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Open AccessArticle

General Study and Gene Expression Profiling of Endotheliocytes Cultivated on Electrospun Materials

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Laboratory of Biomedical Technologies, Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Rechkunovskaya str. 15, 630055 Novosibirsk, Russia
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Laboratory of Molecular Medicine, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), Lavrentiev ave. 8, 630090 Novosibirsk, Russia
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Department of the Regulation of Genetic Processes, Laboratory of Genomics, Institute of Molecular and Cell Biology, Siberian Branch of the Russian Academy of Sciences (IMCB SB RAS), Lavrentiev ave. 8/2, 630090 Novosibirsk, Russia
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Department of Natural Sciences, Epigenetics Laboratory, Novosibirsk State University, Pirogova str. 2, 630090 Novosibirsk, Russia
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Laboratory of Biomedical Chemistry, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), Lavrentiev ave. 8, 630090 Novosibirsk, Russia
*
Author to whom correspondence should be addressed.
Materials 2019, 12(24), 4082; https://doi.org/10.3390/ma12244082
Received: 24 October 2019 / Revised: 20 November 2019 / Accepted: 3 December 2019 / Published: 6 December 2019
(This article belongs to the Section Biomaterials)
Endothelization of the luminal surface of vascular grafts is required for their long-term functioning. Here, we have cultivated human endothelial cells (HUVEC) on different 3D matrices to assess cell proliferation, gene expression and select the best substrate for endothelization. 3D matrices were produced by electrospinning from solutions of poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL), and blends of PCL with gelatin (Gl) in hexafluoroisopropanol. Structure and surface properties of 3D matrices were characterized by SEM, AFM, and sessile drop analysis. Cell adhesion, viability, and proliferation were studied by SEM, Alamar Blue staining, and 5-ethynyl-2’-deoxyuridine (EdU) assay. Gene expression profiling was done on an Illumina HiSeq 2500 platform. Obtained data indicated that 3D matrices produced from PCL with Gl and treated with glutaraldehyde provide the most suitable support for HUVEC adhesion and proliferation. Transcriptome sequencing has demonstrated a minimal difference of gene expression profile in HUVEC cultivated on the surface of these matrices as compared to tissue culture plastic, thus confirming these matrices as the best support for endothelization. View Full-Text
Keywords: electrospinning; 3D matrices; polycaprolactone; vascular grafts; endothelization; RNA sequencing electrospinning; 3D matrices; polycaprolactone; vascular grafts; endothelization; RNA sequencing
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Stepanova, A.O.; Laktionov, P.P.; Cherepanova, A.V.; Chernonosova, V.S.; Shevelev, G.Y.; Zaporozhchenko, I.A.; Karaskov, A.M.; Laktionov, P.P. General Study and Gene Expression Profiling of Endotheliocytes Cultivated on Electrospun Materials. Materials 2019, 12, 4082.

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