Molecular Transmission Network of Newly Reported HIV Infections in Pengzhou, Sichuan Province: A Study Based on Genomics and Spatial Epidemiology
Abstract
:1. Introduction
2. Materials and Methods
2.1. Research Object
2.2. Sample Collection and Preservation
2.3. HIV-1 Genotype Drug Resistance Detection
2.4. Sequence Arrangement and Subtype Classification
2.5. The Construction of a Molecular Network Transmission Model
2.6. Statistical Analysis
2.7. Moran’s I Index of Local Spatial Autocorrelation
3. Results
3.1. Basic Situation
3.2. Influencing Factors of HIV-1 Molecular Transmission Network
3.3. Characteristic Analysis of HIV Molecular Transmission Network
3.4. Spatial Analysis of HIV Molecular Transmission Network
4. Discussion
Limitations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- UNAIDS. GLOBAL ISSUES AIDS. Available online: https://www.un.org/en/global-issues/aids (accessed on 21 August 2022).
- UNAIDS. UNAIDS Global AIDS Update 2022—In Danger. Available online: https://indanger.unaids.org/ (accessed on 21 August 2022).
- Xiumin, G.; Decai, Z.; Yan, Z.; Ye, M.; Yasong, W.; Wisdom, B. Progress on national antiretroviral treatment program during 2003-2021 in China. Chin. J. Aids STD 2022, 28, 642–646. [Google Scholar] [CrossRef]
- UNAIDS. 2021 UNAIDS Global AIDS Update—Confronting Inequalities—Lessons for Pandemic Responses from 40 Years of AIDS. 2021. Available online: https://www.unaids.org/en/resources/documents/2021/2021-global-aids-update (accessed on 21 August 2022).
- Shi, Y.Y.; Lian, J.; Duan, Z.H.; Liu, F.; He, Q.Y.; Fan, S.F.; Zhu, Y.F. Epidemic characteristics of HIV/AIDS in Chengdu during 2015–2018. Chin. J. Aids STD 2020, 26, 1076–1079. [Google Scholar] [CrossRef]
- Ragonnet-Cronin, M.; Hu, Y.W.; Morris, S.R.; Sheng, Z.; Poortinga, K.; Wertheim, J.O. HIV transmission networks among transgender women in Los Angeles County, CA, USA: A phylogenetic analysis of surveillance data. Lancet HIV 2019, 6, e164–e172. [Google Scholar] [CrossRef] [PubMed]
- Yan, H.; He, W.; Huang, L.; Wu, H.; Liang, Y.; Li, Q.; Shui, J.; Wang, C.; Dzakah, E.E.; Han, Z.; et al. The Central Role of Nondisclosed Men Who Have Sex With Men in Human Immunodeficiency Virus-1 Transmission Networks in Guangzhou, China. Open Forum Infect. Dis. 2020, 7, ofaa154. [Google Scholar] [CrossRef] [PubMed]
- Han, X.; Zhao, B.; An, M.; Zhong, P.; Shang, H. Molecular network-based intervention brings us closer to ending the HIV pandemic. Front. Med. 2020, 14, 136–148. [Google Scholar] [CrossRef] [Green Version]
- Zhang, J.; Xu, K.; Jiang, J.; Fan, Q.; Ding, X.; Zhong, P.; Xing, H.; Chai, C.; Pan, X. Combining molecular network analysis and field epidemiology to quantify local HIV transmission and highlight ongoing epidemics. Int. J. Infect. Dis. 2022, 128, 187–193. [Google Scholar] [CrossRef]
- Jiang, H.; Tang, K.L.; Huang, J.H.; Li, J.J.; Liang, S.S.; Liu, X.H.; Pang, X.W.; Zhu, Q.Y.; Chen, H.H.; Zhou, Y.J.; et al. Analysis of HIV transmission hotspots and characteristics of cross-regional transmission in Guangxi Zhuang Autonomous Region based on molecular network. Zhonghua Liu Xing Bing Xue Za Zhi 2022, 43, 1423–1429. [Google Scholar] [CrossRef]
- Lebedev, A.; Kuznetsova, A.; Kim, K.; Ozhmegova, E.; Antonova, A.; Kazennova, E.; Tumanov, A.; Mamatkulov, A.; Kazakova, E.; Ibadullaeva, N.; et al. Identifying HIV-1 Transmission Clusters in Uzbekistan through Analysis of Molecular Surveillance Data. Viruses 2022, 14, 1675. [Google Scholar] [CrossRef]
- Nduva, G.M.; Otieno, F.; Kimani, J.; Wahome, E.; McKinnon, L.R.; Cholette, F.; Majiwa, M.; Masika, M.; Mutua, G.; Anzala, O.; et al. Quantifying rates of HIV-1 flow between risk groups and geographic locations in Kenya: A country-wide phylogenetic study. Virus Evol. 2022, 8, veac016. [Google Scholar] [CrossRef]
- Statistics SPBo. 2021 Sichuan Provincial Health Statistics Yearbook. 2022. Available online: https://dict.cnki.net/index (accessed on 27 August 2022).
