Soil Heavy Metal Content and Enzyme Activity in Uncaria rhynchophylla-Producing Areas under Different Land Use Patterns
Abstract
:1. Introduction
2. Materials and Methods
2.1. General Situation of Research Area
2.2. Sample Collection and Testing
2.3. Evaluation Methods
- (1)
- Single pollution index method:
- (2)
- Nemero pollution index method:
2.4. Data Analysis Methods
3. Results and Analysis
3.1. Soil Heaavy Metal Content and Pollution Level under Different Land Use Patterns
3.2. Characteristics of Soil Enzyme Activity under Different Land Use Patterns
3.3. Correlation between Soil Heavy Metals and Enzyme Activities under Different Land Use Patterns
4. Discussion
4.1. Characteristics of Soil Heavy Metal Content and Enzyme Activity in Uncaria rhynchophylla-Producing Areas under Different Land Use Patterns
4.2. Correlation between Soil Heavy Metal Content and Enzyme Activity under Different Land Use Patterns
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Tan, X.P.; He, J.H.; Guo, Z.M.; Wang, Z.Q.; Nie, Y.X.; Ye, Q.; He, W.X.; Shen, W.J. Research Progresses on Soil Enzymes as Indicators of Soil Health and Their Responses to Heavy Metal Pollution. Acta Pedol. Sin. 2022, 59, 1–15. (In Chinese) [Google Scholar] [CrossRef]
- Zhang, J.; Li, H.; Zhou, Y.; Dou, L.; Cai, L.; Mo, L.; You, J. Bioavailability and soil-to-crop transfer of heavy metals in farmland soils: A case study in the Pearl River Delta, South China. Environ. Pollut. 2018, 235, 710–719. [Google Scholar] [CrossRef]
- Srivastava, V.; Sarkar, A.; Singh, S.; Singh, P.; De Araujo, A.S.; Singh, R.P. Agroecological responses of heavy metal pollution with special emphasis on soil health and plant performances. Front. Environ. Sci. 2017, 5, 64. [Google Scholar] [CrossRef]
- Zhang, T.L.; Wang, X.X. Prevention and Remediation of Soil Contamination to Strengthen the Fo undation for Green and High-Quality Agricultural Development in China. Acta Pedol. Sin. 2019, 56, 251–258. (In Chinese) [Google Scholar] [CrossRef]
- Tripathy, V.; Basak, B.; Varghese, T.S.; Saha, A. Residues and contaminants in medicinal herbs—A review. Phytochem. Lett. 2015, 14, 67–78. [Google Scholar] [CrossRef]
- Wang, C.; Liu, C.; Hu, Q.; Wang, H.; Gao, J.Y. Pollution status of heavy metals in herbal medicine and their traceability & control in the whole chain production process. Chin. J. Environ. Eng. 2021, 15, 3789–3800. (In Chinese) [Google Scholar] [CrossRef]
- Geng, C.-A.; Yang, T.-H.; Huang, X.-Y.; Ma, Y.-B.; Zhang, X.-M.; Chen, J.-J. Antidepressant potential of Uncaria rhynchophylla and its active flavanol, catechin, targeting melatonin receptors. J. Ethnopharmacol. 2019, 232, 39–46. [Google Scholar] [CrossRef]
- Zhang, J.C.; Zhang, Z.M.; Zeng, X.P.; Liu, L.; Zhou, X.W.; Zhang, Q.H.; Lin, C.H. Guizhou Real Estate Uncaria rhynchophylla Root Zone and Non Root Zone Soil Carbon, Nitrogen and Phosphorus Distribution. J. Chin. Med. Mater. 2017, 40, 2491–2495. (In Chinese) [Google Scholar] [CrossRef]
- Zeng, X.P.; Zhang, Z.M.; Sun, C.; Hong, J. Characteristics and influencing factors of soil enzyme activity of rhynchus Uncaria in Guizhou Province under different land use patterns. J. Chin. Med. Mater. 2019, 42, 970–973. (In Chinese) [Google Scholar] [CrossRef]
- Yang, Z.H.; Zhu, Y.D.; Yang, B.S.; Wang, H.; Li, F.; Jiao, K.Q.; Qin, G.H. The physiological and ecological responses of two species of ectomycorrhizal fungi to Cd stress. Chin. J. Environ. Eng. 2022, 41, 1–14. (In Chinese) [Google Scholar]
