One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography
Abstract
:1. Introduction
2. Results and Discussions
2.1. Selection of the Two-Phase Solvent System
2.2. Optimization of Elution-Extrusion Countercurrent Chromatography
2.2.1. Effect of the Flow Rate of the Mobile Phase on the Retention of the Stationary Phase
2.2.2. Effect of Revolution Speed on the Retention of the Stationary Phase
2.3. Elution-Extrusion Countercurrent Chromatography Separation of Fucoxanthin
2.4. Analysis of HPLC and UV-Vis Full Scan
3. Materials and Methods
3.1. Materials and Reagents
3.2. Preparation of Crude Extract
3.3. Quantitation of Fucoxanthin by High-Performance Liquid Chromatography
3.4. Selection of the Two-Phase Solvent System
3.5. Preparation of Elution Solvent and Sample Solution
3.6. Optimization of Elution-Extrusion Countercurrent Chromatography
3.7. Verification of the Optimal EECCC Method
3.8. Detection of Elution-Extrusion Countercurrent Chromatography Fractions
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Solvent System | Volume Ratios | K |
---|---|---|
n-hexane-ethanol-water | 20:11:9 | 0.23 ± 0.09 |
20:10:10 | 0.65 ± 0.08 | |
20:9:11 | 1.74 ± 0.11 | |
20:8:12 | 2.28 ± 0.12 | |
20:7:13 | 3.61 ± 0.14 |
Samples | Loading Capacity (mg/10 mL) | Yield (mg) | Purity (%) |
---|---|---|---|
S. fusiforme | 50 | 11.3 b,c,* | 96.01 ± 1.80 A ** |
60 | 12.8 b | 94.72 ± 1.47 A | |
80 | 16.7 a | 90.51 ± 1.53 C | |
100 | 18.1 a | 85.37 ± 1.63 D | |
L. japonica | 60 | 6.0 d | 96.24 ± 1.58 A |
U. pinnatifida | 60 | 9.7 c | 92.62 ± 1.77 B |
Repeatability (RSD * %, N = 3) | Precision (RSD%, N = 6) | |
---|---|---|
Yield (mg) | 5.2 | 5.9 |
Purity (%) | 1.6 | 2.3 |
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Chen, D.; Jin, Y.; Hu, D.; Ye, J.; Lu, Y.; Dai, Z. One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography. Mar. Drugs 2022, 20, 257. https://doi.org/10.3390/md20040257
Chen D, Jin Y, Hu D, Ye J, Lu Y, Dai Z. One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography. Marine Drugs. 2022; 20(4):257. https://doi.org/10.3390/md20040257
Chicago/Turabian StyleChen, Danting, Yating Jin, Di Hu, Jing Ye, Yanbin Lu, and Zhiyuan Dai. 2022. "One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography" Marine Drugs 20, no. 4: 257. https://doi.org/10.3390/md20040257