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Open AccessArticle

The Potential of Using Immobilized Xylanases to Enhance the Hydrolysis of Soluble, Biomass Derived Xylooligomers

Forest Products Biotechnology/Bioenergy Group, Department of Wood Science, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada
Department of Chemical and Petroleum Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4, Canada
S2G BioChemicals, 4250 Wesbrook Mall, Vancouver, BC V6T 1W5, Canada
Author to whom correspondence should be addressed.
Materials 2018, 11(10), 2005;
Received: 4 July 2018 / Revised: 19 September 2018 / Accepted: 3 October 2018 / Published: 17 October 2018
Earlier work had indicated that enzyme-mediated hydrolysis of xylooligomer-rich water-soluble streams (derived from steam pre-treated wheat straw) resulted in the effective production of xylose which was subsequently used to produce bio-glycol. In the work reported here, both the thermostability and recyclability of xylanases were significantly improved by covalent immobilizing the enzymes onto alginate beads. The immobilized xylanases showed a lower hydrolytic potential (~55% xylooligomer conversion) compared to the commercial xylanase cocktail HTec3 (~90% xylooligomer conversion) when used at the same protein loading concentration. This was likely due to the less efficient immobilization of key higher molecular weight enzymes (>75 kDa), such as β-xylosidases. However, enzyme immobilization could be improved by lowering the glutaraldehyde loading used to activate the alginate beads, resulting in improved hydrolysis efficacy (~65% xylooligomer conversion). Enzyme immobilization improved enzyme thermostability (endoxylanase and β-xylosidase activities were improved by 80% and 40%, respectively, after 24 h hydrolysis) and this allowed the immobilized enzymes to be reused/recycled for multiple rounds of hydrolysis (up to five times) without any significant reduction in their hydrolytic potential. View Full-Text
Keywords: biorefining; xylanases; enzyme immobilization; biomass pre-treatment; xylooligomers biorefining; xylanases; enzyme immobilization; biomass pre-treatment; xylooligomers
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Hu, J.; Davies, J.; Mok, Y.K.; Arato, C.; Saddler, J.N. The Potential of Using Immobilized Xylanases to Enhance the Hydrolysis of Soluble, Biomass Derived Xylooligomers. Materials 2018, 11, 2005.

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