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Open AccessArticle

Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering

1
Department for Oral, Cranio-Maxillofacial, and Facial Plastic Surgery, Frankfurt Orofacial Regenerative Medicine (FORM) Lab, University Hospital Frankfurt Goethe University, 60590 Frankfurt am Main, Germany
2
Department of Orthodontics and Orofacial Orthopedics, University of Erlangen, 91052 Erlangen, Germany
3
Department of Periodontology, University of Bern, 3004 Bern, Switzerland
4
Private practice, Pain Therapy Center, 06100 Nice, France
*
Author to whom correspondence should be addressed.
Materials 2019, 12(23), 3993; https://doi.org/10.3390/ma12233993
Received: 11 June 2019 / Revised: 4 November 2019 / Accepted: 27 November 2019 / Published: 2 December 2019
(This article belongs to the Special Issue Advances in Platelet-rich Fibrin for Bone Tissue Engineering)
Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on the previously described low-speed centrifuge concept (LSCC), which allowed the introduction of a liquid-PRF formulation of fibrinogen and thrombin prior to its conversion to fibrin. Liquid-PRF was introduced to meet the clinical demand for combination with biomaterials in a clinically applicable and easy-to-use way. The aim of the present study was to evaluate, ex vivo, the interaction of the liquid-PRF constituents with five different collagen biomaterials by histological analyses. The results first demonstrated that large variability existed between the biomaterials investigated. Liquid-PRF was able to completely invade Mucograft® (MG; Geistlich Biomaterials, Wolhusen, Switzerland) and to partly invade Bio-Gide® (BG; Geistlich Biomaterials, Wolhusen, Switzerland) and Mucoderm® (MD; Botiss Biomaterials, Berlin, Germany), and Collprotect® (CP; Botiss Biomaterials, Berlin, Germany) showed only a superficial interaction. The BEGO® collagen membrane (BCM; BEGO Implant Systems) appeared to be completely free of liquid-PRF. These results were confirmed by the different cellular penetration and liquid-PRF absorption coefficient (PAC) values of the evaluated membranes. The present study demonstrates a system for loading biomaterials with a complex autologous cell system (liquid-PRF) in a relatively short period of time and in a clinically relevant manner. The combination of biomaterials with liquid-PRF may be clinically utilized to enhance the bioactivity of collagen-based biomaterials and may act as a biomaterial-based growth factor delivery system. View Full-Text
Keywords: collagen; leukocytes; platelets; platelet-rich fibrin; tissue engineering; centrifugation; liquid-PRF; LSCC collagen; leukocytes; platelets; platelet-rich fibrin; tissue engineering; centrifugation; liquid-PRF; LSCC
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MDPI and ACS Style

Al-Maawi, S.; Herrera-Vizcaíno, C.; Orlowska, A.; Willershausen, I.; Sader, R.; Miron, R.J.; Choukroun, J.; Ghanaati, S. Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering. Materials 2019, 12, 3993.

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