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Controlled Release of 18-β-Glycyrrhetinic Acid from Core-Shell Nanoparticles: Effects on Cytotoxicity and Intracellular Concentration in HepG2 Cell Line

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Dipartimento di Scienze biotecnologiche di base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy
2
Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy
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National Center for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, Italy
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Department of Chemistry, University La Sapienza, 00185 Rome, Italy
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CIABC-Centro di Ricerca per le Scienze Applicate alla Protezione dell’Ambiente e dei Beni Culturali, University La Sapienza, 00185 Rome, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally.
Academic Editor: Katarzyna Winnicka
Materials 2021, 14(14), 3893; https://doi.org/10.3390/ma14143893
Received: 11 June 2021 / Revised: 2 July 2021 / Accepted: 7 July 2021 / Published: 12 July 2021
18β-glycyrrhetinic acid (GA) is a pentacyclic triterpene with promising hepatoprotective and anti-Hepatocellular carcinoma effects. GA low water solubility however reduces its biodistribution and bioavailability, limiting its applications in biomedicine. In this work we used core-shell NPs made of PolyD-L-lactide-co-glycolide (PLGA) coated with chitosan (CS), prepared through an osmosis-based methodology, to efficiently entrap GA. NPs morphology was investigated with SEM and TEM and their GA payload was evaluated with a spectrophotometric method. GA-loaded NPs were administered to HepG2 cells and their efficiency in reducing cell viability was compared with that induced by the free drug in in vitro tests. Cell viability was evaluated by the MTT assay, as well as with Electric Cells-Substrate Impedance Sensing (ECIS), that provided a real-time continuous monitoring. It was possible to correlate the toxic effect of the different forms of GA with the bioavailability of the drug, evidencing the importance of real-time tests for studying the effects of bioactive substances on cell cultures. View Full-Text
Keywords: nanoparticles; drug delivery; ECIS; cytotoxicity nanoparticles; drug delivery; ECIS; cytotoxicity
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MDPI and ACS Style

Nocca, G.; D’Avenio, G.; Amalfitano, A.; Chronopoulou, L.; Mordente, A.; Palocci, C.; Grigioni, M. Controlled Release of 18-β-Glycyrrhetinic Acid from Core-Shell Nanoparticles: Effects on Cytotoxicity and Intracellular Concentration in HepG2 Cell Line. Materials 2021, 14, 3893. https://doi.org/10.3390/ma14143893

AMA Style

Nocca G, D’Avenio G, Amalfitano A, Chronopoulou L, Mordente A, Palocci C, Grigioni M. Controlled Release of 18-β-Glycyrrhetinic Acid from Core-Shell Nanoparticles: Effects on Cytotoxicity and Intracellular Concentration in HepG2 Cell Line. Materials. 2021; 14(14):3893. https://doi.org/10.3390/ma14143893

Chicago/Turabian Style

Nocca, Giuseppina, Giuseppe D’Avenio, Adriana Amalfitano, Laura Chronopoulou, Alvaro Mordente, Cleofe Palocci, and Mauro Grigioni. 2021. "Controlled Release of 18-β-Glycyrrhetinic Acid from Core-Shell Nanoparticles: Effects on Cytotoxicity and Intracellular Concentration in HepG2 Cell Line" Materials 14, no. 14: 3893. https://doi.org/10.3390/ma14143893

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