Bacteria Exposed to Silver Nanoparticles Synthesized by Laser Ablation in Water: Modelling E. coli Growth and Inactivation
by
Lucija Krce 1,*
, Matilda Šprung 2, Ana Maravić 3, Polona Umek 4, Krešimir Salamon 5, Nikša Krstulović 6 and Ivica Aviani 1
Lucija Krce 1,*, Matilda Šprung 2, Ana Maravić 3, Polona Umek 4, Krešimir Salamon 5, Nikša Krstulović 6 and Ivica Aviani 1
1
Faculty of Science, Department of Physics, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia
2
Faculty of Science, Department of Chemistry, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia
3
Faculty of Science, Department of Biology, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia
4
Jožef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia
5
Ruđer Bošković Institute, Bijenička cesta 54, 10 000 Zagreb, Croatia
6
Institute of Physics, Bijenička cesta 46, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Materials 2020, 13(3), 653; https://doi.org/10.3390/ma13030653
Received: 8 January 2020 / Revised: 29 January 2020 / Accepted: 30 January 2020 / Published: 1 February 2020
(This article belongs to the Special Issue Laser Ablation: Materials and Applications)
This study is aimed to better understand the bactericidal mode of action of silver nanoparticles. Here we present the production and characterization of laser-synthesized silver nanoparticles along with growth curves of bacteria treated at sub-minimal and minimal inhibitory concentrations, obtained by optical density measurements. The main effect of the treatment is the increase of the bacterial apparent lag time, which is very well described by the novel growth model as well as the entire growth curves for different concentrations. The main assumption of the model is that the treated bacteria uptake the nanoparticles and inactivate, which results in the decrease of both the nanoparticles and the bacteria concentrations. The lag assumes infinitive value for the minimal inhibitory concentration treatment. This apparent lag phase is not postponed bacterial growth. It is a dynamic state in which the bacterial growth and death rates are close in value. Our results strongly suggest that the predominant mode of antibacterial action of silver nanoparticles is the penetration inside the membrane.
View Full-Text
Keywords:
laser synthesis of nanoparticles; silver nanoparticles; antibacterial activity; modeling bacterial growth
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Krce, L.; Šprung, M.; Maravić, A.; Umek, P.; Salamon, K.; Krstulović, N.; Aviani, I. Bacteria Exposed to Silver Nanoparticles Synthesized by Laser Ablation in Water: Modelling E. coli Growth and Inactivation. Materials 2020, 13, 653.
Show more citation formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
