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Open AccessArticle

Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry

1
Department of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, Germany
2
Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Strasse 11, 12489 Berlin, Germany
3
SPETEC GmbH, Berghamer Str. 2, 85435 Erding, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Materials 2020, 13(6), 1447; https://doi.org/10.3390/ma13061447
Received: 12 February 2020 / Revised: 10 March 2020 / Accepted: 20 March 2020 / Published: 22 March 2020
(This article belongs to the Special Issue Nanomaterial Characterization Methods: Leaping Towards Validation)
Nano-carrier systems such as liposomes have promising biomedical applications. Nevertheless, characterization of these complex samples is a challenging analytical task. In this study a coupled hydrodynamic chromatography-single particle-inductively coupled plasma mass spectrometry (HDC-spICP-MS) approach was validated based on the technical specification (TS) 19590:2017 of the international organization for standardization (ISO). The TS has been adapted to the hyphenated setup. The quality criteria (QC), e.g., linearity of the calibration, transport efficiency, were investigated. Furthermore, a cross calibration of the particle size was performed with values from dynamic light scattering (DLS) and transmission electron microscopy (TEM). Due to an additional Y-piece, an online-calibration routine was implemented. This approach allows the calibration of the ICP-MS during the dead time of the chromatography run, to reduce the required time and enhance the robustness of the results. The optimized method was tested with different gold nanoparticle (Au-NP) mixtures to investigate the characterization properties of HDC separations for samples with increasing complexity. Additionally, the technique was successfully applied to simultaneously determine both the hydrodynamic radius and the Au-NP content in liposomes. With the established hyphenated setup, it was possible to distinguish between different subpopulations with various NP loads and different hydrodynamic diameters inside the liposome carriers. View Full-Text
Keywords: single particle ICP-MS; nanoparticle characterization; nano-carrier; liposomes; hydrodynamic chromatography (HDC); validation single particle ICP-MS; nanoparticle characterization; nano-carrier; liposomes; hydrodynamic chromatography (HDC); validation
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Hachenberger, Y.U.; Rosenkranz, D.; Kriegel, F.L.; Krause, B.; Matschaß, R.; Reichardt, P.; Tentschert, J.; Laux, P.; Jakubowski, N.; Panne, U.; Luch, A. Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry. Materials 2020, 13, 1447.

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