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Open AccessArticle

The Role of Mucin in the Toxicological Impact of Polystyrene Nanoparticles

1
Department of Medical Chemistry, Medical University of Gdansk, 80-211 Gdańsk, Poland
2
School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, the University of Dublin, Dublin 2, Ireland
3
Center for Research on Adaptive Nanostructures and Nanodevices, Trinity College Dublin, Dublin 2, Ireland
4
College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada
5
Trinity Biomedical Sciences Institute, Trinity College Dublin, the University of Dublin, Dublin 2, Ireland
6
School of Medicine, Trinity College Dublin, the University of Dublin, Dublin 2, Ireland
*
Author to whom correspondence should be addressed.
Materials 2018, 11(5), 724; https://doi.org/10.3390/ma11050724
Received: 10 April 2018 / Revised: 19 April 2018 / Accepted: 1 May 2018 / Published: 3 May 2018
(This article belongs to the Special Issue Nanomaterials for Biomedical Applications)
The development of novel oral drug delivery systems is an expanding area of research and both new approaches for improving their efficacy and the investigation of their potential toxicological effect are crucial and should be performed in parallel. Polystyrene nanoparticles (NPs) have been used for the production of diagnostic and therapeutic nanosystems, are widely used in food packaging, and have also served as models for investigating NPs interactions with biological systems. The mucous gel layer that covers the epithelium of the gastrointestinal system is a complex barrier-exchange system that it is mainly constituted by mucin and it constitutes the first physical barrier encountered after ingestion. In this study, we aimed to investigate the effect of polystyrene NPs on mucin and its potential role during NP–cell interactions. For this purpose, we evaluated the interaction of polystyrene NPs with mucin in dispersion by dynamic light scattering and with a deposited layer of mucin using a quartz crystal microbalance with dissipation technology. Next, we measured cell viability and the apoptotic state of three enterocyte-like cell lines that differ in their ability to produce mucin, after their exposure to the NPs. Positive charged NPs showed the ability to strongly interact and aggregate mucin in our model. Positive NPs affected cell viability and induced apoptosis in all cell lines independently of their ability of produce mucin. View Full-Text
Keywords: polystyrene; nanoparticles; mucin; DLS; QCM-D; cytotoxicity polystyrene; nanoparticles; mucin; DLS; QCM-D; cytotoxicity
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Inkielewicz-Stepniak, I.; Tajber, L.; Behan, G.; Zhang, H.; Radomski, M.W.; Medina, C.; Santos-Martinez, M.J. The Role of Mucin in the Toxicological Impact of Polystyrene Nanoparticles. Materials 2018, 11, 724.

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