Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice

Lawrence, Marcus M.; Zwetsloot, Kevin A.; Arthur, Susan T.; Sherman, Chase A.; Huot, Joshua R.; Badmaev, Vladimir; Grace, Mary; Lila, Mary Ann; Nieman, David C.; Shanely, R. Andrew

doi:10.3390/ijerph18020370

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Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice

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R. Andrew Shanely

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Department of Health and Exercise Science, Appalachian State University, Boone, NC 28608, USA

Human Performance Laboratory, North Carolina Research Campus, Kannapolis, NC 28081, USA

Integrated Muscle Physiology Laboratory, Boone, NC 28607, USA

⁴

Department of Kinesiology and Outdoor Recreation, Southern Utah University, Cedar City, UT 84720, USA

⁵

Laboratory of Systems Physiology, Department of Kinesiology, University of North Carolina Charlotte, Charlotte, NC 28223, USA

⁶

Department of Biology, Appalachian State University, Boone, NC 20608, USA

⁷

American Medical Holdings Inc., Staten Island, NY 10314, USA

⁸

Plants for Human Health Institute, North Carolina Research Campus, North Carolina State University, Kannapolis, NC 28081, USA

Author to whom correspondence should be addressed.

Int. J. Environ. Res. Public Health 2021, 18(2), 370; https://doi.org/10.3390/ijerph18020370

Received: 7 December 2020 / Revised: 28 December 2020 / Accepted: 30 December 2020 / Published: 6 January 2021

(This article belongs to the Special Issue Regulation of Muscle Mass, Exercise, Metabolism)

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Abstract

Skeletal muscle mass and strength are lost with aging. Phytoecdysteroids, in particular 20-hydroxyecdysone (20E), increase protein synthesis in C2C12 skeletal muscle cells and muscle strength in young rats. The objective of this study was to determine whether an extract from Ajuga turkestanica (ATE), enriched in phytoecdysteroids, and 20E affect skeletal muscle mass and fiber size, fiber type, activation of the PI3K–Akt signaling pathway, and the mRNA levels of MAFbx, MuRF-1, and myostatin in sedentary aging mice. Aging male C57BL/6 mice (20 months old) received ATE, 20E, or vehicle (CT) once per day for 28 days or a single acute dose. Treatment did not alter body, muscle, or organ mass; fiber cross-sectional area; or fiber type in the triceps brachii or plantaris muscles. Likewise, protein synthesis signaling markers (i.e., phosphorylation of Akt^Ser473 and p70S6k^Thr389) measured after either 28 days or acutely were unchanged. Neither ATE nor 20E treatment for 28 days affected the mRNA levels of MAFbx, MuRF-1, and myostatin. In conclusion, these data indicate that phytoecdysteroid treatment does not alter muscle mass or fiber type, nor does it activate protein synthesis signaling in the skeletal muscle of sedentary aging mice. View Full-Text

Keywords: skeletal muscle; hypertrophy; Akt; p70S6K; atrogenes; 20-hydroxyecdysone

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Lawrence, M.M.; Zwetsloot, K.A.; Arthur, S.T.; Sherman, C.A.; Huot, J.R.; Badmaev, V.; Grace, M.; Lila, M.A.; Nieman, D.C.; Shanely, R.A. Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice. Int. J. Environ. Res. Public Health 2021, 18, 370. https://doi.org/10.3390/ijerph18020370

AMA Style

Lawrence MM, Zwetsloot KA, Arthur ST, Sherman CA, Huot JR, Badmaev V, Grace M, Lila MA, Nieman DC, Shanely RA. Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice. International Journal of Environmental Research and Public Health. 2021; 18(2):370. https://doi.org/10.3390/ijerph18020370

Chicago/Turabian Style

Lawrence, Marcus M., Kevin A. Zwetsloot, Susan T. Arthur, Chase A. Sherman, Joshua R. Huot, Vladimir Badmaev, Mary Grace, Mary A. Lila, David C. Nieman, and R. A. Shanely. 2021. "Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice" International Journal of Environmental Research and Public Health 18, no. 2: 370. https://doi.org/10.3390/ijerph18020370

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Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice

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