Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. General Methods
3.2. Isolation of Polysaccharides
3.3. Dilute Acid Treatment of Samples
3.4. Agarose Gel Electrophoresis (PAGE)
3.5. NMR Spectroscopy
3.6. Anticoagulant Activity Measured in Clotting Time Test
3.7. Effect of Polysaccharides on Thrombin or Factor Xa Inactivation by Antithrombin III
3.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample | Fuc | Gal | GlcNAc | GalNAc | UA, Na-salt | SO3Na | Molecular Weight, kDa (Dispercity) |
---|---|---|---|---|---|---|---|
SP-BA | 1.00 | 0.14 | 0.07 | 0.30 | 0.30 | 2.05 | |
FCS-BA | 1.00 | 0.09 | n.d. | 0.78 | 0.83 | 4.04 | 32 (1.55) |
FS-BA-AT | 1.00 | 0.09 | 0.04 | 0.06 | 0.04 | 1.28 | 55 (1.35) |
SP-HS | 1.00 | 0.12 | 0.08 | 0.3 | 0.21 | 2.44 | |
FCS-HS | 1.00 | 0.12 | 0.04 | 0.74 | 1.10 | 3.44 | 30 (1.62) |
FS-HS-AT | 1.00 | 0.09 | 0.04 | 0.18 | 0.18 | 1.69 |
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Ustyuzhanina, N.E.; Bilan, M.I.; Dmitrenok, A.S.; Tsvetkova, E.A.; Nikogosova, S.P.; Hang, C.T.T.; Thinh, P.D.; Trung, D.T.; Van, T.T.T.; Shashkov, A.S.; et al. Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity. Mar. Drugs 2022, 20, 380. https://doi.org/10.3390/md20060380
Ustyuzhanina NE, Bilan MI, Dmitrenok AS, Tsvetkova EA, Nikogosova SP, Hang CTT, Thinh PD, Trung DT, Van TTT, Shashkov AS, et al. Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity. Marine Drugs. 2022; 20(6):380. https://doi.org/10.3390/md20060380
Chicago/Turabian StyleUstyuzhanina, Nadezhda E., Maria I. Bilan, Andrey S. Dmitrenok, Eugenia A. Tsvetkova, Sofya P. Nikogosova, Cao Thi Thuy Hang, Pham Duc Thinh, Dinh Thanh Trung, Tran Thi Thanh Van, Alexander S. Shashkov, and et al. 2022. "Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity" Marine Drugs 20, no. 6: 380. https://doi.org/10.3390/md20060380
APA StyleUstyuzhanina, N. E., Bilan, M. I., Dmitrenok, A. S., Tsvetkova, E. A., Nikogosova, S. P., Hang, C. T. T., Thinh, P. D., Trung, D. T., Van, T. T. T., Shashkov, A. S., Usov, A. I., & Nifantiev, N. E. (2022). Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity. Marine Drugs, 20(6), 380. https://doi.org/10.3390/md20060380