- Chinese Center for Disease Control and Prevention. Guideline for HIV-1 Genotyping Drug Resistance Testing and Quality Assurance; Chinese Center for Disease Control and Prevention: Beijing, China, 2013.
- Chinese guidelines for diagnosis and treatment of HIV/AIDS (2021 edition). Zhonghua Nei Ke Za Zhi 2021, 60, 1106–1128. [CrossRef]
- Moran, P.A. Notes on continuous stochastic phenomena. Biometrika 1950, 37, 17–23. [Google Scholar] [CrossRef] [PubMed]
- Rheingans, R.; Cumming, O.; Anderson, J.; Showalter, J. Estimating Inequities in Sanitation-Related Disease Burden and Estimating the Potential Impacts of Pro-Poor Targeting; London School of Hygiene and Tropical Medicine: London, UK, 2012. [Google Scholar]
- Ling, S.U.; Li, Y.E.; Liu, Y.; Zou, J.; Wang, S.; Luo, S.; Hou, X.; Yuan, D.; Yang, H.; Wei, D. HIV-1 molecular epidemiological survey of people over 50 years old in Sichuan. Chin. J. AIDS STD 2018, 24, 788–792. [Google Scholar]
- Ling, S.; Hong, Y.; Ying, H.; Li, Y.; Dan, Y.; Wei, D.; Zhou, G.; Jia, Y.; Liu, H.; Qin, G. Molecular epidemiological study on HIV-1 in Sichuan province in 2014. Chin. J. AIDS STD 2016, 22, 946–949. [Google Scholar]
- Zhong, P. Progress in research and practice of molecular epidemiology of HIV-1. Electron. J. Emerg. Infect. Dis. 2019, 4, 137–144. [Google Scholar]
- Su, L.; Liang, S.; Hou, X.; Zhong, P.; Wei, D.; Fu, Y.; Ye, L.; Xiong, L.; Zeng, Y.; Hu, Y.; et al. Impact of worker emigration on HIV epidemics in labour export areas: A molecular epidemiology investigation in Guangyuan, China. Sci. Rep. 2018, 8, 16046. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Shiyun, L.; Jie, D.; Pengyan, S.; Youfang, L.; Lijuan, D.; Huichao, C.; Xiaomei, J.; Min, Y.; Yanling, M. Analysis on characteristics of HIV-1 molecular networks in men who have sex with men in Kunming, 2016–2018. Chin. J. Epidemiol. 2020, 41, 1121–1125. [Google Scholar]
- Huijie, Z.; Liqian, X.; Hao, W.; Qingmei, L.; Zhigang, H. Characteristics of HIV-1 molecular transmission network among HIV-1 positive men who have sex with men in Guangzhou from 2008 to 2015. Chin. J. Aids STD 2020, 26, 1169–1173. [Google Scholar]
- Liu, Z.; Tang, X.; Liu, Y.; Zhang, L.; Yang, Y.; Zheng, Y.; Liu, W.; Jike, C.; Mai, H.; Fan, H.; et al. HIV Prevention and Health Poverty Alleviation—Liangshan Prefecture, Sichuan Province, China, 2017–2020. China CDC Weekly 2021, 3, 1031–1035. [Google Scholar] [CrossRef]
- Li, N.; Luo, G.S.; Liao, R.P.; Chen, L.; Li, L.J.; Xiao, W. Characteristic analysis of newly reported HIV infections among the eldly population in Pengzhou, Sichuan, 2014–2018. China Trop. Med. 2020, 20, 53–55. [Google Scholar]
- WHO. World AIDS Day|Older Age Groups: China’s HIV-Infected Population Reports Highest Multi-Year Growth Rate. 2021. Available online: https://mp.weixin.qq.com/s/ikhndpW3a4k39sJ_n_4hIw (accessed on 27 August 2022).
