Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy
AbstractFree-living amoebae (FLA) are protozoa ubiquitous in Nature, isolated from a variety of environments worldwide. In addition to their natural distribution, some species have been found to be pathogenic to humans. In the present study a survey was conducted in order to evaluate the presence and to characterize at molecular level the isolates of amoebic organisms collected from different water sources in Italy. A total of 160 water samples were analyzed by culture and microscopic examination. FLA were found in 46 (28.7%) of the investigated water samples. Groundwater, well waters, and ornamental fountain waters were the sources with higher prevalence rates (85.7%, 50.0%, and 45.9%, respectively). Identification of FLA species/genotypes, based on the 18S rDNA regions, allowed to identify 18 (39.1%) Acanthamoeba isolates (genotypes T4 and T15) and 21 (45.6%) Vermamoeba vermiformis isolates. Other FLA species, including Vahlkampfia sp. and Naegleria spp., previously reported in Italy, were not recovered. The occurrence of potentially pathogenic free-living amoebae in habitats related to human population, as reported in the present study, supports the relevance of FLA as a potential health threat to humans. View Full-Text
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Montalbano Di Filippo, M.; Santoro, M.; Lovreglio, P.; Monno, R.; Capolongo, C.; Calia, C.; Fumarola, L.; D'Alfonso, R.; Berrilli, F.; Di Cave, D. Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy. Int. J. Environ. Res. Public Health 2015, 12, 3417-3427.
Montalbano Di Filippo M, Santoro M, Lovreglio P, Monno R, Capolongo C, Calia C, Fumarola L, D'Alfonso R, Berrilli F, Di Cave D. Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy. International Journal of Environmental Research and Public Health. 2015; 12(4):3417-3427.Chicago/Turabian Style
Montalbano Di Filippo, Margherita; Santoro, Maristella; Lovreglio, Piero; Monno, Rosa; Capolongo, Carmen; Calia, Carla; Fumarola, Luciana; D'Alfonso, Rossella; Berrilli, Federica; Di Cave, David. 2015. "Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy." Int. J. Environ. Res. Public Health 12, no. 4: 3417-3427.