Evaluation of Anti-Candida Potential of Piper nigrum Extract in Inhibiting Growth, Yeast-Hyphal Transition, Virulent Enzymes, and Biofilm Formation
Abstract
:1. Introduction
2. Materials and Methods
2.1. General Experimental Procedures
2.2. Plant Material and Extract Preparation
2.3. Phytochemical Analysis
2.4. Fungal Strains
2.5. In Vitro Antifungal Susceptibility Test
2.6. Time-Kill Kinetic Assay
2.7. Yeast-to-Hyphal Transition Test
2.8. Biofilm Formation Assay
2.9. Phospholipase and Proteinase Production
2.10. Hemolytic Activity Assay
2.11. Cytotoxicity Assay
2.12. In Vivo P. nigrum Toxicity
2.13. Statistical Analysis of Data
3. Results
3.1. Phytochemical Analysis
3.2. Effect of P. nigrum on C. albicans Growth
3.3. Effect of P. nigrum against C. albicans on Yeast-to-Hyphal Transition
3.4. Effect of P. nigrum on C. albicans Biofilm Formation
3.5. Effect of P. nigrum on C. albicans Hydrolytic Enzyme Secretion
3.6. In Vitro and In Vivo Assessment of the Cytotoxicity of P. nigrum Extract
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bravo-Chaucanés, C.P.; Vargas-Casanova, Y.; Chitiva-Chitiva, L.C.; Ceballos-Garzon, A.; Modesti-Costa, G.; Parra-Giraldo, C.M. Evaluation of Anti-Candida Potential of Piper nigrum Extract in Inhibiting Growth, Yeast-Hyphal Transition, Virulent Enzymes, and Biofilm Formation. J. Fungi 2022, 8, 784. https://doi.org/10.3390/jof8080784
Bravo-Chaucanés CP, Vargas-Casanova Y, Chitiva-Chitiva LC, Ceballos-Garzon A, Modesti-Costa G, Parra-Giraldo CM. Evaluation of Anti-Candida Potential of Piper nigrum Extract in Inhibiting Growth, Yeast-Hyphal Transition, Virulent Enzymes, and Biofilm Formation. Journal of Fungi. 2022; 8(8):784. https://doi.org/10.3390/jof8080784
Chicago/Turabian StyleBravo-Chaucanés, Claudia Patrícia, Yerly Vargas-Casanova, Luis Carlos Chitiva-Chitiva, Andrés Ceballos-Garzon, Geison Modesti-Costa, and Claudia Marcela Parra-Giraldo. 2022. "Evaluation of Anti-Candida Potential of Piper nigrum Extract in Inhibiting Growth, Yeast-Hyphal Transition, Virulent Enzymes, and Biofilm Formation" Journal of Fungi 8, no. 8: 784. https://doi.org/10.3390/jof8080784