Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance

Komarova, Ekaterina G.; Sharkeev, Yurii P.; Sedelnikova, Mariya B.; Prymak, Oleg; Epple, Matthias; Litvinova, Larisa S.; Shupletsova, Valeria V.; Malashchenko, Vladimir V.; Yurova, Kristina A.; Dzyuman, Anna N.; Kulagina, Irina V.; Mushtovatova, Lyudmila S.; Bochkareva, Olga P.; Karpova, Mariia R.; Khlusov, Igor A.

doi:10.3390/ma13194366

Open AccessArticle

Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance

by

Ekaterina G. Komarova

^1,*

,

Yurii P. Sharkeev

^1,2

,

Mariya B. Sedelnikova

¹,

Oleg Prymak

³

,

Matthias Epple

³

,

Larisa S. Litvinova

⁴

,

Valeria V. Shupletsova

⁴,

Vladimir V. Malashchenko

⁴,

Kristina A. Yurova

⁴,

Anna N. Dzyuman

⁵,

Irina V. Kulagina

⁶,

Lyudmila S. Mushtovatova

⁷,

Olga P. Bochkareva

⁷,

Mariia R. Karpova

⁷ and

Igor A. Khlusov

^4,5,8

¹

Laboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science SB RAS, 634055 Tomsk, Russia

²

Research School of High-Energy Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia

³

Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45141 Essen, Germany

⁴

Center for Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, 236029 Kaliningrad, Russia

⁵

Department of Morphology and General Pathology, Siberian State Medical University, 634050 Tomsk, Russia

⁶

Department of Biochemistry and Molecular Biology, Siberian State Medical University, 634050 Tomsk, Russia

⁷

Department of Microbiology and Virology, Siberian State Medical University, 634050 Tomsk, Russia

⁸

Research School of Chemistry and Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia

^*

Author to whom correspondence should be addressed.

Materials 2020, 13(19), 4366; https://doi.org/10.3390/ma13194366

Submission received: 4 September 2020 / Revised: 24 September 2020 / Accepted: 27 September 2020 / Published: 30 September 2020

(This article belongs to the Special Issue Recent Advances in Biocoatings)

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Abstract

This work describes the wettability and biological performance of Zn- and Cu-containing CaP-based coatings prepared by micro-arc oxidation on pure titanium (Ti) and novel Ti-40Nb alloy. Good hydrophilic properties of all the coatings were demonstrated by the low contact angles with liquids, not exceeding 45°. An increase in the applied voltage led to an increase of the coating roughness and porosity, thereby reducing the contact angles to 6° with water and to 17° with glycerol. The free surface energy of 75 ± 3 mJ/m² for all the coatings were determined. Polar component was calculated as the main component of surface energy, caused by the presence of strong polar PO₄³⁻ and OH⁻ bonds. In vitro studies showed that low Cu and Zn amounts (~0.4 at.%) in the coatings promoted high motility of human adipose-derived multipotent mesenchymal stromal cells (hAMMSC) on the implant/cell interface and subsequent cell ability to differentiate into osteoblasts. In vivo study demonstrated 100% ectopic bone formation only on the surface of the CaP coating on Ti. The Zn- and Cu-containing CaP coatings on both substrates and the CaP coating on the Ti-40Nb alloy slightly decreased the incidence of ectopic osteogenesis down to 67%. The MAO coatings showed antibacterial efficacy against Staphylococcus aureus and can be arranged as follows: Zn-CaP/Ti > Cu-CaP/TiNb, Zn-CaP/TiNb > Cu-CaP/Ti.

Keywords: micro-arc oxidation; calcium phosphate coating; Ti-40wt.%Nb alloy; wettability; biocompatibility; ectopic bone formation; bone lamellae; antibacterial efficacy

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MDPI and ACS Style

Komarova, E.G.; Sharkeev, Y.P.; Sedelnikova, M.B.; Prymak, O.; Epple, M.; Litvinova, L.S.; Shupletsova, V.V.; Malashchenko, V.V.; Yurova, K.A.; Dzyuman, A.N.; et al. Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance. Materials 2020, 13, 4366. https://doi.org/10.3390/ma13194366

AMA Style

Komarova EG, Sharkeev YP, Sedelnikova MB, Prymak O, Epple M, Litvinova LS, Shupletsova VV, Malashchenko VV, Yurova KA, Dzyuman AN, et al. Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance. Materials. 2020; 13(19):4366. https://doi.org/10.3390/ma13194366

Chicago/Turabian Style

Komarova, Ekaterina G., Yurii P. Sharkeev, Mariya B. Sedelnikova, Oleg Prymak, Matthias Epple, Larisa S. Litvinova, Valeria V. Shupletsova, Vladimir V. Malashchenko, Kristina A. Yurova, Anna N. Dzyuman, and et al. 2020. "Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance" Materials 13, no. 19: 4366. https://doi.org/10.3390/ma13194366

APA Style

Komarova, E. G., Sharkeev, Y. P., Sedelnikova, M. B., Prymak, O., Epple, M., Litvinova, L. S., Shupletsova, V. V., Malashchenko, V. V., Yurova, K. A., Dzyuman, A. N., Kulagina, I. V., Mushtovatova, L. S., Bochkareva, O. P., Karpova, M. R., & Khlusov, I. A. (2020). Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance. Materials, 13(19), 4366. https://doi.org/10.3390/ma13194366

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Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance

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