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Article

Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage

by
Hongtao Yu
1,*,
Qingsu Xia
2,
Jian Yan
1,
Diogenes Herreno-Saenz
3,
Yuh-Shen Wu
4,
I-Wah Tang
2 and
Peter P. Fu
2,*
1
Department of Chemistry, Jackson State University, Jackson, MS 39217, USA
2
National Center for Toxicological Research, Jefferson, AR 72079, USA
3
Department of Pharmacology and Toxicology, School of Medicine, University of Puerto Rico, San Juan, Puerto Rico 00935
4
Hung Kuang University, Sha-Lu, Taichung, Taiwan, ROC
*
Authors to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2006, 3(4), 348-354; https://doi.org/10.3390/ijerph2006030045
Submission received: 12 July 2005 / Revised: 20 January 1995 / Accepted: 1 August 2006 / Published: 31 December 2006
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Abstract

:
Polycyclic aromatic hydrocarbons (PAHs) are a class of genotoxic environmental contaminants. We have long been interested in determining the mechanisms by which PAHs induce genotoxicity. Although the metabolic activation of PAHs leading to biological activities has been well studied, the photo-induced activation pathway has seldom reported. In this paper, we review the study of photoirradiation of PAHs with UVA irradiation results in (i) cytotoxicity and DNA damage (ii) DNA single strand cleavage; (iii) formation of 8-hydroxy-2′-deoxyguanosine adduct (8-OHdG), and (iv) formation of lipid peroxidation. Evidence has been shown that these photobiological activities are mediated by reactive oxygen species (ROS).

Introduction

Polycyclic aromatic hydrocarbons (PAHs) are a class of genotoxic environmental contaminants [1–6]. PAHs are formed from incomplete combustion of fossil fuels during both natural events and human activities [3, 5, 7, 8]. Being ubiquitous in the environment, PAHs are thought to induce tumors, primarily in the lungs, bladder and in the skin [1, 3–5, 7]. The International Agency for Research on Cancer, the United States Environmental Protection Agency, and the National Toxicology Program have classified some PAHs as probable human carcinogens [9–11]. Study of the mechanisms by which PAHs induce cancers has been the most intensive in the field of chemical carcinogenesis. The tumorigenicity and the metabolic activation pathways of PAHs have been well-studied and many books and review articles have reported on the study of carcinogenesis by PAHs [1, 3, 6, 9, 10].
It is established that PAHs require metabolic activation in order to exert biological activities, including carcinogenicity [3]. During the last several decades, study of the mechanisms by which PAHs induce tumors in experimental animals has been one of the most extensive in the field of chemical carcinogenesis [3, 6, 12]. To date, three metabolic activation pathways in vivo have been determined, namely; metabolism into bay-region diol epoxides, radical-cation intermediates, and quinones [3, 13–16] (Figure 1). Among the three activation pathways, formation of diol epoxide-derived DNA adducts is the principal metabolic activation pathway leading to cancer initiation (Figure 1). All these pathways result in binding of the ultimate metabolites with cellular DNA to form exogenous DNA adducts leading to cancer formation. Penning et al. [16] have also found that the activation pathway that forms quinone also involves the generation of reactive oxygen species (ROS) and leads to the formation of endogenous DNA adducts.
Another pathway that activates PAHs to induce various toxicities is light activation. PAHs generally absorb UV light and some also absorb visible light. Upon light absorption, PAHs are excited to their upper energy states that can initiate a series of excited state reactions leading to ROS and other reactive intermediates that can damage DNA, protein, and cell membrane, leading to acute toxicity and genotoxicity.
Skin is the largest body organ in humans and a principal target organ on cancer induced by PAHs. Phototoxicity of PAHs has been recognized more than seventy years ago [17–22]. It has also been reported that upon light irradiation, PAHs exhibit phototoxicity [17]. Thus, photoirradiation of PAHs can be an activation pathway and may play an important role on skin cancer [23–26]. However, this pathway has received much less attention. In the present review, we report that photoirradiation of PAHs can result in cytotoxicity, mutagenicity, DNA damage, and induction of lipid peroxidation.

