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Int. J. Environ. Res. Public Health 2017, 14(7), 825; https://doi.org/10.3390/ijerph14070825

Effect of Patulin from Penicillium vulpinum on the Activity of Glutathione-S-Transferase and Selected Antioxidative Enzymes in Maize

1
Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
2
Institut für Botanik, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany
*
Author to whom correspondence should be addressed.
Received: 4 June 2017 / Revised: 11 July 2017 / Accepted: 18 July 2017 / Published: 22 July 2017
(This article belongs to the Special Issue Mycotoxins in the Agri-Food Chain)
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Abstract

The mycotoxin patulin (PAT) was purified from Penicillium vulpinum CM1 culture that has been isolated from a soil cultivated with maize. The effect of PAT and of a fungal culture filtrate on the activities of glutathione-S-transferase (GST) and some antioxidant enzymes viz. ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) was investigated in roots and shoots of 8-day-old maize seedlings. PAT and culture filtrate caused significant reduction effects in a dose-related manner on the total GST activity. Upon application of the high PAT concentration (25 μg·mL−1) and of the concentrated fungal filtrate (100%, v/v), the reduction in GST activity of roots was 73.8–76.0% and of shoots was 60–61.7%. Conversely, significant increases in the activities of antioxidant enzymes were induced. Application of 25 μg·PAT·mL−1 increased APX, GR, DHAR, and MDHAR activity of root by 2.40-, 2.00-, 1.24-, and 2.16-fold, respectively. In shoots, the enzymatic activity was increased by 1.57-, 1.45-, 1.45-, and 1.61-fold, respectively. Similar induction values of the enzymatic activity were obtained upon application of the concentrated fungal filtrate. This is the first report describing the response of GST and antioxidant enzyme activities of plant cells to PAT toxicity. View Full-Text
Keywords: glutathione-S-transferase (GST); antioxidants; maize; patulin (PAT); Penicillium vulpinum glutathione-S-transferase (GST); antioxidants; maize; patulin (PAT); Penicillium vulpinum
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Ismaiel, A.A.; Papenbrock, J. Effect of Patulin from Penicillium vulpinum on the Activity of Glutathione-S-Transferase and Selected Antioxidative Enzymes in Maize. Int. J. Environ. Res. Public Health 2017, 14, 825.

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