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Open AccessArticle

Low-Cost Method and Biochip for Measuring the Trans-Epithelial Electrical Resistance (TEER) of Esophageal Epithelium

1
VALENS-Centre for Bio Devices and Signal Analysis, EEE School, Nanyang Technological University, Blk S1, Level B4, Unit 06 (S1-B4b-06), 50 Nanyang Avenue, Singapore 639798, Singapore
2
Histopathology Department, Singapore General Hospital (SGH), 20 College Road, Academia, Level 10, Diagnostic Tower, Singapore 169856, Singapore
*
Author to whom correspondence should be addressed.
Materials 2020, 13(10), 2354; https://doi.org/10.3390/ma13102354
Received: 18 April 2020 / Revised: 13 May 2020 / Accepted: 18 May 2020 / Published: 20 May 2020
(This article belongs to the Special Issue Advanced Designs of Materials, Devices and Techniques for Biosensing)
Trans-epithelial electrical resistance (TEER) is a good indicator of the barrier integrity of epithelial tissues and is often employed in biomedical research as an effective tool to assess ion transport and permeability of tight junctions. The Ussing chamber is the gold standard for measuring TEER of tissue specimens, but it has major drawbacks: it is a macroscopic method that requires a careful and labor intensive sample mounting protocol, allows a very limited viability for the mounted sample, has large parasitic components and low throughput as it cannot perform multiple simultaneous measurements, and this sophisticated and delicate apparatus has a relatively high cost. This paper demonstrates a low-cost home-made “sandwich ring” method which was used to measure the TEER of tissue specimens effectively. This method inspired the subsequent design of a biochip fabricated using standard soft lithography and laser engraving technologies, with which the TEER of pig epithelial tissues was measured. Moreover, it was possible to temporarily preserve the tissue specimens for days in the biochip and monitor the TEER continuously. Tissue responses after exposure tests to media of various pH values were also successfully recorded using the biochip. All these demonstrate that this biochip could be an effective, cheaper, and easier to use Ussing chamber substitute that may have relevant applications in clinical practice. View Full-Text
Keywords: TEER measurement; tissue biochip; tissue viability; esophageal epithelial integrity; Lab-on-a-Chip; Ussing chamber TEER measurement; tissue biochip; tissue viability; esophageal epithelial integrity; Lab-on-a-Chip; Ussing chamber
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MDPI and ACS Style

Poenar, D.P.; Yang, G.; Wan, W.K.; Feng, S. Low-Cost Method and Biochip for Measuring the Trans-Epithelial Electrical Resistance (TEER) of Esophageal Epithelium. Materials 2020, 13, 2354.

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