PAH Photoirradiaion Leading to Toxicity and Genotoxicity

The photochemical and phototoxic activities of PAHs were reviewed in 2002 [17]. The review of the effects of near UV radiation on the toxic effects of PAHs in animals and plants was reported in 1996 [27]. Generally, the resulting reactive species generated due to photo-excitation can damage a variety of cellular tissues, with DNA as the principal target leading to genotoxicity [17, 27]. The possible DNA damages include: (i) Single strand cleavage, (ii) Double strand cleavage, (iii) Deletion of a base (depurination/depyrimidation), (iv) Oxidation of guanine to 8-hydroxy- or 8-oxo-guanine, (v) Thymine-thymine dimer formation, (vi) DNA covalent adducts, (vii) DNA-DNA cross-links, and (viii) DNA-protein cross-links. To date, several types of DNA damages caused by photoirradiation of PAHs have been reported, including the formation of PAH-DNA covalent adducts, DNA single strand cleavage, and formation of oxidative product 8-hydroxyguanine. When photoirradiation is performed in the presence of lipid molecules, induction of lipid peroxidation has also been determined.

Theoretical and Mechanistic Consideration

Formation of ROS

The mechanisms leading to phototoxicity by PAHs have been studied [28–42]. PAHs that contain three or more aromatic rings can absorb UVA (320 – 400 nm) and visible (> 400 nm) light. The resulting excited PAHs can act as sensitizers to transfer energy or one electron to molecular oxygen to form reactive oxygen species (ROS) [17]. It can also react with other molecules to generate reactive intermediates. Both ROS and the reactive intermediates can damage cellular constituents such as cell membrane, nucleic acids, or proteins, resulting in acute toxicity and genotoxicity including carcinogenicity, mutgenicity, and teratology.

Reactive PAH Intermediates

It has been found that upon light irradiation, anthracene, benz[a]anthracene (BA), pyrene, 5-methylbenz[a]anthracene, 7,12-dimethyl-BA (DMBA), and benzo[a]pyrene react with oxygen to form endoperoxides as intermediates and the corresponding quinones as the end products [17,43–50]. These results indicate that quinones are the stable end photoproducts of PAH light irradiation. It is proposed that the oxy-radical intermediate is formed and is responsible for DNA damage caused by concomitant exposure to benzo[a]pyrene and UV light [51]. Consequently, it is highly possible that the radical intermediates leading to the quinone formation are the species capable of causing DNA damage as well as inducing lipid peroxidation.
Multiple photoproducts can also be formed in some cases. For example, photoirradiation of DMBA with UVA light forms a series of photoproducts, including 7,12-endoperoxide, 7-hydroxymethyl-12-MBA, DMBA-7,12-quinone, 12-hydroxymethyl-7-MBA, 7-formyl-12-MBA, and 12-formyl-7-MBA [50].

Photocytotoxicity

PAHs are generally more toxic when exposed to light than without light irradiation. The phototoxicity can be more than 100-fold higher than their toxicity in the absence of light irradiation [52,53]. Yan et al. [54] found that benz[a]anthracene, benzo[a]pyrene, anthracene, benzo[ghi]perylene, indeno[1,2,3-cd]pyrene, and pyrene are highly phototoxic in Salmonella typhimurium TA102 concomitantly irradiated with UVA light.

