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Article

Changes in the Molecular Characteristics of Bovine and Marine Collagen in the Presence of Proteolytic Enzymes as a Stage Used in Scaffold Formation

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Federal State Budgetary Educational Institution of Higher Education, Privolzhsky Research Medical University, the Ministry of Health of the Russian Federation (FSBEI HE PRMU MOH), Minin and Pozharsky Square 10/1, 603950 Nizhny Novgorod, Russia
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Faculty of Chemistry, Lobachevsky State University of Nizhny Novgorod, pr. Gagarina 23, 603950 Nizhny Novgorod, Russia
*
Author to whom correspondence should be addressed.
Academic Editors: Marco Patruno, Michela Sugni, Luca Melotti and Hitoshi Sashiwa
Mar. Drugs 2021, 19(9), 502; https://doi.org/10.3390/md19090502
Received: 19 July 2021 / Revised: 25 August 2021 / Accepted: 31 August 2021 / Published: 2 September 2021
(This article belongs to the Special Issue Regenerative Potential of Marine Natural Compounds)
Biopolymers, in particular collagen and fibrinogen, are the leading materials for use in tissue engineering. When developing technology for scaffold formation, it is important to understand the properties of the source materials as well as the mechanisms that determine the formation of the scaffold structures. Both factors influence the properties of scaffolds to a great extent. Our present work aimed to identify the features of the molecular characteristics of collagens of different species origin and the changes they undergo during the enzymatic hydrolysis used for the process of scaffold formation. For this study, we used the methods of gel-penetrating chromatography, dynamic light scattering, reading IR spectra, and scanning electron microscopy. It was found that cod collagen (CC) and bovine collagen (BC) have different initial molecular weight parameters, and that, during hydrolysis, the majority of either type of protein is hydrolyzed by the proteolytic enzymes within the first minute. The differently sourced collagen samples were also hydrolyzed with the formation of two low molecular fractions: Mw ~ 10 kDa and ~20 kDa. In the case of CC, the microstructure of the final scaffolds contained denser, closely spaced fibrillar areas, while the BC-sourced scaffolds had narrow, short fibrils composed of unbound fibers of hydrolyzed collagen in their structure. View Full-Text
Keywords: scaffold; biopolymers; collagen; fibrinogen; hydrolysis; thrombin; pancreatin scaffold; biopolymers; collagen; fibrinogen; hydrolysis; thrombin; pancreatin
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MDPI and ACS Style

Egorikhina, M.N.; Semenycheva, L.L.; Chasova, V.O.; Bronnikova, I.I.; Rubtsova, Y.P.; Zakharychev, E.A.; Aleynik, D.Y. Changes in the Molecular Characteristics of Bovine and Marine Collagen in the Presence of Proteolytic Enzymes as a Stage Used in Scaffold Formation. Mar. Drugs 2021, 19, 502. https://doi.org/10.3390/md19090502

AMA Style

Egorikhina MN, Semenycheva LL, Chasova VO, Bronnikova II, Rubtsova YP, Zakharychev EA, Aleynik DY. Changes in the Molecular Characteristics of Bovine and Marine Collagen in the Presence of Proteolytic Enzymes as a Stage Used in Scaffold Formation. Marine Drugs. 2021; 19(9):502. https://doi.org/10.3390/md19090502

Chicago/Turabian Style

Egorikhina, Marfa N., Ludmila L. Semenycheva, Victoria O. Chasova, Irina I. Bronnikova, Yulia P. Rubtsova, Evgeniy A. Zakharychev, and Diana Y. Aleynik. 2021. "Changes in the Molecular Characteristics of Bovine and Marine Collagen in the Presence of Proteolytic Enzymes as a Stage Used in Scaffold Formation" Marine Drugs 19, no. 9: 502. https://doi.org/10.3390/md19090502

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