Antitumor and Antioxidant Activity of S-Methyl Methionine Sulfonium Chloride against Liver Cancer Induced in Wistar Albino Rats by Diethyl Nitrosamine and Carbon Tertrachloride
Abstract
:1. Introduction
2. Material and Methods
2.1. Material
2.2. Animals
2.3. Preparation of DEN and CCl4
2.4. Experimental Design
2.5. Blood Samples
2.6. Tissue Sample
2.7. Serum Biochemical Assays
2.8. Assessment of Hepaticoxidative/Antioxidant Status
2.9. Histopathological Examination
2.10. Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)
2.11. Data Statistical Analysis
3. Result
3.1. Biochemical Findings
3.2. Oxidative/Antioxidant Activity
3.3. Histopathogy Findings
3.4. Inflammatory Cytokines, and Immunoregulatory Cytokines Expression by qRT-PCR
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sense | Antisense | Annealing Temperature | |
---|---|---|---|
TNF-α | GACCCTCACACTCAGATCATCTTCT | TTGTCTTTGAGATCCATGCCATT | 60 |
iNOS | TCTTCAAGGACCTACCTCAGGC | GCTAAGGCAAAGCTGCTAGGTC | 60 |
TGF B-1 | TCACTTGTTTTGGTGGATGC | TTCTGTCTCTCAAGTCCCCC | 60 |
GP3 | GTGCTGGAACGGACAAGAG | TTCTTCATCCCATTCCTTGC | 60 |
β-actin | TGTTGTCCCTGTATGCCTCT | TAATGTCACGCACGATTTCC | 60 |
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Abouzed, T.K.; Althobaiti, F.; Abdelkhlek, N.A.; Eldomany, E.B.; Nasr, N.E.; Sadek, K.M.; El-Shazly, S.A.; Kahilo, K.A.; Dorghamm, D.A. Antitumor and Antioxidant Activity of S-Methyl Methionine Sulfonium Chloride against Liver Cancer Induced in Wistar Albino Rats by Diethyl Nitrosamine and Carbon Tertrachloride. Int. J. Environ. Res. Public Health 2021, 18, 9726. https://doi.org/10.3390/ijerph18189726
Abouzed TK, Althobaiti F, Abdelkhlek NA, Eldomany EB, Nasr NE, Sadek KM, El-Shazly SA, Kahilo KA, Dorghamm DA. Antitumor and Antioxidant Activity of S-Methyl Methionine Sulfonium Chloride against Liver Cancer Induced in Wistar Albino Rats by Diethyl Nitrosamine and Carbon Tertrachloride. International Journal of Environmental Research and Public Health. 2021; 18(18):9726. https://doi.org/10.3390/ijerph18189726
Chicago/Turabian StyleAbouzed, Tarek Kamal, Fayez Althobaiti, Nesreen Adel Abdelkhlek, Ehab Bedir Eldomany, Nasr Elsayed Nasr, Kadry Mohamed Sadek, Samir Ahmed El-Shazly, Khaled A. Kahilo, and Doaa Abdallha Dorghamm. 2021. "Antitumor and Antioxidant Activity of S-Methyl Methionine Sulfonium Chloride against Liver Cancer Induced in Wistar Albino Rats by Diethyl Nitrosamine and Carbon Tertrachloride" International Journal of Environmental Research and Public Health 18, no. 18: 9726. https://doi.org/10.3390/ijerph18189726