- Zhang, J.C.; Zeng, X.P.; Zhang, Z.M.; Lin, S.X.; Zhang, Q.H.; Lin, C.H. Evaluation and cumulative characteristics of heavy metals in soil-Uncaria rhynchophylla system of different functional areas. China J. Chin. Mater. Med. 2016, 41, 3746–3752. (In Chinese) [Google Scholar] [CrossRef]
- Dang, H.M.; Zhao, H.; He, T.B.; Lin, C.H.; Zhao, L.Y. Safety evaluation of heavy metals in the soil of rhynchus Uncaria with different planting years in Guizhou. J. Chin. Med. Mater. 2015, 38, 2257–2261. (In Chinese) [Google Scholar] [CrossRef]
- Lin, S.X.; Zeng, X.P.; Lin, C.H.; Xiao, Z.Q. Research on Yellow Soil Properties with Uncaria rhynchophylla-plant in Guizhou. Chin. Agric. Sci. Bull. 2020, 36, 118–123. (In Chinese) [Google Scholar]
- Zhao, H.; He, T.B.; Lin, C.H.; Zhao, L.Y.; Zeng, X.P.; Fan, B.; Liu, X.L. Research on Nutrients and Enzyme Activities in Uncaria rhynchophylla—Grown Soil at Different Habitats in Guizhou. J. Mt. Agric. Biol. 2014, 33, 23–26+50. (In Chinese) [Google Scholar] [CrossRef]
- Zeng, X.P.; Lin, S.X.; He, T.B.; Fan, B.; Zhao, H.; Zhao, L.Y.; Li, L.L.; Lin, C.H. Charateristics of nutrient content in soil in uncaria origins from Guizhou. GuangDong Agric. Sci. 2014, 41, 89–92. (In Chinese) [Google Scholar] [CrossRef]
- Ahmad, M.; Muhammad, D.; Mussarat, M.; Khan, A.A.; Khan, S.F.; Javed, M.W. Appraisal for site specific plant nutrient management through spatial variability and mapping in hilly areas of northern Pakistan. J. Soils Sediments 2017, 17, 936–948. [Google Scholar] [CrossRef]
- Arévalo-Gardini, E.; Canto, M.; Alegre, J.; Arévalo-Hernández, C.O.; Loli, O.; Julca, A.; Baligar, V. Cacao agroforestry management systems effects on soil fungi diversity in the Peruvian Amazon. Ecol. Indic. 2020, 115, 106404. [Google Scholar] [CrossRef]
- Shao, H.; Wang, H.; Wang, Y.; Xu, H.F.; Su, Q.; Liu, Y.G. Effects of different land use modes on soil fertility and heavy metal contents in karst rocky desertification area. J. Zhejiang AF Univ. 2022, 39, 635–643. (In Chinese) [Google Scholar] [CrossRef]
- Giller, K.E.; Witter, E.; McGrath, S.P. Heavy metals and soil microbes. Soil Biol. Biochem. 2009, 41, 2031–2037. [Google Scholar] [CrossRef]
- Xian, Y.; Wang, M.; Chen, W. Quantitative assessment on soil enzyme activities of heavy metal contaminated soils with various soil properties. Chemosphere 2015, 139, 604–608. [Google Scholar] [CrossRef]
- Zhang, Y.; Zhang, X.; Bi, Z.; Yu, Y.; Shi, P.; Ren, L.; Shan, Z. The impact of land use changes and erosion process on heavy metal distribution in the hilly area of the Loess Plateau, China. Sci. Total Environ. 2020, 718, 137305. [Google Scholar] [CrossRef] [PubMed]
- Shi, P.; Zhang, Y.; Zhang, Y.; Yu, Y.; Li, P.; Li, Z.; Xiao, L.; Xu, G.; Zhu, T. Land-use types and slope topography affect the soil labile carbon fractions in the Loess hilly-gully area of Shaanxi, China. Arch. Agron. Soil Sci. 2020, 66, 638–650. [Google Scholar] [CrossRef]
- Jarsjö, J.; Chalov, S.R.; Pietroń, J.; Alekseenko, A.V.; Thorslund, J. Patterns of soil contamination, erosion and river loading of metals in a gold mining region of northern Mongolia. Reg. Environ. Chang. 2017, 17, 1991–2005. [Google Scholar] [CrossRef]
- Wang, Z.; Xiao, J.; Wang, L.; Liang, T.; Guo, Q.; Guan, Y.; Rinklebe, J. Elucidating the differentiation of soil heavy metals under different land uses with geographically weighted regression and self-organizing map. Environ. Pollut. 2020, 260, 114065. [Google Scholar] [CrossRef] [PubMed]
- Sun, H.; Tan, C.Y.; Huang, D.Y.; Wan, D.J.; Liu, L.K.; Yang, Y.; Yu, X. Effects of Soil Organic Matter on the Accumulation, Availability and Chemical Speciation of Heavy Metal. J. Nat. Sci. Hunan Norm. Univ. 2011, 34, 82–87. (In Chinese) [Google Scholar]
