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Article

A Miniaturized Microbe-Silicon-Chip Based on Bioluminescent Engineered Escherichia coli for the Evaluation of Water Quality and Safety

1
Azienda Ospedaliero Universitaria Policlinico “G. Rodolico-San Marco”, Via S. Sofia 78, 95123 Catania, Italy
2
Istituto per la Microelettronica e Microsistemi–Consiglio Nazionale delle Ricerche (CNR-IMM), Ottava Strada 5, 95121 Catania, Italy
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Department of Fundamental Microbiology, Bâtiment Biophore, University of Lausanne, 1015 Lausanne, Switzerland
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Azienda Sanitaria Provinciale di Catania, Via S. Maria La Grande 5, 95124 Catania, Italy
5
Regional Reference Laboratory of Clinical and Environmental Surveillance of Legionellosis, Catania, Department of Medical and Surgical Sciences and Advanced Technologies “G.F. Ingrassia”, University of Catania, Via Sofia 87, 95123 Catania, Italy
*
Authors to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Int. J. Environ. Res. Public Health 2021, 18(14), 7580; https://doi.org/10.3390/ijerph18147580
Received: 16 June 2021 / Revised: 14 July 2021 / Accepted: 15 July 2021 / Published: 16 July 2021
Conventional high throughput methods assaying the chemical state of water and the risk of heavy metal accumulation share common constraints of long and expensive analytical procedures and dedicated laboratories due to the typical bulky instrumentation. To overcome these limitations, a miniaturized optical system for the detection and quantification of inorganic mercury (Hg2+) in water was developed. Combining the bioactivity of a light-emitting mercury-specific engineered Escherichia coli—used as sensing element—with the optical performance of small size and inexpensive Silicon Photomultiplier (SiPM)—used as detector—the system is able to detect mercury in low volumes of water down to the concentration of 1 µg L−1, which is the tolerance value indicated by the World Health Organization (WHO), providing a highly sensitive and miniaturized tool for in situ water quality analysis. View Full-Text
Keywords: water analysis; engineered Escherichia coli; miniaturizable optical system; mercury sensing; SiPM water analysis; engineered Escherichia coli; miniaturizable optical system; mercury sensing; SiPM
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MDPI and ACS Style

Sciuto, E.L.; Corso, D.; Libertino, S.; van der Meer, J.R.; Faro, G.; Coniglio, M.A. A Miniaturized Microbe-Silicon-Chip Based on Bioluminescent Engineered Escherichia coli for the Evaluation of Water Quality and Safety. Int. J. Environ. Res. Public Health 2021, 18, 7580. https://doi.org/10.3390/ijerph18147580

AMA Style

Sciuto EL, Corso D, Libertino S, van der Meer JR, Faro G, Coniglio MA. A Miniaturized Microbe-Silicon-Chip Based on Bioluminescent Engineered Escherichia coli for the Evaluation of Water Quality and Safety. International Journal of Environmental Research and Public Health. 2021; 18(14):7580. https://doi.org/10.3390/ijerph18147580

Chicago/Turabian Style

Sciuto, Emanuele Luigi, Domenico Corso, Sebania Libertino, Jan Roelof van der Meer, Giuseppina Faro, and Maria Anna Coniglio. 2021. "A Miniaturized Microbe-Silicon-Chip Based on Bioluminescent Engineered Escherichia coli for the Evaluation of Water Quality and Safety" International Journal of Environmental Research and Public Health 18, no. 14: 7580. https://doi.org/10.3390/ijerph18147580

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