Effects of Epigenetic Modification and High Hydrostatic Pressure on Polyketide Synthase Genes and Secondary Metabolites of Alternaria alternata Derived from the Mariana Trench Sediments
Abstract
:1. Introduction
2. Results
2.1. Isolation and Identification of Hadal Sediment-Derived Fungi
2.2. Screening of the PKS Genes in Hadal-Derived Fungi
2.3. Chemical Epigenetic Modifier Affects Fungal Phenotype
2.4. Chemical Epigenetic Modifier Affects PKS Gene Expression in Fungi
2.5. Chemical Epigenetic Modifier Affects the Components of Fungal Secondary Metabolites
2.6. Chemical Epigenetic Modifier Affects Antimicrobial Activity
2.7. Chemical Epigenetic Modifier Affects PKS Gene Expression under HHP Conditions
2.8. Chemical Epigenetic Modifier Affects the Components of Secondary Metabolites under HHP Conditions
2.9. Chemical Epigenetic Modifier Affects Antimicrobial Activity under HHP Conditions
3. Discussion
4. Materials and Methods
4.1. Fungal Isolation and Purification
4.2. DNA Extraction, rDNA-ITS Gene Screening and Phylogenetic Analysis
4.3. PKS Gene Screening and Phylogenetic Analysis
4.4. Fermentation Culture of Chemical Epigenetic Modifiers of Hadal-Derived Fungi
4.5. High Hydrostatic Pressures Culture with Chemical Epigenetic Modifiers of Hadal-Derived Fungi
4.6. RNA Extraction and Reverse Transcription
4.7. Primer Design and Quantitative Reverse Transcriptase PCR (qRT-PCR)
4.8. Extraction of Secondary Metabolites of Hadal-Derived Fungi
4.9. UPLC-MS/MS Analysis of Secondary Metabolites from Hadal-Derived Fungi
4.10. Antimicrobial Activity Assay
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Peng, Q.; Li, Y.; Fang, J.; Yu, X. Effects of Epigenetic Modification and High Hydrostatic Pressure on Polyketide Synthase Genes and Secondary Metabolites of Alternaria alternata Derived from the Mariana Trench Sediments. Mar. Drugs 2023, 21, 585. https://doi.org/10.3390/md21110585
Peng Q, Li Y, Fang J, Yu X. Effects of Epigenetic Modification and High Hydrostatic Pressure on Polyketide Synthase Genes and Secondary Metabolites of Alternaria alternata Derived from the Mariana Trench Sediments. Marine Drugs. 2023; 21(11):585. https://doi.org/10.3390/md21110585
Chicago/Turabian StylePeng, Qingqing, Yongqi Li, Jiasong Fang, and Xi Yu. 2023. "Effects of Epigenetic Modification and High Hydrostatic Pressure on Polyketide Synthase Genes and Secondary Metabolites of Alternaria alternata Derived from the Mariana Trench Sediments" Marine Drugs 21, no. 11: 585. https://doi.org/10.3390/md21110585