- Mao, C.; Song, Y.; Chen, L.; Ji, J.; Li, J.; Yuan, X.; Yang, Z.; Ayoko, G.A.; Frost, R.L.; Theiss, F. Human health risks of heavy metals in paddy rice based on transfer characteristics of heavy metals from soil to rice. Catena 2019, 175, 339–348. [Google Scholar] [CrossRef]
- Wang, F.P.; Xiao, N.C.; Zhou, L.; Pang, R.; Song, B. Spatial Distribution Characteristics and Pollution Assessment of Heavy Metals on Farmland of Geochemical Anomaly Area in Southwest Guangxi. Environ. Sci. 2020, 41, 876–885. (In Chinese) [Google Scholar] [CrossRef]
- Gong, J.; Hou, W.; Liu, J.; Malik, K.; Kong, X.; Wang, L.; Chen, X.; Tang, M.; Zhu, R.; Cheng, C. Effects of Different Land Use Types and Soil Depths on Soil Mineral Elements, Soil Enzyme Activity, and Fungal Community in Karst Area of Southwest China. Int. J. Environ. Res. Public. Health 2022, 19, 3120. [Google Scholar] [CrossRef]
- Cang, L.; Zhou, D.-M.; Wang, Q.-Y.; Wu, D.-Y. Effects of electrokinetic treatment of a heavy metal contaminated soil on soil enzyme activities. J. Hazard. Mater. 2009, 172, 1602–1607. [Google Scholar] [CrossRef]
- Kaisa, W.; Rita, H.; Mikkonen, A.; Lappi, K.; Lindström, K.; Hartikainen, H.; Raateland, A.; Niemi, R.M. Effects of land use on the level, variation and spatial structure of soil enzyme activities and bacterial communities. Soil Biol. Biochem. 2011, 43, 1464–1473. [Google Scholar] [CrossRef]
- Tang, B.; Xu, H.; Song, F.; Ge, H.; Yue, S. Effects of heavy metals on microorganisms and enzymes in soils of lead–zinc tailing ponds. Environ. Res. 2022, 207, 112174. [Google Scholar] [CrossRef] [PubMed]
- Zheng, Y.-G.; Zhang, Z.-G.; Yao, D.-X.; Chen, X.-Y. Characteristics of temporal spatial distribution and enrichment of heavy metals in coal mine reclaimed soil. J. China Coal Soc. 2013, 38, 1476–1483. [Google Scholar]
- Li, M.; Zhang, X.; Lu, J. Application of fuzzy comprehensive assessment in pollution assessment of heavy mental in farmland soil. Southwest China J. Agric. Sci. 2010, 23, 1581–1585. [Google Scholar]
- Nie, J.R.; Ma, Y.H.; Xu, L.L.; Fu, H.H.; Ma, T.Z. Discussion About Heavy Metal Pollution in Soil Environmental Quality Standard in China. J. Agric. Resour. Environ. 2013, 30, 44–49. (In Chinese) [Google Scholar] [CrossRef]
- He, W.X.; Zhu, M.E.; Zhang, Y.P. Recent Advance in Relationship between Soil Enzymes and Heavy Metals. Soil Environ. Sci. 2000, 9, 139–142. (In Chinese) [Google Scholar]
- Zhou, J.; Li, H.; Li, X.L.; Wang, H.C.; Jin, C.; Nan, Z.G. The relationship between forms of Cd and soil enzymatic activities in sludge-amended soil. Environ. Chem. 2016, 35, 2036–2043. (In Chinese) [Google Scholar] [CrossRef]
- Hinojosa, M.B.; García-Ruíz, R.; Viñegla, B.; Carreira, J.A. Microbiological rates and enzyme activities as indicators of functionality in soils affected by the Aznalcóllar toxic spill. Soil Biol. Biochem. 2004, 36, 1637–1644. [Google Scholar] [CrossRef]
- Moghimian, N.; Hosseini, S.M.; Kooch, Y.; Darki, B.Z. Impacts of changes in land use/cover on soil microbial and enzyme activities. Catena 2017, 157, 407–414. [Google Scholar] [CrossRef]
Land Use Type | pH | Organic Matter (g/kg) | Available N (mg/kg) | Available Phosphorus (mg/kg) | Available K (mg/kg) |
---|---|---|---|---|---|
Uncaria rhynchophylla base | 5.95 | 23.58 | 103.36 | 5.62 | 79.39 |
Forestland | 5.72 | 22.03 | 105.34 | 8.09 | 107.12 |
Wasteland | 6.17 | 14.26 | 80.36 | 5.62 | 77.05 |
Index | Project | Determination Method |
---|---|---|
