Chitosan Coated Microparticles Enhance Simvastatin Colon Targeting and Pro-Apoptotic Activity
Abstract
:1. Introduction
2. Results
2.1. Preparation and Characterization of the SMV-CHIT-ES100 MPs
2.2. In Vitro Antiproliferative Activity
2.3. Cell Cycle Progression Analysis
2.4. Annexin V–FITC Apoptosis Assay and Caspase 3 Cellular Content
2.5. Realtime X-ray Radiography of Iohexol Formulated in CHIT-ES100 MPs in Rabbits
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. SMV-CHIT-ES100 MPs Preparation
4.3. Characterization of SMV-CHIT-ES100 MPs
4.3.1. Scanning Electron Microscopy
4.3.2. Encapsulation Efficiency
4.3.3. SMV In Vitro Release from SMV-CHIT-ES100 MPs
4.3.4. Cell Culture
4.3.5. Anti-Proliferative Activity
4.3.6. Analysis of Cell Cycle Progression
4.3.7. Annexin V Assay
4.3.8. Caspase-3 Enzyme Assay
4.3.9. Realtime X-ray Radiography of the Contrast Medium Iohexol Formulated in Chitosan-Coated Eudrgit in Rabbits
4.3.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Alhakamy, N.A.; Fahmy, U.A.; Ahmed, O.A.A.; Caruso, G.; Caraci, F.; Asfour, H.Z.; Bakhrebah, M.A.; N. Alomary, M.; Abdulaal, W.H.; Okbazghi, S.Z.; et al. Chitosan Coated Microparticles Enhance Simvastatin Colon Targeting and Pro-Apoptotic Activity. Mar. Drugs 2020, 18, 226. https://doi.org/10.3390/md18040226
Alhakamy NA, Fahmy UA, Ahmed OAA, Caruso G, Caraci F, Asfour HZ, Bakhrebah MA, N. Alomary M, Abdulaal WH, Okbazghi SZ, et al. Chitosan Coated Microparticles Enhance Simvastatin Colon Targeting and Pro-Apoptotic Activity. Marine Drugs. 2020; 18(4):226. https://doi.org/10.3390/md18040226
Chicago/Turabian StyleAlhakamy, Nabil A., Usama A. Fahmy, Osama A. A. Ahmed, Giuseppe Caruso, Filippo Caraci, Hani Z. Asfour, Muhammed A. Bakhrebah, Mohammad N. Alomary, Wesam H. Abdulaal, Solomon Z. Okbazghi, and et al. 2020. "Chitosan Coated Microparticles Enhance Simvastatin Colon Targeting and Pro-Apoptotic Activity" Marine Drugs 18, no. 4: 226. https://doi.org/10.3390/md18040226