-Bravo, Karen Bolaños, Sofía Adasme-Reyes, Ana Riveros, Nelson Caro, Nicolás Yutronic, Nataly Silva, Marcelo J. Kogan, and et al. 2023. "β-Cyclodextrin Nanosponges Inclusion Compounds Associated with Silver Nanoparticles to Increase the Antimicrobial Activity of Quercetin" Materials 16, no. 9: 3538. https://doi.org/10.3390/ma16093538