Previous Article in Journal
Biodiversity and Biological Interactions of Actinobacteria Associated with Deep Sea and Intertidal Marine Invertebrates
Previous Article in Special Issue
Anthoteibinenes F–Q: New Sesquiterpenes from the Irish Deep-Sea Coral Anthothela grandiflora
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Marine Drugs
Volume 23
Issue 10
10.3390/md23100409
marinedrugs-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

High-Seas Marine Microorganism Delivers an Extract That Dampens LPS-Driven Pro-Inflammatory Signaling: Galbibacter orientalis Strain ROD011

by
Minji Kim
1,
You-Jin Jeon
2,
Bomi Ryu
3,
Young-Mog Kim
1,4,
Jae-Il Kim
3,
Minkyeong Choi
5,
Sohee Kim
5,
Jihye Lee
5,* and
Jimin Hyun
3,*
1
Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea
2
Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea
3
Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea
4
Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea
5
Laboratories of Marine New Drugs, Redone Technologies Co., Ltd., Jangseong-gun 57247, Republic of Korea
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2025, 23(10), 409; https://doi.org/10.3390/md23100409
Submission received: 15 September 2025 / Revised: 15 October 2025 / Accepted: 16 October 2025 / Published: 18 October 2025
(This article belongs to the Special Issue Bioactive Molecules from Extreme Environments III)
Versions Notes

Abstract

An ethyl acetate extract from the deep-sea bacterium Galbibacter orientalis strain ROD011 (GOEE), collected from international waters, was investigated as a potential anti-inflammatory agent. In lipopolysaccharide (LPS)-stimulated murine macrophages, nitric oxide (NO) production fell by 72–87% at 5–20 µg/mL GOEE without detectable cytotoxicity. Cyclooxygenase-2 (COX-2 protein abundance decreased in a dose-dependent manner and was nearly absent at 20 µg/mL. In zebrafish embryos, survival was maintained up to 40 µg/mL, and LPS-induced signals were attenuated; the cell-death rate declined from 10 µg/mL onward, and at 20 µg/mL GOEE, reactive oxygen species (ROS) and NO decreased by 85% and 27%, respectively. To explain these effects, untargeted metabolomics with pathway enrichment and network mapping were performed in LPS-driven macrophages. Of the 58 KEGG pathways evaluated, 18 reached significance, notably purine and pyrimidine metabolism, vitamin B6 metabolism, and the one-carbon pool via folate. Coordinated shifts also involved amino-acid/tricarboxylic acid (TCA)-cycle linkages, glutathione and glyoxylate/dicarboxylate, and sphingolipid pathways. Network analysis identified hubs that were concomitantly reprogrammed. Collectively, GOEE achieved multi-level suppression of inflammatory outputs while preserving viability, and the metabolomic signature provides a mechanistic scaffold for its action. These findings nominate a deep-sea microbial extract as a promising anti-inflammatory lead and motivate fractionation and targeted validation of the highlighted metabolic nodes.
Keywords: high-seas resources; Galbibacter orientalis strain ROD011; deep-sea microbiome; anti-inflammatory activity; metabolomics; RAW 264.7 macrophages; zebrafish high-seas resources; Galbibacter orientalis strain ROD011; deep-sea microbiome; anti-inflammatory activity; metabolomics; RAW 264.7 macrophages; zebrafish

Share and Cite

MDPI and ACS Style

Kim, M.; Jeon, Y.-J.; Ryu, B.; Kim, Y.-M.; Kim, J.-I.; Choi, M.; Kim, S.; Lee, J.; Hyun, J. High-Seas Marine Microorganism Delivers an Extract That Dampens LPS-Driven Pro-Inflammatory Signaling: Galbibacter orientalis Strain ROD011. Mar. Drugs 2025, 23, 409. https://doi.org/10.3390/md23100409

AMA Style

Kim M, Jeon Y-J, Ryu B, Kim Y-M, Kim J-I, Choi M, Kim S, Lee J, Hyun J. High-Seas Marine Microorganism Delivers an Extract That Dampens LPS-Driven Pro-Inflammatory Signaling: Galbibacter orientalis Strain ROD011. Marine Drugs. 2025; 23(10):409. https://doi.org/10.3390/md23100409

Chicago/Turabian Style

Kim, Minji, You-Jin Jeon, Bomi Ryu, Young-Mog Kim, Jae-Il Kim, Minkyeong Choi, Sohee Kim, Jihye Lee, and Jimin Hyun. 2025. "High-Seas Marine Microorganism Delivers an Extract That Dampens LPS-Driven Pro-Inflammatory Signaling: Galbibacter orientalis Strain ROD011" Marine Drugs 23, no. 10: 409. https://doi.org/10.3390/md23100409

APA Style

Kim, M., Jeon, Y.-J., Ryu, B., Kim, Y.-M., Kim, J.-I., Choi, M., Kim, S., Lee, J., & Hyun, J. (2025). High-Seas Marine Microorganism Delivers an Extract That Dampens LPS-Driven Pro-Inflammatory Signaling: Galbibacter orientalis Strain ROD011. Marine Drugs, 23(10), 409. https://doi.org/10.3390/md23100409

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop