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Article

Extraction and Identification of Three New Urechis unicinctus Visceral Peptides and Their Antioxidant Activity

1
Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2
Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), The Ministry of Agriculture and Rural Affairs of the P.R. China, Beijing 100083, China
3
Shandong Baier Testing Corp., Ltd., Weifang 261061, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Bin Wang and Chang-Feng Chi
Mar. Drugs 2022, 20(5), 293; https://doi.org/10.3390/md20050293
Received: 22 March 2022 / Revised: 13 April 2022 / Accepted: 13 April 2022 / Published: 27 April 2022
(This article belongs to the Special Issue Marine Bioactive Peptides—Structure, Function, and Application)
The viscera of Urechis unicinctus with polypeptides, fatty acids, and amino acids are usually discarded during processing to food. In order to improve the utilization value of the viscera of Urechis unicinctus and avoid resource waste, antioxidant polypeptides were isolated from the viscera of Urechis unicinctus. First, a protein hydrolysate of Urechis unicinctus (UUPH) was prepared by ultrasonic-assisted enzymatic hydrolysis, and the degree of hydrolysis was as high as 79.32%. Subsequently, three new antioxidant peptides (P1, P2, and P3) were purified from UUPH using ultrafiltration and chromatography, and their amino acid sequences were identified as VTSALVGPR, IGLGDEGLRR, TKIRNEISDLNER, respectively. Then, the antioxidant activity of the polypeptide was predicted by the structure–activity relationship and finally verified by experiments on eukaryotic cells. The P1 peptide exhibited the strongest antioxidant activity among these three antioxidant peptides. Furthermore, P1, P2, and P3 have no toxic effect on RAW264.7 cells at the concentration of 0.01~2 mg/mL and can protect RAW264.7 cells from H2O2-induced oxidative damage in a concentration-dependent manner. These results suggested that these three new antioxidant peptides were isolated from the viscera of Urechis unicinctus, especially the P1 peptide, which might serve as potential antioxidants applied in health-derived food or beverages. This study further developed a new use of the by-product of Urechis unicinctus, which improved the comprehensive utilization of marine biological resources. View Full-Text
Keywords: internal organs of Urechis unicinctus; ultrasound-assisted enzymatic hydrolysis; polypeptide; antioxidant activity internal organs of Urechis unicinctus; ultrasound-assisted enzymatic hydrolysis; polypeptide; antioxidant activity
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MDPI and ACS Style

Li, J.; Lu, J.; Asakiya, C.; Huang, K.; Zhou, X.; Liu, Q.; He, X. Extraction and Identification of Three New Urechis unicinctus Visceral Peptides and Their Antioxidant Activity. Mar. Drugs 2022, 20, 293. https://doi.org/10.3390/md20050293

AMA Style

Li J, Lu J, Asakiya C, Huang K, Zhou X, Liu Q, He X. Extraction and Identification of Three New Urechis unicinctus Visceral Peptides and Their Antioxidant Activity. Marine Drugs. 2022; 20(5):293. https://doi.org/10.3390/md20050293

Chicago/Turabian Style

Li, Jingjing, Jiajun Lu, Charles Asakiya, Kunlun Huang, Xiuzhi Zhou, Qingliang Liu, and Xiaoyun He. 2022. "Extraction and Identification of Three New Urechis unicinctus Visceral Peptides and Their Antioxidant Activity" Marine Drugs 20, no. 5: 293. https://doi.org/10.3390/md20050293

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