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Macroporous Polymer–Protein Hybrid Materials for Antibody Purification by Combination of Reactive Gelation and Click-Chemistry

Department of Chemistry and Applied Biosciences, Institute of Chemical and Bioengineering, ETH Zurich, 8093 Zurich, Switzerland
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Materials 2019, 12(10), 1580; https://doi.org/10.3390/ma12101580
Received: 10 April 2019 / Revised: 3 May 2019 / Accepted: 13 May 2019 / Published: 14 May 2019
(This article belongs to the Section Porous Materials)
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Abstract

Clickable core-shell nanoparticles based on poly(styrene-co-divinylbenzene-co-vinylbenzylazide) have been synthesized via emulsion polymerization. The 38 nm sized particles have been swollen by divinyl benzene (DVB) and 2,2’-azobis(2-methylpropionitrile) (AIBN) and subsequently processed under high shear rates in a Z-shaped microchannel giving macroporous microclusters (100 µm), through the reactive gelation process. The obtained clusters were post-functionalized by “click-chemistry” with propargyl-PEG-NHS-ester and propargylglicidyl ether, yielding epoxide or NHS-ester activated polymer supports for bioconjugation. Macroporous affinity materials for antibody capturing were produced by immobilizing recombinant Staphylococcus aureus protein A on the polymeric support. Coupling chemistry exploiting thiol-epoxide ring-opening reactions with cysteine-containing protein A revealed up to three times higher binding capacities compared to the protein without cysteine. Despite the lower binding capacities compared to commercial affinity phases, the produced polymer–protein hybrids can serve as stationary phases for immunoglobulin affinity chromatography as the materials revealed superior intra-particle mass transports. View Full-Text
Keywords: macroporous Materials; click-chemistry; reactive gelation; protein immobilization; antibody purification macroporous Materials; click-chemistry; reactive gelation; protein immobilization; antibody purification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Lorenz, M.; Paganini, C.; Storti, G.; Morbidelli, M. Macroporous Polymer–Protein Hybrid Materials for Antibody Purification by Combination of Reactive Gelation and Click-Chemistry. Materials 2019, 12, 1580.

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