AsKC11, a Kunitz Peptide from Anemonia sulcata, Is a Novel Activator of G Protein-Coupled Inward-Rectifier Potassium Channels
Abstract
:1. Introduction
2. Results
2.1. Enhancement of K+ Currents through GIRK1/2 Channels by the Venom Fraction from Anemonia Sulcata
2.2. Purification and Identification of AsKC11 from the Active Venom Fraction
2.3. Potency of AsKC11 at Enhancing GIRK1/2 Channel-Carried Inward K+ Currents
2.4. AsKC11-Evoked Activation of GIRK1/2 Channels Independent from Gi/o
2.5. Affinity of AsKC11 to GIRK1/2 Channels
2.6. Effect of AsKC11 on Different Potassium Ion Channels
3. Discussion
4. Materials and Methods
4.1. AsKC11 Identification
4.2. Recombinant Synthesis of rAsKC11
4.3. Oocyte Preparation
4.4. Electrophysiological Measurements
4.5. Data Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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An, D.; Pinheiro-Junior, E.L.; Béress, L.; Gladkikh, I.; Leychenko, E.; Undheim, E.A.B.; Peigneur, S.; Tytgat, J. AsKC11, a Kunitz Peptide from Anemonia sulcata, Is a Novel Activator of G Protein-Coupled Inward-Rectifier Potassium Channels. Mar. Drugs 2022, 20, 140. https://doi.org/10.3390/md20020140
An D, Pinheiro-Junior EL, Béress L, Gladkikh I, Leychenko E, Undheim EAB, Peigneur S, Tytgat J. AsKC11, a Kunitz Peptide from Anemonia sulcata, Is a Novel Activator of G Protein-Coupled Inward-Rectifier Potassium Channels. Marine Drugs. 2022; 20(2):140. https://doi.org/10.3390/md20020140
Chicago/Turabian StyleAn, Dongchen, Ernesto Lopes Pinheiro-Junior, László Béress, Irina Gladkikh, Elena Leychenko, Eivind A. B. Undheim, Steve Peigneur, and Jan Tytgat. 2022. "AsKC11, a Kunitz Peptide from Anemonia sulcata, Is a Novel Activator of G Protein-Coupled Inward-Rectifier Potassium Channels" Marine Drugs 20, no. 2: 140. https://doi.org/10.3390/md20020140