- Ling, S.U.; Xiao, L.; Yang, H. Analysis onRecent Infected HIV-1Patients among Newly Reported HIV Cases in Sichuan from 2010–014. Chin. J. AIDS STD 2015, 25, 942–945. [Google Scholar] [CrossRef]
- GAO, Y.; LU, H.Z. Review of influencing factors of high-risk sexual behaviors among elderly people. Chin. J. Aids STD 2020, 26, 354,452–454. [Google Scholar] [CrossRef]
- Jiang, G.Y.; Guo, W.; Pei, Y.X.; Cai, C.; Wu, G.H.; Zhou, C.; Lu, R.R.; Chen, Z.L. Survey on extramarital sexual behaviors and HIV infection in middle-aged and elderly people aged 50 and above in selected areas of Chongqing. Chin. J. Epidemiol. 2018, 39, 1438–1442. [Google Scholar]
- Ge, Z.; Feng, Y.; Li, K.; Lv, B.; Zaongo, S.D.; Sun, J.; Liang, Y.; Liu, D.; Xing, H.; Wei, M.; et al. CRF01_AE and CRF01_AE Cluster 4 Are Associated with Poor Immune Recovery in Chinese Patients Under Combination Antiretroviral Therapy. Clin. Infect. Dis. 2021, 72, 1799–1809. [Google Scholar] [CrossRef] [PubMed]
- Jiang, H.; Lan, G.; Zhu, Q.; Feng, Y.; Liang, S.; Li, J.; Zhou, X.; Lin, M.; Shao, Y. Impacts of HIV-1 Subtype Diversity on Long-Term Clinical Outcomes in Antiretroviral Therapy in Guangxi, China. J. Acquir. Immune Defic. Syndr. 2022, 89, 583–591. [Google Scholar] [CrossRef]
- Li, X.; Li, W.; Zhong, P.; Fang, K.; Zhu, K.; Musa, T.H.; Song, Y.; Du, G.; Gao, R.; Guo, Y.; et al. Nationwide Trends in Molecular Epidemiology of HIV-1 in China. AIDS Res. Hum. Retrovir. 2016, 32, 851–859. [Google Scholar] [CrossRef]
- Feng, C.B.; Liu, Y. Causes of sexual infection of 473 newly reported HIV/AIDS cases among men aged 50 years and over in Luzhou. J. Prev. Med. Inf. 2022, 38, 13–16. [Google Scholar]
- Oster, A.M.; Wertheim, J.O.; Hernandez, A.L.; Ocfemia, M.C.; Saduvala, N.; Hall, H.I. Using Molecular HIV Surveillance Data to Understand Transmission between Subpopulations in the United States. J. Acquir. Immune Defic. Syndr. 2015, 70, 444–451. [Google Scholar] [CrossRef]
- Statistics Bureau of Pengzhou. Statistical Bulletin of Pengzhou National Economic and Social Development in 2019; Statistics Bureau of Pengzhou: Chengdu, China, 2020.