Photomutagenicity

Azulene is a non-alternate hydrocarbon, consisting of an unsaturated seven member ring fused with an unsaturated five member ring (Figure 2). It absorbs red light in the visible region (600 nm band) for the first excited state transition and UVA light (330 nm band) for the second excited state transition and produces a beautiful blue color in aqueous solutions [55]. This is drastically different from naphthalene, which absorbs light only in the UVB region (<315 nm).
Azulene and its alkyl derivative guaiazulene, 1,4-dimethyl-7-isopropylazulene and its structural isomer naphthalene (Figure 2) are the simplest PAHs. Azulene and its derivative are popular ingredients in beauty, cosmetic, skin, and body care products [56]. The photomutagenicity of azulene and guaiazulene in Salmonella typhimurium TA98, TA100 and TA102 was determined by irradiation with UVA and visible light. Both azulene and gauiazulene exhibited photomutagenicity on TA102. Azulene was photomutagenic when irradiated by UVA light, visible light, or a combination of UVA and visible light [56]. At the same time, naphthalene is not photomutagenic or acutely phototoxic. The difference in toxicity is due primarily to the light absorption. While azulene and guaiazulene absorbs UVA and visible light, naphthalene does not.
The photomutagenicity of sixteen PAHs, all on the US EPA Priority Pollutant List, was studied [56]. Concomitant exposing the Salmonella typhimurium bacteria strain TA102 to one of the PAHs and light (1.15 J/cm2 UVA + 2.1 J/cm2 visible) without the metabolic enzyme mix S9, strong mutagenic response is observed for anthracene, benz[a]anthracene, benzo[ghi]perylene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene, and pyrene. Under the same conditions, acenaphthene, acenaphthylene, benzo[k]fluoranthene, chrysene, and fluorene are weakly photomutagenic. Benzo[b]fluoranthene, fluoranthene, naphthalene, phenanthrene, and dibenz[a,h]anthracene are not photomutagenic. These results indicate that PAHs can be activated by light and become mutagenic in Salmonella TA102 bacteria. It has been found that DMBA is phototoxic in bacteria as well as in animal or human cells and photomutagenic in Salmonella typhimurium strain TA102 [49].

Formation of DNA Adducts

In 1964, Ts’O and Lu first reported that irradiation of benzo[a]pyrene by light (>300 nm or > 340 nm) induced the formation of covalent DNA adducts, and proteins and cell membranes can be important photo-damage targets for PAH-DNA adducts [57]. Since then, it was found that irradiation on the mixture of dibenz[a,c]anthracene or dibenz[a,h]anthracene with DNA in aqueous solutions also formed covalent DNA adducts [17]. Photoirradiation of anthracene in human skin, human serum albumin, or monkey kidney epithelial cells all generated DNA adducts [39, 58].
Photoirradiation of DMBA in the presence of calf thymus DNA followed by 32P-postlabeling/TLC indicated that multiple DNA adducts were formed [49]. Comparison of the DNA adduct profiles indicates that the DNA adducts formed from photo-irradiation are different from the DNA adducts formed due to the reaction of DMBA metabolites with DNA. These results suggest that photoirradiation of DMBA can lead to genotoxicity through activation pathways different from those by microsomal metabolism of DMBA [49].