Physical index | pH | Potentiometric method, water and soil ratio is 1:2.5 |
Chemical index | Organic matter | Potassium dichromate volumetric method external heating method |
Available N | Alkali hydrolysis diffusion method | |
Available phosphorus | Keywords hydrochloric acid ammonia fluoride extraction molybdenum antimony anti colorimetric method | |
Available K | Ammonium acetate extraction flame spectrophotometry | |
Heavy metal | Cu, As, Cd, Pb, Hg, Cr | Three acid digestion and ICP-MS determination |
Enzymatic activity | Catalase | Potassium permanganate titration |
Acid phosphatase | Colorimetric method of disodium phenylphosphate | |
Urease | Indophenol blue colorimetry |
Classification | Single Pollution Index Pi | Nemero Synthesis Pollution Index Pn | Pollution Level |
---|---|---|---|
Ⅰ | Pi ≤ 0.7 | Pn ≤ 0.7 | Cleaning (Safety) |
Ⅱ | 0.7 < Pi ≤ 1 | 0.7 < Pn ≤ 1 | Still clean (Warning line) |
Ⅲ | 1 < Pi ≤ 2 | 1 < Pn ≤ 2 | Light pollution (Crops begin to be polluted) |
Ⅳ | 2 < Pi ≤ 3 | 2 < Pn ≤ 3 | Moderate pollution (Heavy pollution of crops) |
Ⅴ | Pi > 3 | Pn > 3 | Heavy pollution (Crops are seriously polluted) |
Land Use Type | Descriptive Statistics | Cu | As | Cd | Hg | Pb | Cr |
---|---|---|---|---|---|---|---|
Uncaria rhynchophylla base | Range | 0.73~19.72 | 0.50~8.95 | 0~0.46 | 0~0.38 | 14.41~30.24 | 13.38~56.73 |
Average value | 9.16 | 3.36 | 0.30 | 0.12 | 21.01 | 35.48 | |
SD | 5.26 | 2.39 | 0.12 | 0.14 | 5.06 | 15.97 | |
CV (%) | 57.39 | 70.91 | 41.77 | 110.86 | 24.1 | 45.01 | |
Forestland | Range | 2.12~5.38 | 2.02~3.43 | 0.29~0.45 | 0.02~0.11 | 17.49~28.33 | 24.59~28.53 |
Average value | 4.16 | 2.94 | 0.35 | 0.06 | 20.97 | 26.24 | |
SD | 1.25 | 0.56 | 0.07 | 0.03 | 4.4 | 1.57 | |
CV (%) | 30.05 | 19.04 | 20.00 | 50.00 | 20.98 | 5.98 | |
Wasteland | Range | 4.57~6.5 | 1.31~1.71 | 0.11~0.25 | 0~0.19 | 21.2~25.92 | 26.25~27.9 |
Average value | 5.57 | 1.50 | 0.20 | 0.07 | 23.92 | 27.16 | |
SD | 0.97 | 0.2 | 0.08 | 0.11 | 2.44 | 0.84 | |
CV (%) | 17.34 | 13.31 | 39.97 | 153.09 | 10.19 | 3.1 | |
National Soil Background Values | 22.6 | 11.2 | 0.097 | 0.065 | 26.00 | 61.0 | |
Soil background value in Guizhou Province | 32.00 | 20.00 | 0.659 | 0.110 | 35.20 | 95.90 | |
Environmental quality of green food origin | 50.00 | 25.00 | 0.30 | 0.25 | 50.00 | 120.00 |
Land Use Type | Pi | Pn | |||||
---|---|---|---|---|---|---|---|
Cu | As | Cd | Hg | Pb | Cr | ||
Uncaria rhynchophylla base | 0.183 | 0.084 | 1.000 | 0.067 | 0.233 | 0.237 | 0.738 |
Forestland | 0.104 | 0.074 | 1.167 | 0.033 | 0.233 | 0.175 | 0.850 |
Wasteland | 0.111 | 0.038 | 0.667 | 0.039 | 0.266 | 0.181 | 0.504 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Yang, X.; Zhang, Z.; Sun, C.; Zeng, X. Soil Heavy Metal Content and Enzyme Activity in Uncaria rhynchophylla-Producing Areas under Different Land Use Patterns. Int. J. Environ. Res. Public Health 2022, 19, 12220. https://doi.org/10.3390/ijerph191912220
Yang X, Zhang Z, Sun C, Zeng X. Soil Heavy Metal Content and Enzyme Activity in Uncaria rhynchophylla-Producing Areas under Different Land Use Patterns. International Journal of Environmental Research and Public Health. 2022; 19(19):12220. https://doi.org/10.3390/ijerph191912220
Chicago/Turabian StyleYang, Xiuyuan, Zhenming Zhang, Chao Sun, and Xianping Zeng. 2022. "Soil Heavy Metal Content and Enzyme Activity in Uncaria rhynchophylla-Producing Areas under Different Land Use Patterns" International Journal of Environmental Research and Public Health 19, no. 19: 12220. https://doi.org/10.3390/ijerph191912220