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Open AccessArticle

Effects of Ambient Fine Particles PM2.5 on Human HaCaT Cells

by Qiao Li 1,†, Zhihua Kang 2,†, Shuo Jiang 3,†, Jinzhuo Zhao 3, Shuxian Yan 1, Feng Xu 1,* and Jinhua Xu 1,*
Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China
Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this study.
Academic Editor: William Toscano
Int. J. Environ. Res. Public Health 2017, 14(1), 72;
Received: 3 October 2016 / Revised: 23 December 2016 / Accepted: 2 January 2017 / Published: 12 January 2017
(This article belongs to the Section Global Health)
The current study was conducted to observe the effects of fine particulate matter (PM2.5) on human keratinocyte cell line (HaCaT) cells. The potential mechanism linking PM2.5 and skin was explored. HaCaT cells were cultured and then accessed in plate with PM2.5. Cell viability was tested by Cell Counting Kit-8. The mRNA and protein expression of Filaggrin, Loricrin, Involucrin, and Repetin were analyzed. The levels of Granulocyte-macrophage Colony Stimulating Factor, Thymic Stromal Lymphopoietin, Tumor Necrosis Factor-α, Interleukin-1α, and Interleukin-8 were detected in the supernatant of the HaCaT cell with enzyme-linked immunosorbent assay kits. Cell viability decreased with the increase in PM2.5. Compared with the control group, the protein expression of Filaggrin, Repetin, Involucrin, and Loricrin showed different expression patterns in PM2.5 treatment groups. The level of Tumor Necrosis Factor-α, Thymic Stromal Lymphopoietin, Interleukin-1α, and Interleukin-8 significantly increased in the cells treated with PM2.5. Ambient PM2.5 may increase the risk of eczema and other skin diseases. The relative mechanism may be associated with the impairment of the skin barrier and the elevation of inflammatory responses. View Full-Text
Keywords: PM2.5; HaCaT cell; skin damage PM2.5; HaCaT cell; skin damage
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MDPI and ACS Style

Li, Q.; Kang, Z.; Jiang, S.; Zhao, J.; Yan, S.; Xu, F.; Xu, J. Effects of Ambient Fine Particles PM2.5 on Human HaCaT Cells. Int. J. Environ. Res. Public Health 2017, 14, 72.

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