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Article

Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects

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Matriflex LLC, 12 Aviakonstruktora Mikoyana Street, Building A, 2nd Floor, Office 1, Moscow 125252, Russia
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Department of Immunology, Gamaleya National Research Centre of Epidemiology and Microbiology, 18 Gamaleya Street, Moscow 123098, Russia
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Faculty of Biology, Moscow State University, 73A, Leninskie Gory Street, Moscow 119330, Russia
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Laboratory for Vascular Biology, Department of Experimental Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, 6, Sosnovy Boulevard, Kemerovo 650002, Russia
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Author to whom correspondence should be addressed.
Materials 2020, 13(15), 3393; https://doi.org/10.3390/ma13153393
Received: 1 July 2020 / Revised: 27 July 2020 / Accepted: 30 July 2020 / Published: 31 July 2020
(This article belongs to the Special Issue Novel Grafts and Biomaterials in Bone Tissue Engineering)
Here we evaluated the efficacy of bone repair using various native bovine biomaterials (refined hydroxyapatite (HA), demineralised bone matrix (DBM), and purified bone collagen (COLL)) as compared with commercially available bone mineral and bone autografts. We employed a conventional critical-sized (8 mm diameter) rat calvarial defect model (6-month-old male Sprague–Dawley rats, n = 72 in total). The artificial defect was repaired using HA, DBM, COLL, commercially available bone mineral powder, bone calvarial autograft, or remained unfilled (n = 12 animals per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 6 per time point) with the subsequent examination to assess the extent, volume, area, and mineral density of the repaired tissue by means of microcomputed tomography and hematoxylin and eosin staining. Bovine HA and DBM powder exhibited excellent repair capability similar to the autografts and commercially available bone mineral powder while COLL showed higher bone repair rate. We suggest that HA and DBM powder obtained from bovine bone tissue can be equally applied for the repair of bone defects and demonstrate sufficient potential to be implemented into clinical studies. View Full-Text
Keywords: bone defects; bone repair; bone autografts; xenogeneic implants; hydroxyapatite; demineralised bone matrix; bone collagen; critical-sized rat calvarial defect; microcomputed tomography; clinical translation bone defects; bone repair; bone autografts; xenogeneic implants; hydroxyapatite; demineralised bone matrix; bone collagen; critical-sized rat calvarial defect; microcomputed tomography; clinical translation
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MDPI and ACS Style

Veremeev, A.; Bolgarin, R.; Nesterenko, V.; Andreev-Andrievskiy, A.; Kutikhin, A. Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects. Materials 2020, 13, 3393. https://doi.org/10.3390/ma13153393

AMA Style

Veremeev A, Bolgarin R, Nesterenko V, Andreev-Andrievskiy A, Kutikhin A. Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects. Materials. 2020; 13(15):3393. https://doi.org/10.3390/ma13153393

Chicago/Turabian Style

Veremeev, Alexey, Roman Bolgarin, Vladimir Nesterenko, Alexander Andreev-Andrievskiy, and Anton Kutikhin. 2020. "Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects" Materials 13, no. 15: 3393. https://doi.org/10.3390/ma13153393

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