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Article

A Novel Fluorescent Biosensor for Adenosine Triphosphate Detection Based on a Metal–Organic Framework Coating Polydopamine Layer

by 1,* and 2
1
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
2
School of Materials Science and Engineering, PCFM Lab, Sun Yat-sen University, Guangzhou 510275, China
*
Author to whom correspondence should be addressed.
Materials 2018, 11(9), 1616; https://doi.org/10.3390/ma11091616
Received: 3 August 2018 / Revised: 24 August 2018 / Accepted: 24 August 2018 / Published: 5 September 2018
(This article belongs to the Special Issue Damage Detection and Characterization of High Performance Composites)
In this work, a novel and sensitive fluorescent biosensor based on polydopamine coated Zr-based metal–organic framework (PDA/UiO-66) is presented for adenosine triphosphate (ATP) detection. This PDA/UiO-66 nanoparticle which holds a great potential to be excellent fluorescence quencher can protect the 6-carboxyfluorescein (FAM)-labeled probe from cleaved by DNase I dispersed in solution and the flurescence of labeled FAM is quenched. When ATP molecules exist, aptamers on the PDA/UiO-66 nanoparticles can hybridize with ATP molecule to form complex structure that will be desorbed from the PDA/UiO-66 and digested by DNase I. After that, the released ATP molecule can react with another aptamer on the PDA/UiO-66 complexes, then restarts a new cycle. Herein, the excellent strong fluorescence quenching ability and uploading more amount of aptamer probes of PDA/UiO-66 composites make them efficient biosensors, leading to a high sensitivity with detection limit of 35 nM. Compared with ATP detection directly by UiO-66-based method, the LOD is about 5.7 times higher with PDA/UiO-66 nanoparticle. Moreover, the enhanced biocompatibility and bioactivity with PDA layer of the composites render a proposed strategy for clinical diagnosis field of detecting small biological molecules in vivo in the future. View Full-Text
Keywords: UiO-66; ATP detection; polydopamine layer; fluorescence assay UiO-66; ATP detection; polydopamine layer; fluorescence assay
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MDPI and ACS Style

Xu, P.; Liao, G. A Novel Fluorescent Biosensor for Adenosine Triphosphate Detection Based on a Metal–Organic Framework Coating Polydopamine Layer. Materials 2018, 11, 1616. https://doi.org/10.3390/ma11091616

AMA Style

Xu P, Liao G. A Novel Fluorescent Biosensor for Adenosine Triphosphate Detection Based on a Metal–Organic Framework Coating Polydopamine Layer. Materials. 2018; 11(9):1616. https://doi.org/10.3390/ma11091616

Chicago/Turabian Style

Xu, Peipei, and Guangfu Liao. 2018. "A Novel Fluorescent Biosensor for Adenosine Triphosphate Detection Based on a Metal–Organic Framework Coating Polydopamine Layer" Materials 11, no. 9: 1616. https://doi.org/10.3390/ma11091616

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