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Article

Overexpression of SKP2 Inhibits the Radiation-Induced Bystander Effects of Esophageal Carcinoma

by
Xiao-Chun Wang
1,
Tie-Jun Zhang
2,
Zi-Jian Guo
3,
Chang-Yan Xiao
1,
Xiao-Wen Ding
1,
Fang Fang
1,
Wen-Tao Sheng
1,
Xu Shu
4,* and
Jue Li
1,*
1
The Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry (Beijing Institute of Occupational Disease Prevention and Treatment), Beijing 100093, China
2
Department of Nuclear Medicine, The First Affiliated Hospital of Baotou Medical College, Baotou 014010, China
3
The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China
4
National Institute for Radiological Protection, China CDC, Beijing 100088, China
*
Authors to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2017, 14(2), 155; https://doi.org/10.3390/ijerph14020155
Submission received: 23 November 2016 / Revised: 19 January 2017 / Accepted: 31 January 2017 / Published: 6 February 2017
(This article belongs to the Section Global Health)
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Abstract

Background: To investigate the effects of S-phase kinase protein 2 (SKP2) expression on the radiation induced bystander effect (RIBE) in esophageal cancer (EC) cells. Materials and Methods: Western blot was used to detect the levels of SKP2, Rad51, and Ku70 in EC cells. Positive transfection, RNAi, micronucleus (MN), and γ-H2AX focus formation assay were used to investigate the effects of SKP2 on RIBE induced by irradiated cells. Results: We found a significant negative correlation between SKP2 expression and MN frequency (p < 0.05) induced by RIBE. The results were further confirmed by positive transfection, RNAi, and rescue experiments.γ-H2AX focus formation assay results indicated that overexpression of SKP2 in the irradiated cells inhibited the DNA damage of RIBE cells. However, when SKP2 expression decreased in irradiated cells, the DNA damage of RIBE cells increased. Increased or decreased expression levels of SKP2 had effects on Rad51 expression under the conditions of RIBE. Conclusions: These results showed, for the first time, that SKP2 expression can inhibit RIBE of EC cells. The mechanism may function, at least partly, through the regulation of Rad51 in the ability to repair DNA damage.
Keywords: SKP2; esophageal carcinoma; radiation-induced bystander effect SKP2; esophageal carcinoma; radiation-induced bystander effect

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MDPI and ACS Style

Wang, X.-C.; Zhang, T.-J.; Guo, Z.-J.; Xiao, C.-Y.; Ding, X.-W.; Fang, F.; Sheng, W.-T.; Shu, X.; Li, J. Overexpression of SKP2 Inhibits the Radiation-Induced Bystander Effects of Esophageal Carcinoma. Int. J. Environ. Res. Public Health 2017, 14, 155. https://doi.org/10.3390/ijerph14020155

AMA Style

Wang X-C, Zhang T-J, Guo Z-J, Xiao C-Y, Ding X-W, Fang F, Sheng W-T, Shu X, Li J. Overexpression of SKP2 Inhibits the Radiation-Induced Bystander Effects of Esophageal Carcinoma. International Journal of Environmental Research and Public Health. 2017; 14(2):155. https://doi.org/10.3390/ijerph14020155

Chicago/Turabian Style

Wang, Xiao-Chun, Tie-Jun Zhang, Zi-Jian Guo, Chang-Yan Xiao, Xiao-Wen Ding, Fang Fang, Wen-Tao Sheng, Xu Shu, and Jue Li. 2017. "Overexpression of SKP2 Inhibits the Radiation-Induced Bystander Effects of Esophageal Carcinoma" International Journal of Environmental Research and Public Health 14, no. 2: 155. https://doi.org/10.3390/ijerph14020155

APA Style

Wang, X.-C., Zhang, T.-J., Guo, Z.-J., Xiao, C.-Y., Ding, X.-W., Fang, F., Sheng, W.-T., Shu, X., & Li, J. (2017). Overexpression of SKP2 Inhibits the Radiation-Induced Bystander Effects of Esophageal Carcinoma. International Journal of Environmental Research and Public Health, 14(2), 155. https://doi.org/10.3390/ijerph14020155

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