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Open AccessArticle

Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells

1
Faculty of Chemistry, Autonomous University of the State of Mexico, Paseo Colon S/N, Toluca 50120, Mexico
2
Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech Republic
3
Faculty of Higher Studies Iztacala, National Autonomous University of Mexico, Los Reyes Iztacala 1, Mexico City 54090, Mexico
*
Authors to whom correspondence should be addressed.
Materials 2021, 14(1), 227; https://doi.org/10.3390/ma14010227
Received: 24 November 2020 / Revised: 21 December 2020 / Accepted: 23 December 2020 / Published: 5 January 2021
This work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed a storage modulus similar to oral mucosa and elastic solid rheological behaviour without sol transition. A comprehensive characterization of porosity by scanning electron microscopy, mercury intrusion porosimetry and nano-computed tomography (with spatial resolution below 1 μm) showed that lyophilisation resulted in the heterogeneous incorporation of closed oval-like pores in the hydrogel with broad size distribution (5 to 180 μm, d50 = 65 μm). Human oral mucosa biopsies were used to isolate hOMSCs, expressing typical markers of mesenchymal stem cells in more than 95% of the cell population. Direct contact cytotoxicity assay demonstrated that PVP hydrogel have no negative effect on cell metabolic activity, allowing the culture of hOMSCs with normal fusiform morphology. Pore connectivity should be improved in future to allow cell growth in the bulk of the PVP hydrogel. View Full-Text
Keywords: polyvinylpyrrolidone; hydrogel; lyophilisation; nano-computed tomography; porosity; oral mucosa; stem cell polyvinylpyrrolidone; hydrogel; lyophilisation; nano-computed tomography; porosity; oral mucosa; stem cell
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MDPI and ACS Style

Oliver-Urrutia, C.; Rosales Ibañez, R.; Flores-Merino, M.V.; Vojtova, L.; Salplachta, J.; Čelko, L.; Kaiser, J.; Montufar, E.B. Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells. Materials 2021, 14, 227. https://doi.org/10.3390/ma14010227

AMA Style

Oliver-Urrutia C, Rosales Ibañez R, Flores-Merino MV, Vojtova L, Salplachta J, Čelko L, Kaiser J, Montufar EB. Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells. Materials. 2021; 14(1):227. https://doi.org/10.3390/ma14010227

Chicago/Turabian Style

Oliver-Urrutia, Carolina; Rosales Ibañez, Raúl; Flores-Merino, Miriam V.; Vojtova, Lucy; Salplachta, Jakub; Čelko, Ladislav; Kaiser, Jozef; Montufar, Edgar B. 2021. "Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells" Materials 14, no. 1: 227. https://doi.org/10.3390/ma14010227

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