Classification | Node ≥ 2 | Total | n% | OR (95%CI) a | AOR (95%CI) b | |
---|---|---|---|---|---|---|
n | % | |||||
Gender | ||||||
male | 138 | 81.18 | 348 | 75.16 | 1 | 1 |
female | 32 | 18.82 | 115 | 24.84 | 0.59 (0.37~0.93) ** | 0.66 (0.39~1.12) * |
Age | ||||||
<50 | 22 | 12.94 | 121 | 26.13 | 1 | 1 |
≥50 | 148 | 87.06 | 342 | 73.87 | 3.43 (2.06~5.71) *** | 2.10 (1.13~3.92) ** |
Occupation | ||||||
farmer | 149 | 87.65 | 368 | 79.48 | 1 | 1 |
employed | 21 | 12.35 | 95 | 20.52 | 0.42 (0.25~0.71) ** | 0.46 (0.25~0.87) ** |
Education Level | ||||||
illiteracy | 24 | 14.12 | 47 | 10.15 | 1 | 1 |
primary school | 93 | 54.71 | 218 | 47.08 | 0.71 (0.38~1.34) | 0.82 (0.39~1.69) |
junior high school and above | 53 | 31.18 | 198 | 42.76 | 0.35 (0.18~0.67) ** | 0.61 (0.28~1.34) * |
Marital status | ||||||
single/divorced/widow | 66 | 38.82 | 172 | 37.15 | 1 | |
wedlock | 104 | 61.18 | 291 | 62.85 | 0.89 (0.61~1.32) | |
Route of infection | ||||||
heterosexual transmission | 166 | 97.65 | 443 | 95.68 | 1 | 1 |
homosexual transmission | 4 | 2.35 | 20 | 4.32 | 0.42 (0.14~1.27) * | 1.64 (0.45~5.95) * |
Subtype | ||||||
CRF07_BC | 21 | 12.35 | 159 | 34.34 | 1 | 1 |
CRF01_AE | 114 | 67.06 | 237 | 51.19 | 6.09 (3.60~10.30) *** | 5.25 (3.04~9.07) *** |
B | 34 | 20 | 45 | 9.72 | 20.31 (8.94~46.13) *** | 19.08 (8.03~45.32) *** |
CRF08_BC | 1 | 0.59 | 15 | 3.24 | 0.47 (0.06~3.76) | 0.39 (0.05~3.20) * |
other | 0 | 0 | 7 | 1.51 | ||
Drug resistance | ||||||
YES | 3 | 1.76 | 20 | 4.32 | 1 | 1 |
NO | 167 | 98.24 | 443 | 95.68 | 3.43 (0.99~11.88) * | 3.85 (0.92~16.03) * |
First CD4+ T cell count | ||||||
≤200 | 73 | 42.94 | 203 | 43.84 | 1 | |
>200 | 89 | 52.35 | 243 | 52.48 | 1.03 (0.70~1.52) | |
unknown | 8 | 4.71 | 17 | 3.67 |
Town | All | CRF01_AE | CRF07_BC | B | CRF08_BC | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Intra-Township | Inter-Township | Proportion of Transmission Inter-Township (%) | Intra-Township | Inter-Township | Proportion of Transmission Inter-Township (%) | Intra-Township | Inter-Township | Proportion of Transmission Inter-Township (%) | Intra-Township | Inter-Township | Proportion of Transmission Inter-Township (%) | Intra-Township | Inter-Township | Proportion of Transmission Inter-Township (%) | |
Cifengzhen | 0 | 14 | 100 | 0 | 2 | 100 | 0 | 0 | / | 0 | 0 | / | 0 | 0 | / |
Danjingshanzhen | 0 | 30 | 100 | 0 | 12 | 100 | 0 | 6 | 100 | 0 | 0 | / | 0 | 0 | / |
Xinxingzhen | 0 | 14 | 100 | 0 | 14 | 100 | 0 | 0 | / | 0 | 0 | / | 0 | 0 | / |
Jiuchizhen | 2 | 92 | 97.87 | 0 | 16 | 100 | 0 | 0 | / | 2 | 76 | 97.44 | 0 | 0 | / |
