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Layer-by-Layer Heparinization of the Cell Surface by Using Heparin-Binding Peptide Functionalized Human Serum Albumin

College of Life and Health Sciences, Northeastern University, Shenyang, 110169, China
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Materials 2018, 11(5), 849; https://doi.org/10.3390/ma11050849
Received: 27 April 2018 / Revised: 17 May 2018 / Accepted: 18 May 2018 / Published: 20 May 2018
(This article belongs to the Section Biomaterials)
Layer-by-layer heparinization of therapeutic cells prior to transplantation is an effective way to inhibit the instant blood-mediated inflammatory reactions (IBMIRs), which are the major cause of early cell graft loss during post-transplantation. Here, a conjugate of heparin-binding peptide (HBP) and human serum albumin (HSA), HBP-HSA, was synthesized by using heterobifunctional crosslinker. After the first heparin layer was coated on human umbilical vein endothelial cells (HUVECs) by means of the HBP-polyethylene glycol-phospholipid conjugate, HBP-HSA and heparin were then applied to the cell surface sequentially to form multiple layers. The immobilization and retention of heparin were analyzed by confocal microscopy and flow cytometry, respectively, and the cytotoxity of HBP-HSA was further evaluated by cell viability assay. Results indicated that heparin was successfully introduced to the cell surface in a layer-by-layer way and retained for at least 24 h, while the cytotoxity of HBP-HSA was negligible at the working concentration. Accordingly, this conjugate provides a promising method for co-immobilization of heparin and HSA to the cell surface under physiological conditions with improved biocompatibility. View Full-Text
Keywords: cell surface modification; heparin-binding peptide; heparinization; layer-by-layer; human serum albumin cell surface modification; heparin-binding peptide; heparinization; layer-by-layer; human serum albumin
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Song, G.; Hu, Y.; Liu, Y.; Jiang, R. Layer-by-Layer Heparinization of the Cell Surface by Using Heparin-Binding Peptide Functionalized Human Serum Albumin. Materials 2018, 11, 849.

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