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Open AccessArticle

The Potential of Using Immobilized Xylanases to Enhance the Hydrolysis of Soluble, Biomass Derived Xylooligomers

1
Forest Products Biotechnology/Bioenergy Group, Department of Wood Science, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada
2
Department of Chemical and Petroleum Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4, Canada
3
S2G BioChemicals, 4250 Wesbrook Mall, Vancouver, BC V6T 1W5, Canada
*
Author to whom correspondence should be addressed.
Materials 2018, 11(10), 2005; https://doi.org/10.3390/ma11102005
Received: 4 July 2018 / Revised: 19 September 2018 / Accepted: 3 October 2018 / Published: 17 October 2018
Earlier work had indicated that enzyme-mediated hydrolysis of xylooligomer-rich water-soluble streams (derived from steam pre-treated wheat straw) resulted in the effective production of xylose which was subsequently used to produce bio-glycol. In the work reported here, both the thermostability and recyclability of xylanases were significantly improved by covalent immobilizing the enzymes onto alginate beads. The immobilized xylanases showed a lower hydrolytic potential (~55% xylooligomer conversion) compared to the commercial xylanase cocktail HTec3 (~90% xylooligomer conversion) when used at the same protein loading concentration. This was likely due to the less efficient immobilization of key higher molecular weight enzymes (>75 kDa), such as β-xylosidases. However, enzyme immobilization could be improved by lowering the glutaraldehyde loading used to activate the alginate beads, resulting in improved hydrolysis efficacy (~65% xylooligomer conversion). Enzyme immobilization improved enzyme thermostability (endoxylanase and β-xylosidase activities were improved by 80% and 40%, respectively, after 24 h hydrolysis) and this allowed the immobilized enzymes to be reused/recycled for multiple rounds of hydrolysis (up to five times) without any significant reduction in their hydrolytic potential. View Full-Text
Keywords: biorefining; xylanases; enzyme immobilization; biomass pre-treatment; xylooligomers biorefining; xylanases; enzyme immobilization; biomass pre-treatment; xylooligomers
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Hu, J.; Davies, J.; Mok, Y.K.; Arato, C.; Saddler, J.N. The Potential of Using Immobilized Xylanases to Enhance the Hydrolysis of Soluble, Biomass Derived Xylooligomers. Materials 2018, 11, 2005.

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