Light-Induced DNA Single Strand Cleavage by PAHs

It was reported that irradiation of benzo[a]pyrene in the presence of PM2 DNA caused the cleavage of the supercoiled form to the relaxed circular form [51]. DMBA exposed to near UV light also caused DNA single strand cleavage [59]. Kagan et al determined that anthracene [40] can cause pBR322 DNA single strand cleavage.
A systematic light-induced DNA single strand cleavage study has been carried out for various PAHs using UVA light and ΦX174 plasmid DNA [47, 60–64]. The extent of DNA single strand cleavage was dependent on both light and PAH doses. Therefore, by fixing the light dose at 170 J/cm2 (1 h irradiation), a relative DNA photocleavage efficiency indicator, C25, the concentration at which 25% of the original supercoiled DNA is converted into relaxed, open circular DNA upon the combination of a PAH and light irradiation, is determined. The C25 can be used to compare relative DNA photocleavage efficiency for various PAHs. The smaller the C25 values, the more efficient is a PAH toward DNA single strand cleavage.
The C25 values depend on both (i) the structure of the PAHs and (ii) the ring size and arrangement of the rings. A larger ring does not necessarily mean higher DNA photocleavage efficiency. The three-ring anthrancene is a stronger DNA photocleaver than the four-ring pyrene, but is similar to the four-ring chrysene and BA and the five ring benzo[a]pyrene [62]. Metabolic products of benzo[a]pyrene are all more efficient in causing DNA photocleavage [64]. As shown in Table 1, methyl substitution on BA has an interesting effect on their DNA photocleavage efficiency. Methyl substitution at 4, 5, 6, 8, 9, 10 positions does not affect the photocleavage efficiency for BA, but methyl substitution at other positions, especially at 7 and 12 positions, decreases the photocleavage efficiency for BA. This structure-photocleavage efficiency relationship match the gap between the highest occupied molecular orbital and lowest unoccupied molecular orbital (HOMO-LUMO) for these methyl substituted BAs [47].
Mechanistic studies reveal that singlet oxygen, superoxide and other radicals intermediates, including possible PAH radicals, are involved in causing DNA single strand photocleavage by PAHs [47, 60, 62, 64]. Both an oxygen-dependent and an independent pathway cause the DNA photocleavage. From PAH to PAH, the involvement of one or the other reactive species in DNA photocleavage can be different. In summary, the efficiency of light-induced DNA cleavage by PAHs is closely related to the photochemical reaction of the PAHs and the presence of chemicals or solvents that affect the photochemical reaction or the production of reactive intermediates.

Formation of 8-Hydroxyguanine (8-OHdG) Adduct

It was reported that benzo[a]pyrene exposed to fluorescent light in mammalian cells induced 8-OHdG adduct [37]. Liu et al. reported that benzo[a]pyrene enhances the formation of 8-OHdG formation by UVA radiation in calf thymus DNA and cultured cells [65]. The level of 8-OHdG adduct formed in cultured cells was much higher than that in calf thymus DNA. These results suggest that the metabolites of benzo[a]pyrene exhibit higher capability in induction of 8-OH-dG [65]. The subsequent study revealed that its metabolite, benzo[a]pyrene bay-region diolepoxide combined with UVA synergistically induced 8-OHdG in a level about 25-fold higher than benzo[a]pyrene. The mechanistic study indicated that the formation of 8-OHdG was mediated by reactive oxygen species [66].

Other DNA Damage

The phototoxicity and photomutagenicity of azulene was studied in human skin Jurkat T-cells by Comet assay. Azulene exposed to UVA light caused strand cleavage on DNA in the T-cell nucleus or pure Φ174 plasmid DNA in solution [67].

Formation of Lipid Peroxidation

The authors are also interested in determining whether or not photoirradiation of PAHs can lead to induce lipid peroxidation. The approach is to perform the photoirradiation of a series of chrysene and its isomeric methylchrysenes and ethylchrysenes by UVA light in the presence of a lipid, methyl linoleate. Upon peroxidation, methyl linoleate is converted into the isomeric methyl linoleate 9- and 13-hydroperoxides, both in trans- and cis-forms (Figure 3). We have previously reported the employment of methyl linoleate for study of lipid peroxidation induced by chrysene, 4-methylchrysene, 5-methylchrysene, 1-ethylchrysene, 2-ethylchrysene, 3-ethylchrysene, 4-ethylchrysene, and 5-ethylchrysene, concomitantly exposed to UVA light (unpublished data). The results indicate that all are capable of inducing lipid peroxidation (Figure 4). The results also indicate while 4-and 5-ethylchryses induce lipid peroxidation at a level similar to that of chrysene, 1-, 2-, and 3-ethylchrysene are weaker than chrysene in induction of lipid peroxidation.

