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Article

Bacterial Isolates and Antibiotic Resistance of Escherichia coli Isolated from Fresh Poultry Excreta Used for Vegetable Farming in Freetown, Sierra Leone

by
Alie H. D. Mansaray
1,*,
Dennis P. Y. Yankson
1,
Raymonda A. B. Johnson
1,
Francis L. Moses
2,3,
Joseph Sam Kanu
4,5,
Ibrahim Franklyn Kamara
6,
Rony Zachariah
7,
Ajay M. V. Kumar
8,9,10 and
Kalaiselvi Selvaraj
11
1
Senior Agriculture Officer, Crops Division, Ministry of Agriculture and Forestry, West Wing, Youyi Building, Brookfields, Freetown 00232, Sierra Leone
2
Directorate of Reproductive and Child Health, Ministry of Health and Sanitation, Youyi Building, Brookfields, Freetown 00232, Sierra Leone
3
Faculty of Basic Medical Sciences, College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown 00232, Sierra Leone
4
National Disease Surveillance Program, Ministry of Health and Sanitation, Sierra Leone National Public Health Emergency Operations Centre, Cockerill, Wilkinson Road, Freetown 00232, Sierra Leone
5
Department of Community Health, Faculty of Clinical Sciences, College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown 00232, Sierra Leone
6
World Health Organization, 21A-B Riverside, Off King Harman Road Freetown, Freetown 00232, Sierra Leone
7
Special Program for Research and Training in Tropical Diseases (TDR), World Health Organization, Avenue Appia 20, 1211 Geneva 27, Switzerland
8
International Union against Tuberculosis and Lung Disease, 68 Boulevard Saint Michel, 75006 Paris, France
9
International Union against Tuberculosis and Lung Disease, South-East Asia Office, C-6 Qutub Institutional Area, New Delhi 110016, India
10
Yenepoya Medical College, Yenepoya (Deemed to be University), University Road, Deralakatte, Mangalore 575018, India
11
All India Institute of Medical Sciences, Nagpur 441108, India
 
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Int. J. Environ. Res. Public Health 2022, 19(9), 5405; https://doi.org/10.3390/ijerph19095405
Submission received: 23 February 2022 / Revised: 28 March 2022 / Accepted: 13 April 2022 / Published: 29 April 2022
(This article belongs to the Special Issue Operational Research and Capacity Building to Tackle Antimicrobial Resistance in Sierra Leone)
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Abstract

The transfer of antibiotic resistance from animals to humans is of concern in recent times. One potential source of such transfer is the untreated poultry excreta used as manure in farming. We aim to identify bacterial isolates and antibiotic susceptibility patterns of Escherichia coli in poultry excreta in Sierra Leone. This was a cross-sectional study of fresh poultry excreta samples collected from four commercial poultry sites in Freetown, Sierra Leone, from June–September 2021. Bacterial isolates were tested against eight antibiotics using established standards. Of 100 samples, 93 showed Escherichia coli (93%): of those, eight isolates also had Salmonella (8%). E. coli was 100% resistant to all three ‘Watch’ drugs (erythromycin, cefoxitin and streptomycin) and tetracycline. E. coli was least resistant to ampicillin (12%), followed by chloramphenicol (35%). The prevalence of multidrug resistance was 95.6%. Multiple antibiotic resistance index ranged from 0.5–0.7 (optimal < 0.2), indicating high prior exposure to antibiotics in these poultries. Such high levels of resistance in E. coli isolated from poultry excreta could pose a serious threat to humans. We recommend (i) routine surveillance to monitor antibiotic resistance in poultry excreta, (ii) using poultry excreta as manure only after treatment and (iii) restricting the use of antibiotics as prophylactics and growth promoters in poultry feeds.
Keywords: one health; surveillance; antimicrobial resistance; microbial sensitivity tests; operational research; bacterial isolates; Escherichia coli; poultry excreta; SORT IT; Sierra Leone one health; surveillance; antimicrobial resistance; microbial sensitivity tests; operational research; bacterial isolates; Escherichia coli; poultry excreta; SORT IT; Sierra Leone

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MDPI and ACS Style

Mansaray, A.H.D.; Yankson, D.P.Y.; Johnson, R.A.B.; Moses, F.L.; Kanu, J.S.; Kamara, I.F.; Zachariah, R.; Kumar, A.M.V.; Selvaraj, K. Bacterial Isolates and Antibiotic Resistance of Escherichia coli Isolated from Fresh Poultry Excreta Used for Vegetable Farming in Freetown, Sierra Leone. Int. J. Environ. Res. Public Health 2022, 19, 5405. https://doi.org/10.3390/ijerph19095405

AMA Style

Mansaray AHD, Yankson DPY, Johnson RAB, Moses FL, Kanu JS, Kamara IF, Zachariah R, Kumar AMV, Selvaraj K. Bacterial Isolates and Antibiotic Resistance of Escherichia coli Isolated from Fresh Poultry Excreta Used for Vegetable Farming in Freetown, Sierra Leone. International Journal of Environmental Research and Public Health. 2022; 19(9):5405. https://doi.org/10.3390/ijerph19095405

Chicago/Turabian Style

Mansaray, Alie H. D., Dennis P. Y. Yankson, Raymonda A. B. Johnson, Francis L. Moses, Joseph Sam Kanu, Ibrahim Franklyn Kamara, Rony Zachariah, Ajay M. V. Kumar, and Kalaiselvi Selvaraj. 2022. "Bacterial Isolates and Antibiotic Resistance of Escherichia coli Isolated from Fresh Poultry Excreta Used for Vegetable Farming in Freetown, Sierra Leone" International Journal of Environmental Research and Public Health 19, no. 9: 5405. https://doi.org/10.3390/ijerph19095405

APA Style

Mansaray, A. H. D., Yankson, D. P. Y., Johnson, R. A. B., Moses, F. L., Kanu, J. S., Kamara, I. F., Zachariah, R., Kumar, A. M. V., & Selvaraj, K. (2022). Bacterial Isolates and Antibiotic Resistance of Escherichia coli Isolated from Fresh Poultry Excreta Used for Vegetable Farming in Freetown, Sierra Leone. International Journal of Environmental Research and Public Health, 19(9), 5405. https://doi.org/10.3390/ijerph19095405

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