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Open AccessArticle

Development of Oxidized Polyvinyl Alcohol-Based Nerve Conduits Coupled with the Ciliary Neurotrophic Factor

1
Department of Neurosciences, Section of Human Anatomy, University of Padova, Via A. Gabelli 65, 35121 Padova, Italy
2
L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via N. Giustiniani 2, 35128 Padova, Italy
3
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35128 Padova, Italy
4
Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling (T.E.S.) Onlus, 35030 Padua, Italy
*
Author to whom correspondence should be addressed.
These Authors contributed equally to this work.
Materials 2019, 12(12), 1996; https://doi.org/10.3390/ma12121996
Received: 26 April 2019 / Revised: 9 June 2019 / Accepted: 19 June 2019 / Published: 21 June 2019
Functionalized synthetic conduits represent a promising strategy to enhance peripheral nerve regeneration by guiding axon growth while delivering therapeutic neurotrophic factors. In this work, hollow nerve conduits made of polyvinyl alcohol partially oxidized with bromine (OxPVA_Br2) and potassium permanganate (OxPVA_KMnO4) were investigated for their structural/biological properties and ability to absorb/release the ciliary neurotrophic factor (CNTF). Chemical oxidation enhanced water uptake capacity of the polymer, with maximum swelling index of 60.5% ± 2.5%, 71.3% ± 3.6% and 19.5% ± 4.0% for OxPVA_Br2, OxPVA_KMnO4 and PVA, respectively. Accordingly, hydrogel porosity increased from 15.27% ± 1.16% (PVA) to 62.71% ± 8.63% (OxPVA_Br2) or 77.50% ± 3.39% (OxPVA_KMnO4) after oxidation. Besides proving that oxidized PVA conduits exhibited mechanical resistance and a suture holding ability, they did not exert a cytotoxic effect on SH-SY5Y and Schwann cells and biodegraded over time when subjected to enzymatic digestion, functionalization with CNTF was performed. Interestingly, higher amounts of neurotrophic factor were detected in the lumen of OxPVA_Br2 (0.22 ± 0.029 µg) and OxPVA_KMnO4 (0.29 ± 0.033 µg) guides rather than PVA (0.11 ± 0.021 µg) tubular scaffolds. In conclusion, we defined a promising technology to obtain drug delivery conduits based on functionalizable oxidized PVA hydrogels. View Full-Text
Keywords: nerve conduits; polyvinyl alcohol; chemical oxidation; ciliary neurotrophic factor; functionalization; drug delivery; bioactive scaffolds; peripheral nerve injury; neural regeneration nerve conduits; polyvinyl alcohol; chemical oxidation; ciliary neurotrophic factor; functionalization; drug delivery; bioactive scaffolds; peripheral nerve injury; neural regeneration
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MDPI and ACS Style

Porzionato, A.; Barbon, S.; Stocco, E.; Dalzoppo, D.; Contran, M.; De Rose, E.; Parnigotto, P.P.; Macchi, V.; Grandi, C.; De Caro, R. Development of Oxidized Polyvinyl Alcohol-Based Nerve Conduits Coupled with the Ciliary Neurotrophic Factor. Materials 2019, 12, 1996.

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