Longmenshanzhen | 1 | 38 | 97.44 | 1 | 38 | 97.44 | 0 | 0 | / | 0 | 0 | / | 0 | 0 | / |
Sanjiezhen | 2 | 54 | 96.43 | 1 | 0 | 0 | 0 | 0 | / | 1 | 53 | 98.15 | 0 | 1 | 100 |
Junlezhen | 4 | 93 | 95.88 | 1 | 5 | 83.33 | 0 | 3 | 100 | 3 | 85 | 96.59 | 0 | 0 | / |
Aopingzhen | 3 | 62 | 95.38 | 2 | 28 | 93.33 | 1 | 5 | 83.33 | 0 | 27 | 100 | 0 | 2 | 100 |
Tongjizhen | 7 | 112 | 94.12 | 5 | 45 | 90 | 1 | 7 | 87.5 | 1 | 67 | 98.53 | 0 | 0 | / |
Zhihezhen | 8 | 109 | 93.16 | 4 | 25 | 86.21 | 1 | 5 | 83.33 | 3 | 72 | 96 | 0 | 1 | 100 |
Xiaoyudongzhen | 4 | 50 | 92.59 | 3 | 50 | 94.34 | 1 | 0 | 0 | 0 | 0 | / | 0 | 0 | / |
Shengpingzhen | 10 | 105 | 91.3 | 7 | 15 | 68.18 | 0 | 0 | / | 3 | 90 | 96.77 | 0 | 0 | / |
Hongyanzhen | 2 | 20 | 90.91 | 2 | 6 | 75 | 0 | 2 | 100 | 0 | 12 | 100 | 0 | 0 | / |
Guihuazhen | 1 | 7 | 87.5 | 1 | 4 | 80 | 0 | 2 | 100 | 0 | 0 | / | 0 | 1 | 100 |
Gexianshanzhen | 7 | 40 | 85.11 | 4 | 11 | 73.33 | 3 | 5 | 62.5 | 0 | 24 | 100 | 0 | 0 | / |
Longfengzhen | 7 | 32 | 82.05 | 3 | 27 | 90 | 4 | 5 | 55.56 | 0 | 0 | / | 0 | 0 | / |
Tianpengzhen | 93 | 332 | 78.12 | 15 | 92 | 85.98 | 3 | 12 | 80 | 75 | 228 | 75.25 | 0 | 0 | / |
Mengyangzhen | 12 | 37 | 75.51 | 6 | 14 | 70 | 6 | 1 | 14.29 | 0 | 21 | 100 | 0 | 1 | 100 |
Lichunzhen | 54 | 109 | 66.87 | 54 | 108 | 66.67 | 0 | 1 | 100 | 0 | 0 | / | 0 | 0 | / |
Bailuzhen | 1 | 0 | 0 | 0 | 0 | / | 1 | 0 | 0 | 0 | 0 | / | 0 | 0 | / |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Yuan, D.; Zhong, X.; Li, Y.; He, Q.; Li, N.; Li, H.; Liu, Y.; Li, L.; Zhang, L.; Yang, Y.; et al. Molecular Transmission Network of Newly Reported HIV Infections in Pengzhou, Sichuan Province: A Study Based on Genomics and Spatial Epidemiology. Int. J. Environ. Res. Public Health 2023, 20, 2523. https://doi.org/10.3390/ijerph20032523
Yuan D, Zhong X, Li Y, He Q, Li N, Li H, Liu Y, Li L, Zhang L, Yang Y, et al. Molecular Transmission Network of Newly Reported HIV Infections in Pengzhou, Sichuan Province: A Study Based on Genomics and Spatial Epidemiology. International Journal of Environmental Research and Public Health. 2023; 20(3):2523. https://doi.org/10.3390/ijerph20032523
Chicago/Turabian StyleYuan, Dan, Xia Zhong, Yiping Li, Qinying He, Na Li, Hanqi Li, Yang Liu, Ling Li, Linglin Zhang, Yi Yang, and et al. 2023. "Molecular Transmission Network of Newly Reported HIV Infections in Pengzhou, Sichuan Province: A Study Based on Genomics and Spatial Epidemiology" International Journal of Environmental Research and Public Health 20, no. 3: 2523. https://doi.org/10.3390/ijerph20032523