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Article

A Comparative In Vitro Evaluation of the Anti-Inflammatory Effects of a Tisochrysis lutea Extract and Fucoxanthin

1
Department of NEUROFARBA, Section of Pharmacology and Toxicology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
2
Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Piazzale delle Cascine 18, 50144 Florence, Italy
3
Fotosintetica Microbiologica S.r.l., Via di Santo Spirito, 14, 50125 Florence, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Hitoshi Sashiwa
Mar. Drugs 2021, 19(6), 334; https://doi.org/10.3390/md19060334
Received: 24 May 2021 / Revised: 7 June 2021 / Accepted: 8 June 2021 / Published: 11 June 2021
(This article belongs to the Special Issue Marine Anti-inflammatory and Antioxidant Agents 2021)
In this study, we compared the effects of a Tisochrysis lutea (T. lutea) F&M-M36 methanolic extract with those of fucoxanthin (FX) at equivalent concentration, on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The T. lutea F&M-M36 methanolic extract contained 4.7 mg of FX and 6.22 mg of gallic acid equivalents of phenols per gram. HPLC analysis revealed the presence of simple phenolic acid derivatives. The T. lutea F&M-M36 extract exhibited a potent and concentration-dependent inhibitory activity against COX-2 dependent PGE2 production compared to FX alone. Compared to LPS, T. lutea F&M-M36 extract and FX reduced the expression of IL-6 and of Arg1 and enhanced that of IL-10 and of HO-1; T. lutea F&M-M36 extract also significantly abated the expression of NLRP3, enhanced mir-223 expression and reduced that of mir-146b, compared to LPS (p < 0.05). These findings indicate that T. lutea F&M-M36 methanolic extract has a peculiar anti-inflammatory activity against COX-2/PGE2 and NLRP3/mir-223 that might be attributable to the known anti-inflammatory effects of simple phenolic compounds found in the extract that may synergize with FX. Our data suggest that T. lutea F&M-M36 may serve as a source of anti-inflammatory compounds to be further evaluated in in vivo models of inflammation. View Full-Text
Keywords: microalgae; Tisochrysis lutea; fucoxanthin; inflammation; RAW 264.7; microRNA microalgae; Tisochrysis lutea; fucoxanthin; inflammation; RAW 264.7; microRNA
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MDPI and ACS Style

Bigagli, E.; D’Ambrosio, M.; Cinci, L.; Niccolai, A.; Biondi, N.; Rodolfi, L.; Dos Santos Nascimiento, L.B.; Tredici, M.R.; Luceri, C. A Comparative In Vitro Evaluation of the Anti-Inflammatory Effects of a Tisochrysis lutea Extract and Fucoxanthin. Mar. Drugs 2021, 19, 334. https://doi.org/10.3390/md19060334

AMA Style

Bigagli E, D’Ambrosio M, Cinci L, Niccolai A, Biondi N, Rodolfi L, Dos Santos Nascimiento LB, Tredici MR, Luceri C. A Comparative In Vitro Evaluation of the Anti-Inflammatory Effects of a Tisochrysis lutea Extract and Fucoxanthin. Marine Drugs. 2021; 19(6):334. https://doi.org/10.3390/md19060334

Chicago/Turabian Style

Bigagli, Elisabetta; D’Ambrosio, Mario; Cinci, Lorenzo; Niccolai, Alberto; Biondi, Natascia; Rodolfi, Liliana; Dos Santos Nascimiento, Luana B.; Tredici, Mario R.; Luceri, Cristina. 2021. "A Comparative In Vitro Evaluation of the Anti-Inflammatory Effects of a Tisochrysis lutea Extract and Fucoxanthin" Mar. Drugs 19, no. 6: 334. https://doi.org/10.3390/md19060334

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