Next Article in Journal
Chronic Manganese Toxicity Associated with Voltage-Gated Potassium Channel Complex Antibodies in a Relapsing Neuropsychiatric Disorder
Next Article in Special Issue
A Simulation-Based Study on the Comparison of Statistical and Time Series Forecasting Methods for Early Detection of Infectious Disease Outbreaks
Previous Article in Journal
Mapping European Welfare Models: State of the Art of Strategies for Professional Integration and Reintegration of Persons with Chronic Diseases
Previous Article in Special Issue
The Role of Culex pipiens L. (Diptera: Culicidae) in Virus Transmission in Europe
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Int. J. Environ. Res. Public Health 2018, 15(4), 782; https://doi.org/10.3390/ijerph15040782

The Epidemiological Significance and Temporal Stability of Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats-Based Method Applied to Mycobacterium tuberculosis in China

1
Department of Epidemiology, China and Key Laboratory of Public Health Safety (Fudan University), School of Public Health, Fudan University, Ministry of Education, Shanghai 200032, China
2
Department of Public Health Sciences, Karolinska Institutet, SE-171 77 Stockholm, Sweden
3
The Public Health Agency of Sweden, SE-171 82 Solna, Sweden
*
Author to whom correspondence should be addressed.
Received: 8 March 2018 / Revised: 11 April 2018 / Accepted: 11 April 2018 / Published: 17 April 2018
(This article belongs to the Special Issue Emerging Infectious Disease (EID) Research, Management and Response)
Full-Text   |   PDF [759 KB, uploaded 3 May 2018]   |  

Abstract

This study aimed to validate the epidemiological significance and temporal stability of Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) typing in a genetically and geographically diverse set of clinical isolates from patients diagnosed with pulmonary tuberculosis in China. Between 2010 and 2013, a total of 982 Mycobacterium tuberculosis isolates were collected from four population-based investigations in China. Apart from the currently applied 24-locus MIRU-VNTR, six additional hypervariable loci were analyzed in order to validate the MIRU-VNTR combinations in terms of their epidemiological links, clustering time span, and paired geographic distance. In vitro temporal stability was analyzed for both individual MIRU-VNTR loci, and for several combinations of loci. In the present study, four MIRU-VNTR combinations, including the hypervariable loci 3820, 3232, 2163a, and 4120, were evaluated. All of these combinations obtained a Hunter-Gaston discriminatory index (HGDI) value over 0.9900 with a reduced clustering proportion (from 32.0% to 25.6%). By comparing epidemiological links, clustering time span, and paired geographic distance, we found that the performances of the four MIRU-VNTR combinations were comparable to the insertion sequence 6110 restriction fragment length polymorphism (IS6110-RFLP), and significantly better than that of 24-locus MIRU-VNTR genotyping alone. The proportion of temporally stable loci ranged from 90.5% to 92.5% within the combined MIRU-VNTR genotyping, which is higher than IS6110-RFLP (85.4%). By adding four hypervariable loci to the standard 24-locus MIRU-VNTR genotyping, we obtained a high discriminatory power, stability and epidemiological significance. This algorithm could therefore be used to improve tuberculosis transmission surveillance and outbreak investigation in China. View Full-Text
Keywords: Mycobacterium tuberculosis; MIRU-VNTR; epidemiological significance; temporal stability; China Mycobacterium tuberculosis; MIRU-VNTR; epidemiological significance; temporal stability; China
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Li, Y.; Hu, Y.; Mansjö, M.; Zhao, Q.; Jiang, W.; Ghebremichael, S.; Hoffner, S.; Xu, B. The Epidemiological Significance and Temporal Stability of Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats-Based Method Applied to Mycobacterium tuberculosis in China. Int. J. Environ. Res. Public Health 2018, 15, 782.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top