Cobalt Chromium Molybdenum Surface Modifications Alter the Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. CoCrMo Alloy Surface Modifications
2.2. Scanning Electron Microscopy (SEM)
2.3. Intraoral Tissue Harvest and Cell Culture
2.4. Flow Cytometry
2.5. Multilineage Differentiation Analysis
2.6. Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR)
2.7. xMAP Human Bone Metabolism Magnetic Bead Panel
2.8. Statistical Analysis
3. Results
3.1. Surface Characteristics
3.2. MSPC Characterization and Multilineage Differentiation Analysis
3.3. Expression of Osteogenic Markers on Different CoCrMo Surface Modifications
3.4. The Role of Integrin α5β1 Subunits on Different CoCrMo Surface Modifications
3.5. Expression of Bone Biology Markers on Different CoCrMo Surface Modifications
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene. | Ctrl | Osteogenic Differentiated | ||||
ad Figure 3 | CoCrMo | Polished | Porous | TiN | cpTi | |
Runx2 (Figure 3A) | 1 ± 0.1 | 0.95 ± 0.2 | 0.84 ± 0.3 | 0.96 ± 0.1 | 1.00 ± 0.4 | 0.81 ± 0.3 |
Runx2 (Figure 3B) | 1 ± 0.1 | 1.07 ± 0.2 | 0.77 ± 0.3 | 1.16 ± 0.3 | 0.69 ± 0.2 * | |
ALP (Figure 3A) | 1 ± 0.1 | 5.17 ± 2.4 *** | 5.92 ± 4.1 ** | 5.66 ± 3.3 *** | 3.93 ± 1.8 *** | 7.74 ± 2.8 *** |
ALP (Figure 3B) | 1 ± 0.1 | 1.62 ± 0.4* | 1.26 ± 0.7 | 1.24 ± 0.3 | 1.93 ± 0.5 *** | |
Osteopontin (Figure 3A) | 1 ± 0.1 | 10.63 ± 4.0 *** | 4.38 ± 1.4 *** | 5.73 ± 4.2 * | 8.74 ± 3.2 *** | 9.38 ± 5.6 |
Osteopontin (Figure 3B) | 1 ± 0.1 | 0.68 ± 0.3 * | 0.66 ± 0.2 ** | 1.05 ± 0.5 | 0.84 ± 0.7 | |
Osteonectin (Figure 3A) | 1 ± 0.1 | 1.26 ± 0.3 | 1.33 ± 0.5 | 0.77 ± 0.4 | 1.74 ± 0.4 ** | 1.44 ± 0.3 ** |
Osteonectin (Figure 3B) | 1 ± 0.1 | 1.37 ± 0.5 | 0.79 ± 0.4 | 1.32 ± 0.4 | 1.04 ± 0.3 | |
Ctrl | Osteogenic Differentiated | |||||
ad Figure 4 | CoCrMo | Polished | Porous | TiN | cpTi | |
Integrin α5 (Figure 4A) | 1 ± 0.1 | 5.53 ± 1.2 *** | 6.13 ± 2.0 *** | 4.22 ± 1.8 ** | 6.83 ± 2.0 *** | 7.03 ± 2.5 ** |
Integrin α5 (Figure 4B) | 1 ± 0.1 | 1.41 ± 0.6 | 0.69 ± 0.2 * | 1.35 ± 0.3 * | 2,65 ± 0.8 | |
Integrin β1 (Figure 4A) | 1 ± 0.1 | 1.97 ± 0.3 *** | 1.89 ± 0.6 ** | 1.17 ± 0.2 | 2.25 ± 0.4 *** | 2.16 ± 0.5 ** |
Integrin β1 (Figure 3B) | 1 ± 0.1 | 1.04 ± 0.3 | 0.59 ± 0.11 *** | 1.17 ± 0.3 | 1.07 ± 0.3 |
pg/mL | |||||
---|---|---|---|---|---|
Gene | CoCrMo | Polished | Porous | TiN | cpTi |
OC undiff | 1200 ± 419 | 805 ± 218 | 467 ± 69 | 1195 ± 306 | 999 ± 379 |
OC OG | 2003 ± 591 | 3158 ± 775 * | 1727 ± 447 * | 3117 ± 699 * | 354 ± 184 * |
SM undiff. | n.s. | # | n.s. | n.s. | |
SM OG | n.s. | n.s. | n.s. | ## | |
OPG undiff | 4934 ± 879 | 4279 ± 593 | 6221 ± 840 | 4224 ± 639 | 7674 ± 710 |
OPG OG | 4176 ± 1462 | 4740 ± 1178 | 5985 ± 2565 | 4583 ± 1043 | 6315 ± 2307 |
SM undiff. | n.s. | n.s | n.s. | ## | |
SM OG | n.s. | n.s | n.s. | n.s. | |
SOST undiff | 58.8 ± 21 | 65.1 ± 8 | 73.6 ± 11.8 | 56.2 ± 10.5 | 35.0 ± 7.9 |
SOST OG | 115 ± 7.9 * | 112.5 ± 8.6 *** | 117.1 ± 13.8 * | 107.3 ± 11.3 ** | 116.7 ± 17.6 ** |
SM undiff. | n.s. | # | n.s. | n.s. | |
SM OG | n.s. | n.s. | n.s. | n.s. | |
IL6 undiff | 1957 ± 426 | 2114 ± 575 | 5208 ± 1230 | 2099 ± 324 | 4857 ± 1556 |
IL6 OG | 187 ± 128 *** | 262 ± 57 * | 218 ± 76 * | 295 ± 91 ** | 435 ± 296 * |
SM undiff. | n.s. | # | n.s. | # | |
SM OG | n.s. | n.s. | n.s. | n.s. | |
Leptin undiff | 27.3 ± 4.5 | 17.7 ± 4.6 | 267 ± 107 | 33 ± 12.3 | 313 ± 175 |
Leptin OG | 3642 ± 1877 *** | 1478 ± 405 ** | 2230 ± 811 ** | 2498 ± 549 *** | 14911 ± 4053 *** |
SM undiff. | # | # | n.s. | ## | |
SM OG | n.s. | n.s. | n.s. | ## | |
DKK undiff | 371 ± 196 | 311 ± 79 | 1978 ± 737 | 266 ± 106 | 659 ± 232 |
DKK OG | 4410 ± 468 *** | 4179 ± 449 *** | 3816 ± 1482 | 4350 ± 641 *** | 5557 ± 1266 ** |
SM undiff. | n.s. | # | n.s. | n.s. | |
SM OG | n.s. | n.s. | n.s. | n.s. |
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Lohberger, B.; Eck, N.; Glaenzer, D.; Lichtenegger, H.; Ploszczanski, L.; Leithner, A. Cobalt Chromium Molybdenum Surface Modifications Alter the Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells. Materials 2020, 13, 4292. https://doi.org/10.3390/ma13194292
Lohberger B, Eck N, Glaenzer D, Lichtenegger H, Ploszczanski L, Leithner A. Cobalt Chromium Molybdenum Surface Modifications Alter the Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells. Materials. 2020; 13(19):4292. https://doi.org/10.3390/ma13194292
Chicago/Turabian StyleLohberger, Birgit, Nicole Eck, Dietmar Glaenzer, Helga Lichtenegger, Leon Ploszczanski, and Andreas Leithner. 2020. "Cobalt Chromium Molybdenum Surface Modifications Alter the Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells" Materials 13, no. 19: 4292. https://doi.org/10.3390/ma13194292