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Open AccessCommunication
Energies 2015, 8(8), 8165-8174;

Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China
Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, China
Beijing Biomass Energy Technology Center, State Grid Energy Conservation Service Ltd., Beijing 100053, China
Author to whom correspondence should be addressed.
Academic Editor: Thomas E. Amidon
Received: 17 May 2015 / Revised: 17 July 2015 / Accepted: 24 July 2015 / Published: 5 August 2015
Full-Text   |   PDF [943 KB, uploaded 5 August 2015]   |  


The enzyme-assisted aqueous extraction of oil from wet microalgae was employed to avoid the energy consumption of a dewatering process. In this paper, oil-rich microalgae Scenedesmus sp. G4 was hydrolyzed by enzyme mixtures for oil extraction. The results showed that the algae concentration had the greatest influence on yield of extracted oil, and the temperature and the ratio of enzyme mixtures affected the results as follows: the maximum yield of oil extracted from Scenedesmus sp. G4 reached 86.1% under optimal conditions with an algae concentration at 2.5 g/L, temperature 30/50 °C, pH 3.5/4.5 and a cellulase:pectinase:hemicellulase ratio of 1:1:1 or 1:2:1 (w/w/w). The results also indicated that the enzyme mixtures had a significant impact on the integrity of microalgae cells and the crystallization index increased from 30.7% to around 36.0% after enzymatic hydrolysis treatment. The cell wall of Scenedesmus sp. G4 has a high content of cellulose Iα and low crystallization, which is beneficial to the oil extraction by the enzyme-assisted hydrolysis method. View Full-Text
Keywords: Scenedesmus sp.; wet microalgae; oil extraction; enzyme-assisted hydrolysis Scenedesmus sp.; wet microalgae; oil extraction; enzyme-assisted hydrolysis

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Huo, S.; Wang, Z.; Cui, F.; Zou, B.; Zhao, P.; Yuan, Z. Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4. Energies 2015, 8, 8165-8174.

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