Next Article in Journal
Lung Cancer Mortality Trends in a Brazilian City with a Long History of Asbestos Consumption
Next Article in Special Issue
Concentrations of Atmospheric Culturable Bioaerosols at Mountain and Seashore Sites
Previous Article in Journal
Arterial Stiffness Parameters Correlate with Estimated Cardiovascular Risk in Humans: A Clinical Study
Previous Article in Special Issue
Major Differences in the Diversity of Mycobiomes Associated with Wheat Processing and Domestic Environments: Significant Findings from High-Throughput Sequencing of Fungal Barcode ITS1
Open AccessArticle

Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods

Department of Public Health and Paediatrics, University of Torino, Piazza Polonia 94, 10126 Torino, Italy
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2019, 16(14), 2546; https://doi.org/10.3390/ijerph16142546
Received: 30 May 2019 / Revised: 11 July 2019 / Accepted: 11 July 2019 / Published: 17 July 2019
(This article belongs to the Special Issue Environmental and Occupational Exposure to Microbial Contaminants)
Bioaerosol exposure linked to the bioenergy production from waste and its effects on human health in occupational and residential environments has rising interest nowadays. The health risk associated with the exposure includes mainly infective diseases, allergies, chronic bronchitis, and obstructive pulmonary disease. A risk assessment’s critical point is the bioaerosol quality and quantity characterization. The aim of this study is to evaluate the application of different methods for the analysis of bioaerosol sampled in bioenergy plants. This study involved six Italian plants for the treatment of biomasses and energy production. Bioaerosol cultural evaluation was performed, by Surface Air System (SAS) sampler, and DNA was extracted from PM0.49 samples and Low Melting Agar plates. qRT-PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE) and band sequencings were performed. The cultural method is able to detect less than 15% of what is evaluable with bio-molecular methods. A low sample alfa-diversity and a high beta-biodiversity in relation to feedstock and process were observed. Sequencing showed microorganisms with a hygienic-sanitary relevance such as Arcobacter, Pseudomonas, Enterobacter, Klebsiella, Enterococcus and Bacillus. Integrated cultural and biomolecular methods can be more exhaustive to evaluate bioaerosol’s exposure in the occupational environment. View Full-Text
Keywords: bioaerosol; qRT-PCR; PCR-DGGE; microbial pollution; bioenergy production bioaerosol; qRT-PCR; PCR-DGGE; microbial pollution; bioenergy production
Show Figures

Graphical abstract

MDPI and ACS Style

Anedda, E.; Carletto, G.; Gilli, G.; Traversi, D. Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods. Int. J. Environ. Res. Public Health 2019, 16, 2546.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop