Oral Microbiome of Children Living in an Isolated Area in Myanmar
Department of Translational Research, Tsurumi University School of Dental Medicine, Yokohama 230-8501, Japan
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Int. J. Environ. Res. Public Health 2020, 17(11), 4033; https://doi.org/10.3390/ijerph17114033
Received: 5 May 2020
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Revised: 2 June 2020
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Accepted: 3 June 2020
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Published: 5 June 2020
(This article belongs to the Special Issue Community Dental Care)
Several studies have shown that the oral microbiome is related to systemic health, and a co-relation with several specific diseases has been suggested. The oral microbiome depends on environmental- and community-level factors. In this observational study, the oral microbiomes of children of isolated mountain people were analyzed with respect to the core oral microbiome and etiology of dental caries. We collected samples of supragingival plaque from children (age 9–13) living in the Chin state of Myanmar. After DNA extraction and purification, next-generation sequencing of the V3–V4 hypervariable regions of the 16S rRNA was conducted. From thirteen subjects, 263,458 valid reads and 640 operational taxonomic units were generated at a 97% identity cut-off value. At the phylum level, Proteobacteria was the most abundant, followed by Firmicutes and Bacteroides. Forty-four bacteria were detected in total from all the subjects. For children without dental caries, Proteobacteria was abundant. In contrast, in children with dental caries, Firmicutes and Bacteroides were abundant. The oral microbiome of children living in an isolated area may be affected by environmental- and community-level factors. Additionally, the composition of the oral microbiome may affect the risk of dental caries.
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Keywords:
oral microbiome; dental caries; children
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MDPI and ACS Style
Nomura, Y.; Otsuka, R.; Hasegawa, R.; Hanada, N. Oral Microbiome of Children Living in an Isolated Area in Myanmar. Int. J. Environ. Res. Public Health 2020, 17, 4033. https://doi.org/10.3390/ijerph17114033
AMA Style
Nomura Y, Otsuka R, Hasegawa R, Hanada N. Oral Microbiome of Children Living in an Isolated Area in Myanmar. International Journal of Environmental Research and Public Health. 2020; 17(11):4033. https://doi.org/10.3390/ijerph17114033
Chicago/Turabian StyleNomura, Yoshiaki, Ryoko Otsuka, Ryo Hasegawa, and Nobuhiro Hanada. 2020. "Oral Microbiome of Children Living in an Isolated Area in Myanmar" International Journal of Environmental Research and Public Health 17, no. 11: 4033. https://doi.org/10.3390/ijerph17114033
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