Conclusions and Perspectives

Skin is a principal target organ on cancer induced by PAHs. For the Goeckerman therapy of psoriasis, coal tar is topically applied on the skin followed by UV light irradiation. This treatment has an increased risk of developing cutaneous cancers [68]. Roofers and highway asphalt workers also have a high risk to be exposed to both PAHs and light [69]. In the modern age we humans are in, the automobile exhaust emits PAHs. These PAHs in the air can be absorbed by skin. Since human skin is exposed to light, it is of particular importance and significance to investigate human health risks posed by exposure to the combination of PAHs and light.
Ijerph 03 00348f1 1024
Figure 1. The three principal metabolic activation pathways of benzo[a]pyrene leading to tumour initiation.
Figure 1. The three principal metabolic activation pathways of benzo[a]pyrene leading to tumour initiation.
Ijerph 03 00348f1
Ijerph 03 00348f2 1024
Figure 2. Chemical structures of azulene, guaiazulene and naphthalene
Figure 2. Chemical structures of azulene, guaiazulene and naphthalene
Ijerph 03 00348f2
Ijerph 03 00348f3 1024
Figure 3. Peroxidation of methyl linoleate into the isomeric 9- and 13-hydroperoxides.
Figure 3. Peroxidation of methyl linoleate into the isomeric 9- and 13-hydroperoxides.
Ijerph 03 00348f3
Ijerph 03 00348f4 1024
Figure 4. Induction of lipid peroxidation by photoirration of chrysene and its methylated and ethylated derivatives by UVA light.
Figure 4. Induction of lipid peroxidation by photoirration of chrysene and its methylated and ethylated derivatives by UVA light.
Ijerph 03 00348f4
Table
Table 1. Relative DNA photocleavage efficiency expressed by C25[47]
Table 1. Relative DNA photocleavage efficiency expressed by C25[47]
Benz[a]anthracenes (BA)C25(μM)Benzo[a]pyrene (BaP)derivativesC25(μM)
BA18BaP6.0
1-Methyl-BA601-Hydroxy-B[a]P0.6
2-Methyl-BA743-Hydroxy-B[a]P2.5
3-Methyl-BA346-Acetoxy-B[a]P1.3
4-Methyl-BA127-Hydroxy-B[a]P0.1
5-Methyl-BA139-Hydroxy-B[a]P1.3
6-Methyl-BA20B[a]P-3,6-quinone3.9
7-Methyl-BA~100B[a]P-1,6-quinone2.5
7-Hydroxymethyl-BA~100B[a]P-7,8-dihydrodiol1.1
8-Methyl-BA18
9-Methyl-BA17PAHs
10-Methyl-BA12Naphtahlenend
11-Methyl-BA42Anthracene8.3
12-Methyl-BA93Chrysene5.2
12-Hydroxymethyl-BA~100Pyrene51
7,12-Dithemyl-BA~100BA18
BA-7,12-dione11.8BaP6.0

Acknowledgement

This research was supported by the National Institutes of Health: NIH SCORE S06 GM08047, NIH-RCMI G12RR13459 and the US Army Research Office DAAD 1901-1-0733 to JSU.

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MDPI and ACS Style

Yu, H.; Xia, Q.; Yan, J.; Herreno-Saenz, D.; Wu, Y.-S.; Tang, I.-W.; Fu, P.P. Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage. Int. J. Environ. Res. Public Health 2006, 3, 348-354. https://doi.org/10.3390/ijerph2006030045

AMA Style

Yu H, Xia Q, Yan J, Herreno-Saenz D, Wu Y-S, Tang I-W, Fu PP. Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage. International Journal of Environmental Research and Public Health. 2006; 3(4):348-354. https://doi.org/10.3390/ijerph2006030045

Chicago/Turabian Style

Yu, Hongtao, Qingsu Xia, Jian Yan, Diogenes Herreno-Saenz, Yuh-Shen Wu, I-Wah Tang, and Peter P. Fu. 2006. "Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage" International Journal of Environmental Research and Public Health 3, no. 4: 348-354. https://doi.org/10.3390/ijerph2006030045

APA Style

Yu, H., Xia, Q., Yan, J., Herreno-Saenz, D., Wu, Y. -S., Tang, I. -W., & Fu, P. P. (2006). Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage. International Journal of Environmental Research and Public Health, 3(4), 348-354. https://doi.org/10.3390/ijerph2006030045

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