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Article

Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin

1
Service d’Ingénierie Moléculaire Pour la Santé (SIMoS), Département Médicaments et Technologies Pour la Santé (DMTS), Institut des Sciences du Vivant Frédéric Joliot, Université Paris-Saclay, CEA, ERL CNRS/CEA 9004, 91191 Gif-sur-Yvette, France
2
Laboratoire des Sciences de l’Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, 29280 Plouzané, France
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Centre Ifremer Bretagne, Ifremer, UMR 6539 (LEMAR) CNRS/UBO/IRD/Ifremer, ZI de la Pointe du Diable, CS 10070, 29280 Plouzané, France
*
Author to whom correspondence should be addressed.
Academic Editor: Jordi Molgó
Mar. Drugs 2021, 19(7), 380; https://doi.org/10.3390/md19070380
Received: 23 May 2021 / Revised: 23 June 2021 / Accepted: 27 June 2021 / Published: 30 June 2021
(This article belongs to the Special Issue Marine Biotoxins)
Pacific oysters (Crassostrea gigas) may bio-accumulate high levels of paralytic shellfish toxins (PST) during harmful algal blooms of the genus Alexandrium. These blooms regularly occur in coastal waters, affecting oyster health and marketability. The aim of our study was to analyse the PST-sensitivity of nerves of Pacific oysters in relation with toxin bio-accumulation. The results show that C. gigas nerves have micromolar range of saxitoxin (STX) sensitivity, thus providing intermediate STX sensitivity compared to other bivalve species. However, theses nerves were much less sensitive to tetrodotoxin. The STX-sensitivity of compound nerve action potential (CNAP) recorded from oysters experimentally fed with Alexandrium minutum (toxic-alga-exposed oysters), or Tisochrysis lutea, a non-toxic microalga (control oysters), revealed that oysters could be separated into STX-resistant and STX-sensitive categories, regardless of the diet. Moreover, the percentage of toxin-sensitive nerves was lower, and the STX concentration necessary to inhibit 50% of CNAP higher, in recently toxic-alga-exposed oysters than in control bivalves. However, no obvious correlation was observed between nerve sensitivity to STX and the STX content in oyster digestive glands. None of the nerves isolated from wild and farmed oysters was detected to be sensitive to tetrodotoxin. In conclusion, this study highlights the good potential of cerebrovisceral nerves of Pacific oysters for electrophysiological and pharmacological studies. In addition, this study shows, for the first time, that C. gigas nerves have micromolar range of STX sensitivity. The STX sensitivity decreases, at least temporary, upon recent oyster exposure to dinoflagellates producing PST under natural, but not experimental environment. View Full-Text
Keywords: Crassostrea gigas; compound nerve action potential; Alexandrium minutum; paralytic shellfish toxins Crassostrea gigas; compound nerve action potential; Alexandrium minutum; paralytic shellfish toxins
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MDPI and ACS Style

Boullot, F.; Fabioux, C.; Hégaret, H.; Boudry, P.; Soudant, P.; Benoit, E. Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin. Mar. Drugs 2021, 19, 380. https://doi.org/10.3390/md19070380

AMA Style

Boullot F, Fabioux C, Hégaret H, Boudry P, Soudant P, Benoit E. Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin. Marine Drugs. 2021; 19(7):380. https://doi.org/10.3390/md19070380

Chicago/Turabian Style

Boullot, Floriane, Caroline Fabioux, Hélène Hégaret, Pierre Boudry, Philippe Soudant, and Evelyne Benoit. 2021. "Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin" Marine Drugs 19, no. 7: 380. https://doi.org/10.3390/md19070380

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