Comparison of Liquid Chromatography Mass Spectrometry and Enzyme-Linked Immunosorbent Assay Methods to Measure Salivary Cotinine Levels in Ill Children
Abstract
1. Introduction
2. Materials and Methods
2.1. Subjects and Biological Samples
2.2. Measures
2.3. Chemical Analyses
2.4. Statistical Analyses
2.4.1. Mixed Model
2.4.2. Paired Sample t-Test
2.4.3. Linear Regression Models
3. Results
3.1. Demographics and TSE Patterns
3.2. Cotinine Measurements and Comparisons of Analyses by LC-MS/MS and ELISA Distribution of Cotinine by LC-MS/MS Compared to ELISA
3.3. Internal Consistency of LC-MS/MS and ELISA
3.4. Differences in Cotinine Results by Demographics, TSE, and Home Characteristics
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Analyte | Method | Total Samples Analyzed | Total Samples Detected | Percent Detected | LOQ | GeoM | Geometric SD | Min | P25 | P50 | P75 | P95 | Max |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cotinine (ng/mL) | LC-MS/MS | 218 | 211 | 97% | 0.10 | 4.1 | 4.1 | <LOQ | 1.3 | 4.3 | 10.3 | 41.5 | 382 |
ELISA | 218 | 208 | 95% | 0.15 | 5.7 | 3.5 | <LOQ | 2.2 | 5.1 | 12.2 | 41.2 | 364 |
Internal ELISA (N = 55) | Internal LC-MS/MS (N = 20) | |||||
---|---|---|---|---|---|---|
Across methods (N = 203) | ICC | Median RPD * | ICC | Median RPD | ||
Overall | 0.884 | 0.993 | 0.113 | 0.991 | 0.061 | |
Age | 0–6 yrs (n = 122) | 0.899 | 0.992 | 0.118 | 0.996 | 0.057 |
7–17 yrs (n = 81) | 0.824 | 0.994 | 0.099 | 0.983 | 0.073 | |
Sex | Male (n = 100) | 0.890 | 0.993 | 0.087 | 0.995 | 0.056 |
Female (n = 103) | 0.869 | 0.993 | 0.156 | 0.985 | 0.073 |
LC-MS/MS Based Cotinine | ELISA Based Cotinine | ||||||||
---|---|---|---|---|---|---|---|---|---|
Parameter | % of Cohort | 95% LCL | 95% UCL | p-Value | 95% LCL | 95% UCL | p-Value | ||
Age of child | N/A | −0.11 | −0.15 | −0.08 | < 0.0001 | −0.09 | −0.13 | −0.06 | < 0.0001 |
# cigarettes/day smoked-caregiver | N/A | 0.09 | 0.05 | 0.12 | < 0.0001 | 0.07 | 0.04 | 0.11 | < 0.0001 |
# cigarettes/day smoked around child | N/A | 0.01 | −0.00 | 0.02 | 0.17 | 0.01 | −0.01 | 0.02 | 0.34 |
Sex of child | |||||||||
Female | 51.3% | Ref | Ref | Ref | Ref | Ref | Ref | Ref | Ref |
Male | 48.7% | 0.38 | 0.02 | 0.75 | 0.04 | 0.30 | −0.06 | 0.65 | 0.10 |
Race/ethnicity | |||||||||
Non-Hispanic White | 36.6% | Ref | Ref | Ref | Ref | Ref | Ref | Ref | Ref |
Non-Hispanic Black | 54.3% | 0.52 | 0.10 | 0.95 | 0.04 | 0.38 | −0.03 | 0.80 | 0.16 |
Other | 9.1% | 0.07 | −0.61 | 0.75 | 0.04 | 0.06 | −0.61 | 0.73 | 0.16 |
Caregiver’s Annual Income | |||||||||
Less than $5,000 | 35.5% | Ref | Ref | Ref | Ref | Ref | Ref | Ref | Ref |
$5,001 to $15,000 | 26.9% | 0.10 | −0.35 | 0.55 | 0.01 | 0.04 | −0.40 | 0.47 | 0.02 |
$15,001 to $30,000 | 21.3% | −0.44 | −0.93 | 0.05 | 0.01 | −0.49 | −0.97 | −0.01 | 0.02 |
$30,001 to $50,000 | 9.1% | −1.07 | −1.74 | −0.41 | 0.01 | −0.91 | −1.56 | −0.27 | 0.02 |
$50,001 to $75,000 | 5.6% | −0.48 | −1.29 | 0.34 | 0.01 | −0.43 | −1.23 | 0.36 | 0.02 |
More than $75,000 | 1.5% | −0.98 | −2.50 | 0.55 | 0.01 | −1.55 | −3.04 | −0.06 | 0.02 |
Type of home | |||||||||
Single family house | 48.2% | Ref | Ref | Ref | Ref | Ref | Ref | Ref | Ref |
Multi-family house | 20.3% | 0.49 | 0.02 | 0.96 | 0.06 | 0.22 | −0.24 | 0.68 | 0.19 |
Apartment | 30.0% | 0.53 | 0.10 | 0.97 | 0.06 | 0.45 | 0.02 | 0.87 | 0.19 |
Other | 1.5% | 0.66 | −0.82 | 2.14 | 0.06 | −0.37 | −1.81 | 1.08 | 0.19 |
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Mahabee-Gittens, E.M.; Mazzella, M.J.; Doucette, J.T.; Merianos, A.L.; Stone, L.; Wullenweber, C.A.; A. Busgang, S.; Matt, G.E. Comparison of Liquid Chromatography Mass Spectrometry and Enzyme-Linked Immunosorbent Assay Methods to Measure Salivary Cotinine Levels in Ill Children. Int. J. Environ. Res. Public Health 2020, 17, 1157. https://doi.org/10.3390/ijerph17041157
Mahabee-Gittens EM, Mazzella MJ, Doucette JT, Merianos AL, Stone L, Wullenweber CA, A. Busgang S, Matt GE. Comparison of Liquid Chromatography Mass Spectrometry and Enzyme-Linked Immunosorbent Assay Methods to Measure Salivary Cotinine Levels in Ill Children. International Journal of Environmental Research and Public Health. 2020; 17(4):1157. https://doi.org/10.3390/ijerph17041157
Chicago/Turabian StyleMahabee-Gittens, E. Melinda, Matthew J. Mazzella, John T. Doucette, Ashley L. Merianos, Lara Stone, Chase A. Wullenweber, Stefanie A. Busgang, and Georg E. Matt. 2020. "Comparison of Liquid Chromatography Mass Spectrometry and Enzyme-Linked Immunosorbent Assay Methods to Measure Salivary Cotinine Levels in Ill Children" International Journal of Environmental Research and Public Health 17, no. 4: 1157. https://doi.org/10.3390/ijerph17041157
APA StyleMahabee-Gittens, E. M., Mazzella, M. J., Doucette, J. T., Merianos, A. L., Stone, L., Wullenweber, C. A., A. Busgang, S., & Matt, G. E. (2020). Comparison of Liquid Chromatography Mass Spectrometry and Enzyme-Linked Immunosorbent Assay Methods to Measure Salivary Cotinine Levels in Ill Children. International Journal of Environmental Research and Public Health, 17(4), 1157. https://doi.org/10.3390/ijerph17041157