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In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging

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Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan
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Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan
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Department of Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, 465 Kajii-cho, Kyoto 602-8566, Japan
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Department of Infectious Diseases, Kyoto Prefectural University of Medicine, Kamigyo-ku, 465 Kajii-cho, Kyoto 602-8566, Japan
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Department of Chemical Engineering and Materials Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe-shi, Kyoto-fu 610-0394, Japan
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Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
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Division of Oral Biology and Medicine, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, Los Angeles, CA 90095, USA
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Division of Advanced Prosthodontics, The Jane and Jerry Weintraub Center for Re-constructive Biotechnology, UCLA School of Dentistry, Los Angeles, CA 90095, USA
*
Authors to whom correspondence should be addressed.
Materials 2020, 13(19), 4275; https://doi.org/10.3390/ma13194275
Received: 31 August 2020 / Revised: 23 September 2020 / Accepted: 23 September 2020 / Published: 25 September 2020
(This article belongs to the Special Issue Advances in Dental Bio-Nanomaterials)
The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects. View Full-Text
Keywords: spectroscopic imaging; nanogel; scaffold; bone tissue regeneration; Raman spectroscopy spectroscopic imaging; nanogel; scaffold; bone tissue regeneration; Raman spectroscopy
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MDPI and ACS Style

Adachi, T.; Boschetto, F.; Miyamoto, N.; Yamamoto, T.; Marin, E.; Zhu, W.; Kanamura, N.; Tahara, Y.; Akiyoshi, K.; Mazda, O.; Nishimura, I.; Pezzotti, G. In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging. Materials 2020, 13, 4275. https://doi.org/10.3390/ma13194275

AMA Style

Adachi T, Boschetto F, Miyamoto N, Yamamoto T, Marin E, Zhu W, Kanamura N, Tahara Y, Akiyoshi K, Mazda O, Nishimura I, Pezzotti G. In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging. Materials. 2020; 13(19):4275. https://doi.org/10.3390/ma13194275

Chicago/Turabian Style

Adachi, Tetsuya, Francesco Boschetto, Nao Miyamoto, Toshiro Yamamoto, Elia Marin, Wenliang Zhu, Narisato Kanamura, Yoshiro Tahara, Kazunari Akiyoshi, Osam Mazda, Ichiro Nishimura, and Giuseppe Pezzotti. 2020. "In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging" Materials 13, no. 19: 4275. https://doi.org/10.3390/ma13194275

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