Next Article in Journal
The Role of PET in Supratentorial and Infratentorial Pediatric Brain Tumors
Previous Article in Journal
Digital Innovation in Oncological Primary Treatment for Well-Being of Patients: Psychological Caring as Prompt for Enhancing Quality of Life
Article

Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity

1
Faculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, Romania
2
Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, Romania
3
Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, Romania
*
Authors to whom correspondence should be addressed.
Authors with equal contribution.
Curr. Oncol. 2021, 28(4), 2466-2480; https://doi.org/10.3390/curroncol28040225
Received: 10 May 2021 / Revised: 18 June 2021 / Accepted: 29 June 2021 / Published: 4 July 2021
Antibiotics are considered the cornerstone of modern medicine; however, currently, antibiotic resistance has become a global health issue. Antibiotics also find new uses in the treatment of other pathologies as well as cancer. The present study aimed to verify the impact of tetracycline and ampicillin in a colorectal adenocarcinoma cell line, HT-29. The effects of the two antibiotics on cell viability and nucleus were evaluated by the means of MTT assay and the Hoechst staining method, respectively. The irritant potential at vascular level of the chorioallantoic membrane was tested by the HET-CAM assay. Treatment of HT-29 cells with the two antibiotics determined different effects: (i) tetracycline induced a dose- and time-dependent cytotoxic effect characterized by decreased cell viability, changes in cells morphology, apoptotic features (nuclear fragmentation), and inhibition of cellular migration, whereas (ii) ampicillin exerted a biphasic response—cytotoxic at low doses and proliferative at high concentrations. In terms of effect on blood vessels, both antibiotics exerted a mild irritant effect. These results are promising and could be considered as starting point for further in vitro studies to define the molecular mechanisms involved in the cytotoxic/proliferative effects. View Full-Text
Keywords: antibiotics; tetracycline; ampicillin; colorectal adenocarcinoma; HET-CAM assay; Hoechst staining antibiotics; tetracycline; ampicillin; colorectal adenocarcinoma; HET-CAM assay; Hoechst staining
Show Figures

Figure 1

MDPI and ACS Style

Hut, E.-F.; Radulescu, M.; Pilut, N.; Macasoi, I.; Berceanu, D.; Coricovac, D.; Pinzaru, I.; Cretu, O.; Dehelean, C. Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity. Curr. Oncol. 2021, 28, 2466-2480. https://doi.org/10.3390/curroncol28040225

AMA Style

Hut E-F, Radulescu M, Pilut N, Macasoi I, Berceanu D, Coricovac D, Pinzaru I, Cretu O, Dehelean C. Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity. Current Oncology. 2021; 28(4):2466-2480. https://doi.org/10.3390/curroncol28040225

Chicago/Turabian Style

Hut, Emil-Florin, Matilda Radulescu, Nicolae Pilut, Ioana Macasoi, Delia Berceanu, Dorina Coricovac, Iulia Pinzaru, Octavian Cretu, and Cristina Dehelean. 2021. "Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity" Current Oncology 28, no. 4: 2466-2480. https://doi.org/10.3390/curroncol28040225

Find Other Styles

Article Access Map by Country/Region

1
Back to TopTop