Next Article in Journal
Utilization of Biomass to Ash: An Overview of the Potential Resources for Alternative Energy
Next Article in Special Issue
Bioactives from Mushroom: Health Attributes and Food Industry Applications
Previous Article in Journal
Increasing the Efficiency of a Spintronic THz Emitter Based on WSe2/FeCo
Previous Article in Special Issue
Natural Cinnamic Acid Derivatives: A Comprehensive Study on Structural, Anti/Pro-Oxidant, and Environmental Impacts
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Materials
Volume 14
Issue 21
10.3390/ma14216480
Review Report
materials-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Effect of Recombinant Human Erythroferrone Protein on Hepcidin Gene (Hamp1) Expression in HepG2 and HuH7 Cells

Materials 2021, 14(21), 6480; https://doi.org/10.3390/ma14216480
by Min Min Than 1,2, Pimpisid Koonyosying 1, Jetsada Ruangsuriya 1, Sunhawit Junrungsee 3, Chairat Uthaipibull 4 and Somdet Srichairatanakool 1,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Materials 2021, 14(21), 6480; https://doi.org/10.3390/ma14216480
Submission received: 30 September 2021 / Revised: 19 October 2021 / Accepted: 25 October 2021 / Published: 28 October 2021
(This article belongs to the Special Issue Characterization, Application and Potential Health Benefits of Bioactive Compounds)

Round 1

Reviewer 1 Report

  1. Hamp1 was descripted in title and abstract, however, hepcidin was wrote in introduction, result, discussion and conclusion sections. Though Hamp1 is hepcidin, authors have to uniform the noun.
  2. Many studies have demonstrated the erythroferrone may influence hepcidin expression. Authors have to introduce more relationships between erythroferrone and hepcidin referred to previous studies.
  3. Authors have to discuss the differences between previous studies and this study about how erythroferrone regulated hepcidin.
  4. Why the ERFE-FLAG have different molecular weight in figure 1? In addition, authors have to mention why the ERFE-FLAG obtained from medium was selected to treat HepG2 and Huhc 7cells.
  5. The figure 4 legend “Relative hepcidin mRNA levels in HepG2 cells at various” was a mistake. Please replace hepG2 with Huhc.

     6. Different effects on ERFE-FLAG-treated HepG2 and Huch 7 cells.        Authors have to discuss the possible reasons.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

The authors presented the influence of recombinant human erythroferrone on expression of hepcidin in hepatocellular carcinoma cell lines HepG2 and Huh7. They found that only in Huh7 cell line ERFE suppresses hepcidin expression what they ascribed to supposed ERFE receptors. In introduction should be I think presented previous knowledge justifying examinations undertook by authors what was not showed clearly. ERFE receptors are only supposed but not demonstrated by authors. Arezes et al. showed that secreted ERFE binds BMP and its receptor and this way inhibits hepcidin expression, may be it was this way in authors experiment. 

Summing up I think in introduction and in discussion should be shown more clearly why authors did the experiments and what comes out of it for the present and future.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Back to TopTop