In Situ Controlled Surface Microstructure of 3D Printed Ti Alloy to Promote Its Osteointegration
Abstract
:1. Introduction
2. Materials and Methods
2.1. 3D Printing of Ti Alloy Implants
2.2. Hydrothermal Treatment
2.3. Characterization of Prepared Ti Alloy Implants
2.4. Protein Adsorption
2.5. Cell Culture
2.6. Cell Viability
2.7. Cell Differentiation
2.8. Cellular Cytoskeleton Morphology
2.9. Statistical Analysis
3. Results
3.1. The In Situ Tailored Surface Structures of 3D-Printed Ti Alloy Implants
3.2. The Post Hydrothermal Treatment of the Tailored Surface Structures
3.3. Surface Composition
3.4. In-Vitro Evaluation
3.4.1. Protein Adsorption
3.4.2. Cell Viability
3.4.3. Cell Distribution on Various Surfaces
3.4.4. Cell Differentiation
4. Discussion
4.1. In Situ, Controlled Surface Structure
4.1.1. In Situ, Controlled Surface Microstructure
4.1.2. Construction of Nanostructure
4.2. Analysis of Cellular Responses on the Tailored Surface Structures of the Implants
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Area | Ti K | Al K | V K | O K |
---|---|---|---|---|
Substrate | 73.98 | 5.51 | 3.28 | 14.86 |
Microsphere | 72.44 | 5.46 | 3.16 | 15.87 |
Graininess | 35.22 | 16.00 | 2.01 | 46.77 |
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Shan, L.; Kadhum, A.A.H.; Al-Furjan, M.S.H.; Weng, W.; Gong, Y.; Cheng, K.; Zhou, M.; Dong, L.; Chen, G.; Takriff, M.S.; et al. In Situ Controlled Surface Microstructure of 3D Printed Ti Alloy to Promote Its Osteointegration. Materials 2019, 12, 815. https://doi.org/10.3390/ma12050815
Shan L, Kadhum AAH, Al-Furjan MSH, Weng W, Gong Y, Cheng K, Zhou M, Dong L, Chen G, Takriff MS, et al. In Situ Controlled Surface Microstructure of 3D Printed Ti Alloy to Promote Its Osteointegration. Materials. 2019; 12(5):815. https://doi.org/10.3390/ma12050815
Chicago/Turabian StyleShan, Lijun, Abdul Amir H. Kadhum, M.S.H. Al-Furjan, Wenjian Weng, Youping Gong, Kui Cheng, Maoying Zhou, Lingqing Dong, Guojin Chen, Mohd S. Takriff, and et al. 2019. "In Situ Controlled Surface Microstructure of 3D Printed Ti Alloy to Promote Its Osteointegration" Materials 12, no. 5: 815. https://doi.org/10.3390/ma12050815