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Volume 14
Issue 12
10.3390/ijerph14121438
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Article

Optimization of the Use of His6-OPH-Based Enzymatic Biocatalysts for the Destruction of Chlorpyrifos in Soil

by
Olga Senko
,
Olga Maslova
and
Elena Efremenko
*
Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Assaf A. Abdelghani
Int. J. Environ. Res. Public Health 2017, 14(12), 1438; https://doi.org/10.3390/ijerph14121438
Received: 30 October 2017 / Revised: 16 November 2017 / Accepted: 21 November 2017 / Published: 23 November 2017
(This article belongs to the Special Issue The Impact of Agricultural Pesticides on Human Health and the Environment)
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Abstract

Applying enzymatic biocatalysts based on hexahistidine-containing organophosphorus hydrolase (His6-OPH) is suggested for the decomposition of chlorpyrifos, which is actively used in agriculture in many countries. The application conditions were optimized and the following techniques was suggested to ensure the highest efficiency of the enzyme: first, the soil is alkalinized with hydrated calcitic lime Ca(OH)2, then the enzyme is introduced into the soil at a concentration of 1000 U/kg soil. Non-equilibrium low temperature plasma (NELTP)-modified zeolite is used for immobilization of the relatively inexpensive polyelectrolyte complexes containing the enzyme His6-OPH and a polyanionic polymer: poly-l-glutamic acid (PLE50) or poly-l-aspartic acid (PLD50). The soil’s humidity is then increased up to 60–80%, the top layer (10–30 cm) of soil is thoroughly stirred, and then exposed for 48–72 h. The suggested approach ensures 100% destruction of the pesticide within 72 h in soils containing as much as 100 mg/kg of chlorpyrifos. It was concluded that using this type of His6-OPH-based enzyme chemical can be the best approach for soils with relatively low humus concentrations, such as sandy and loam-sandy chestnut soils, as well as types of soil with increased alkalinity (pH 8.0–8.4). Such soils are often encountered in desert, desert-steppe, foothills, and subtropical regions where chlorpyrifos is actively used. View Full-Text
Keywords: hexahistidine-containing organophosphorus hydrolase; soil; chlorpyrifos biodegradation; Dursban; agricultural pesticides; organophosphorus and organochlorine compounds hexahistidine-containing organophosphorus hydrolase; soil; chlorpyrifos biodegradation; Dursban; agricultural pesticides; organophosphorus and organochlorine compounds
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MDPI and ACS Style

Senko, O.; Maslova, O.; Efremenko, E. Optimization of the Use of His6-OPH-Based Enzymatic Biocatalysts for the Destruction of Chlorpyrifos in Soil. Int. J. Environ. Res. Public Health 2017, 14, 1438. https://doi.org/10.3390/ijerph14121438

AMA Style

Senko O, Maslova O, Efremenko E. Optimization of the Use of His6-OPH-Based Enzymatic Biocatalysts for the Destruction of Chlorpyrifos in Soil. International Journal of Environmental Research and Public Health. 2017; 14(12):1438. https://doi.org/10.3390/ijerph14121438

Chicago/Turabian Style

Senko, Olga, Olga Maslova, and Elena Efremenko. 2017. "Optimization of the Use of His6-OPH-Based Enzymatic Biocatalysts for the Destruction of Chlorpyrifos in Soil" International Journal of Environmental Research and Public Health 14, no. 12: 1438. https://doi.org/10.3390/ijerph14121438

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