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Article

Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

by
Onyinye Nwagbara
1,*,
Selina F. Darling-Reed
2,
Alicia Tucker
2,
Cynthia Harris
3,
Michael Abazinge
1,
Ronald D. Thomas
2 and
Richard D. Gragg
1
1
Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA
2
College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA
3
Institute of Public Health, Florida A&M University, Tallahassee, FL 32307, USA
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2007, 4(1), 10-14; https://doi.org/10.3390/ijerph2007010002
Submission received: 31 July 2006 / Accepted: 21 February 2007 / Published: 31 March 2007
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Abstract

Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a major environmental pollutant. In this study, the effects of this carcinogen/mutagen and one of its metabolites, benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), on human prostate carcinoma cell line DU145, were examined. Cell viability, DNA damage, and cell cycle progression were evaluated as toxic end-points. We have shown that B[a]P and BPDE inhibited cell viability following 48 hr of exposure. Furthermore, comet assay analyses revealed that both B[a]P and BPDE induced DNA strand breaks in a concentration -dependent fashion. Flow cytometric analyses showed that about 70 % of DU145 cells were arrested by B[a]P at the G1 phase, while about 76% were arrested at G1 phase by BPDE. These data reveal that B[a]P and BPDE are cytotoxic and genotoxic to DU145 prostate cancer cells.
Keywords: Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle

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MDPI and ACS Style

Nwagbara, O.; Darling-Reed, S.F.; Tucker, A.; Harris, C.; Abazinge, M.; Thomas, R.D.; Gragg, R.D. Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. Int. J. Environ. Res. Public Health 2007, 4, 10-14. https://doi.org/10.3390/ijerph2007010002

AMA Style

Nwagbara O, Darling-Reed SF, Tucker A, Harris C, Abazinge M, Thomas RD, Gragg RD. Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. International Journal of Environmental Research and Public Health. 2007; 4(1):10-14. https://doi.org/10.3390/ijerph2007010002

Chicago/Turabian Style

Nwagbara, Onyinye, Selina F. Darling-Reed, Alicia Tucker, Cynthia Harris, Michael Abazinge, Ronald D. Thomas, and Richard D. Gragg. 2007. "Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide" International Journal of Environmental Research and Public Health 4, no. 1: 10-14. https://doi.org/10.3390/ijerph2007010002

APA Style

Nwagbara, O., Darling-Reed, S. F., Tucker, A., Harris, C., Abazinge, M., Thomas, R. D., & Gragg, R. D. (2007). Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. International Journal of Environmental Research and Public Health, 4(1), 10-14. https://doi.org/10.3390/ijerph2007010002

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